Single‐Domain Antibodies: An Overview

Single-Domain Antibodies as Crystallization Chaperones to Enable Structure-Based Inhibitor Development for RBR E3 Ubiquitin Ligases

“…Cameloid-derived nanobodies have proved very useful tools to assist crystallizing complex protein architectures; for example, a ubiquitin-bound kinase PINK (Schubert et al, 2017) and active G protein-coupled receptor conformations (Steyaert and Kobilka, 2011). To avoid the need for immunization, we decided to test if synthetic domain antibody (dAb) libraries based on human scaffolds (Enever et al, 2014;Ignatovich et al, 2012) may also serve as crystallization chaperones. Synthetic libraries have the advantage of overcoming poorly immunogenic targets and allow selection in the presence of ligands; however, use of human dAbs as crystallization chaperones is as yet largely unexplored.…”