Single Copper Atom Photocatalyst Powers an Integrated Catalytic Cascade for Drug-Resistant Bacteria Elimination

Abstract

To address the issue posed by drug-resistant bacteria and inspired by natural antimicrobial enzymes, we report the atomically doped copper on guanine-derived nanosheets (G–Cu) that possess the integrated catalytic cascade property of glucose oxidase and peroxidase, yielding free radicals to eliminate drug-resistant bacteria upon light irradiation. Density functional theory calculations demonstrate that copper could notably promote oxygen activation and H2O2 splitting on the G–Cu complexes. Further all-atom simulation and experimental data indicate that the lysis of bacteria is mainly induced by cell membrane damage and the elevation of intracellular reactive oxygen species. Lastly, the G–Cu complexes efficiently eliminate the staphylococci in the infected wounds and accelerate their closure in a murine model, with negligible side effects on the normal tissues. Therefore, our G–Cu complexes may provide an efficient nonantibiotic alternative to the current treatments for bacterial infections.

Lipid Prodrug Nanoassemblies via Dynamic Covalent Boronates

“…Subsequently, we studied the bactericidal efficacy of Lipid-CIP nanoassemblies against a representative drug-resistant bacterial stain, Staphylococcus aureus ( S. aureus ) Xen36. − Pristine Lipid nanoassemblies (without the drug loading) showed negligible bacteriostatic and bactericidal efficacy, with minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) higher than 250 μg/mL (Figure S29, Table S3, Supporting Information). The MIC and MBC values of CIP in Lipid-CIP nanoassemblies were 4-fold lower than free CIP and 2-fold lower than CIP-PBA.…”

Dynamic covalent nano-networks comprising antibiotics and polyphenols orchestrate bacterial drug resistance reversal and inflammation alleviation

“…With all the aDCNs in hand, we then studied the bactericidal efficacy of the aDCNs, as summarized in Table S3 . Undoubtedly, PVP, B and C domains demonstrated negligible antimicrobial efficacy with MIC and MBC values higher than 500 μg/mL against the clinically isolated Escherichia coli ( E. coli ) and multi-drug resistant Staphylococcus aureus ( S. aureus Xen36) [ 43 , 49 ]. The clinically isolated E. coli is a polymyxin-resistant strain with a MIC value of 32 μg/mL.…”

Supramolecular Photothermal Cascade Nano‐Reactor Enables Photothermal Effect, Cascade Reaction, and In Situ Hydrogelation for Biofilm‐Associated Tooth‐Extraction Wound Healing

“…The GOx-assisted cascade reaction process of the CPNC, CPNC@GOx, and CPNC@GOx-Fe 2+ in the presence of glucose was verified by the oxidation of 3,3″,5,5″-tetramethylbenzidine (TMB) that reacts with •OH to yield blue oxTMB with a characteristic UV absorption peak at 652 nm (Figure 2g; Figures S13b and S14c, Supporting Information). [49,50] CPNC@GOx-Fe 2+ alone exhibited rapid catalytic activity toward the oxidation of TMB, resulting in a visible blue coloration (Figure S13b, Supporting Information). However, due to the peroxidase-like catalytic activity of CPNC (Figure S14a, Supporting Information), CPNC@GOx can also undergo a GOx-assisted cascade reaction process.…”