Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet

Lestari, Sri Rahayu; Atho'illah, Mochammad Fitri; Christina, Yuyun Ika; Rifa’i, Muhaimin

doi:10.1016/j.jaim.2020.06.009

Cited by 24 publications

(9 citation statements)

References 27 publications

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“…DCs commonly expressed CD11c and MHC class II, and the expression was increased once DCs is activated to activate naïve T cells [ 9 ]. Interestingly, memory CD4 + T cells mainly migrated to inflammatory sites, driving obesity−associated insulin resistance together with CD8 + in adipose tissue [ 53 , 54 ]. In line with our result, HFD resulting in the lower expression of CD4 + CD62L + , thus interrupt the lymphocyte and erythrocyte homeostasis [ 53 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Atho'illah

Safitri²,

Nur’aini

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Background The high−fat, high−fructose diet (HFFD) provokes overnutrition and inflammation directly, mainly through Toll−like receptors (TLRs). Soybean ( Glycine max L.) contains isoflavone that can be transformed into glyceollin by microbial and physical stimuli. Glyceollin possesses many beneficial effects on health. Objective This study evaluates the beneficial effect of soybean extract elicited by Saccharomyces cerevisiae and light (ESE) on dendritic cells (DCs) profile and naïve T cells in HFFD mice. Materials and methods Female Balb/C mice were fed with HFFD for 24 weeks then orally administered with simvastatin 2.8 mg/kg BW or ESE 78, 104, and 130 mg/kg BW at the last four weeks. The expression of splenic CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + , CD4 + CD62L + , and CD8 + CD62L + subsets was measured by flow cytometry. The molecular docking has been measured using Pyrx 0.8, displayed in PyMol and Biovia Discovery Studio. Result HFFD significantly increased CD11c + TLR3 + , CD11c + TLR4 + , NFκB + , CD11c + IL-17 + , CD11c + TNF−α + expression and decreased CD4 + CD62L + and CD8 + CD62L + ( p < 0.05) compared to normal diet (ND) groups. ESE reduced CD11c + TLR3 + , CD11c + TLR4 + , thereby decreasing NFκB + , as well as decreased the CD11c + IL−17 + , CD11c + TNF−α + , and restores CD4 + CD62L + and CD8 + CD62L + subsets in HFFD mice. Glyceollin II exhibited the best binding affinity with an average energy of −7.3 kcal/mol to TLR3 and −7.9 kcal/mol to TLR4. Conclusion The bioactive compound in ESE act synergistically to modulate TLR3/TLR4 activation, reduced NFκB, IL−17, and TNF−α, and restores naïve T cells expression in HFFD mice. ESE was a favorable candidate to mitigate chronic inflammation.

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Section: Discussionmentioning

confidence: 99%

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Atho'illah

Safitri²,

Nur’aini

et al. 2021

Journal of Ayurveda and Integrative Medicine

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“…A study confirmed that Single garlic oil could suppress CD4 t-cells activation and NF-κB Frontiers in Pharmacology frontiersin.org expression in high-fat diet mice. Furthermore, Single garlic oil plays a role as an athero-protective agent in the High-fat diet condition through the decrease in proinflammatory cytokines such as TNF-a and IFN-g (Lestari et al, 2020). SGO could act as a promising prospect for therapy to improve chronic inflammation in AS.…”

Section: Anti-inflammatorymentioning

confidence: 99%

Roles and mechanisms of garlic and its extracts on atherosclerosis: A review

Yun

Wang

et al. 2022

Front. Pharmacol.

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The prevention and treatment of cardiovascular diseases (CVDs) have achieved initial results, but the number of CVDs patients will increase rapidly in the next 10 years. Atherosclerosis (AS) is a significant risk factor for CVDs. The impact of lifestyle and daily diet varies considerably between different countries and continents and has been shown to affect the development of various diseases such as diabetes and CVDs. Primary and secondary prevention using alternative supplements and methods to avoid or reduce the use of traditional pharmacological drugs have also become popular. One of the reasons for this is that pharmacological drugs with lipid-lowering, and blood pressure-lowering effects cause many side effects that may negatively impact the quality of life. Patients are now emphasizing reliance on lifestyle changes to reduce cardiovascular risks. Garlic is a medicinal and edible plant that has been used for a long time. In order to reveal garlic application in the prevention and treatment of AS, reviewing the latest domestic and international studies through searching databases. The result shows that the antiatherogenic role of garlic is eximious. And the mechanisms are mainly related to hypolipidemic, antioxidant, antithrombotic, inhibiting angiogenesis, protecting endothelial cells, anti-inflammatory, anti-apoptotic, inhibiting vascular smooth muscle proliferation, and regulating gut microbiota. The main signaling pathways involve AMPK/TLRs, Keap1/Nrf2, PI3K/AKT, PPARγ/LXRα, GEF-H1/RhoA/Rac, etc. The antiatherogenic actions and molecular mechanism of garlic were reviewed in this study to obtain a robust evidence basis for the clinical application and mechanistic study and provide a theoretical basis for further utilization of garlic.

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“…During bacterial infection, macrophages expressed Toll-like receptors 4 (TLR4), which primarily recognizes the lipopolysaccharide (LPS) as the major component of the cell wall of S. typhi . This recognition subsequently stimulates TLR4 signaling pathways, such as NFκB, to produce proinflammatory cytokines, resulting in increased systemic cytokine production and septic shock in the later stages [ [8] , [9] , [10] ].…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus plantarum FNCC 0137 fermented red Moringa oleifera exhibits protective effects in mice challenged with Salmonella typhi via TLR3/TLR4 inhibition and down-regulation of proinflammatory cytokines

Wuryandari

Atho'illah

Laili

et al. 2022

Journal of Ayurveda and Integrative Medicine

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Background Salmonella typhi is a foodborne pathogenic bacterium that threatens health. S. typhi infection exacerbated the antibiotic resistance problem that needs alternative strategies. Moringa oleifera possesses anti-inflammatory and antimicrobial effects. However, there is a lack of information about the pharmacological value of red M. oleifera . The fermentation of red M. oleifera leaves extract (RMOL) is expected to add to its nutritional value. Objective The present study aimed to evaluate non-fermented RMOL (NRMOL) and fermented RMOL (FRMOL) effects on S. typhi infection in mice. Materials and methods Female Balb/C mice were randomly divided into eight groups. The treatment groups were orally administered with NRMOL or FRMOL at doses 14, 42, and 84 mg/kg BW during the 28 days experimental period. Then S. typhi was introduced to mice through intraperitoneal injection except in the healthy groups. The NRMOL or FRMOL administration was continued for the next seven days. Cells that expressed CD11b + TLR3 + , CD11b + TLR4 + , CD11b + IL-6 + , CD11b + IL-17 + , CD11b + TNF-a + , and CD4 + CD25 + CD62L + were assessed by flow cytometry. Results Our result suggested that NRMOL and FRMOL extracts significantly reduced ( p < 0.05 ) the expression of CD11b + TLR3 + , CD11b + TLR4 + , CD11b + IL-6 + , CD11b + IL-17 + , and CD11b + TNF-α + subsets. In contrast, NRMOL and FRMOL extracts significantly increased ( p < 0.05 ) the expression of CD4 + CD25 + CD62L + subsets. NRMOL at dose 14 and 42 mg/kg BW was more effective compared to FRMOL in reducing the expression of CD11b + TLR3 + , CD11b + TLR4 + , and CD11b + TNF-α + subsets. Conclusion Our findings demonstrated that NRMOL and FRMOL extracts could be promising agents for protection against S. ty...

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Single garlic oil modulates T cells activation and proinflammatory cytokine in mice with high fat diet

Cited by 24 publications

References 27 publications

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Elicited soybean extract attenuates proinflammatory cytokines expression by modulating TLR3/TLR4 activation in high−fat, high−fructose diet mice

Roles and mechanisms of garlic and its extracts on atherosclerosis: A review

Lactobacillus plantarum FNCC 0137 fermented red Moringa oleifera exhibits protective effects in mice challenged with Salmonella typhi via TLR3/TLR4 inhibition and down-regulation of proinflammatory cytokines

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