Single molecule detection and photochemistry on a surface using near-field optical excitation

Ambrose, W. P.; Goodwin, Peter M.; Martin, John; Keller, Richard A.

doi:10.1103/physrevlett.72.160

Cited by 241 publications

(167 citation statements)

References 19 publications

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“…If the isolated molecular Raman scattering is very low, it can be used to achieve the purpose of single molecule detection [170][171][172]. Although SERS has demonstrated great potential in the field of molecular detection, the difference in the surface roughness of the metal and the difference in the absorption position of molecules on the metal surface result in a nonreproducibility of the experiments.…”

Section: Sersmentioning

confidence: 99%

Exciton-plasmon coupling interactions: from principle to applications

et al. 2017

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Abstract:The interaction of exciton-plasmon coupling and the conversion of exciton-plasmon-photon have been widely investigated experimentally and theoretically. In this review, we introduce the exciton-plasmon interaction from basic principle to applications. There are two kinds of exciton-plasmon coupling, which demonstrate different optical properties. The strong exciton-plasmon coupling results in two new mixed states of light and matter separated energetically by a Rabi splitting that exhibits a characteristic anticrossing behavior of the exciton-LSP energy tuning. Compared to strong coupling, such as surface-enhanced Raman scattering, surface plasmon (SP)-enhanced absorption, enhanced fluorescence, or fluorescence quenching, there is no perturbation between wave functions; the interaction here is called the weak coupling. SP resonance (SPR) arises from the collective oscillation induced by the electromagnetic field of light and can be used for investigating the interaction between light and matter beyond the diffraction limit. The study on the interaction between SPR and exaction has drawn wide attention since its discovery not only due to its contribution in deepening and broadening the understanding of SPR but also its contribution to its application in light-emitting diodes, solar cells, low threshold laser, biomedical detection, quantum information processing, and so on.

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Section: Sersmentioning

confidence: 99%

Exciton-plasmon coupling interactions: from principle to applications

et al. 2017

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“…These include e.g., a modified version of flow cytometry [63,64 ] used for single fluorophore detection and nearfield microscopy [65][66][67], evanescent-field fluorescence microscopy (EFFM, also called total internal reflection microscopy [68]) [69], and versions of epi-fluorescence microscopy [70][71][72][73] used for single fluorophore imaging. In the case of low temperature experiments, reviewed in Ref.…”

Section: Introductionmentioning

confidence: 99%

Homebuilt single-molecule scanning confocal fluorescence microscope studies of single DNA/protein interactions

Zheng¹,

Goldner²,

Leuba³

2007

Methods

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Many technical improvements in fluorescence microscopy over the years have focused on decreasing background and increasing the signal to noise ratio (SNR). The scanning confocal fluorescence microscope (SCFM) represented a major improvement in these efforts. The SCFM acquires signal from a thin layer of a thick sample, rejecting light whose origin is not in the focal plane thereby dramatically decreasing the background signal. A second major innovation was the advent of high quantum-yield, low noise, single-photon counting detectors. The superior background rejection of SCFM combined with low-noise, high-yield detectors makes it possible to detect the fluorescence from single dye molecules. By labeling a DNA molecule or a DNA/protein complex with a donor/ acceptor dye pair, fluorescence resonance energy transfer (FRET) can be used to track conformational changes in the molecule/complex itself, on a single molecule/complex basis. In this methods paper, we describe the core concepts of SCFM in the context of a study that uses FRET to reveal conformational fluctuations in individual Holliday junction DNA molecules and nucleosomal particles. We also discuss data processing methods for SCFM.

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“…For example, bleaching of a single molecule can appear in one step 2,4 after several fluorescence cycles, the number of which may vary statistically between different molecules, whereas a fluorescence bleaching curve of a molecule ensemble decays continuously and nearly exponentially in time. Variations from a singleexponential bleaching curve may be related to the random orientation within the ensemble or to inhomogeneities in the individual local environment of each molecule.…”

Section: Introductionmentioning

confidence: 99%

“…Apart from permanent bleaching, new effects have already been observed on the singlemolecule level, such as large, photoinduced spectral shifts 12,13 and, possibly related to this, a sudden reappearance of fluorescence some time after an instantaneous drop of the fluorescence intensity. 2,4,14 One fascinating application of microscopic singlemolecule fluorescence detection is the observation and localization of chemical reactions on the single-molecule level. 15,16 To obtain insights that are statistically relevant, one must collect data from many molecules.…”

Section: Introductionmentioning

confidence: 99%

“…The microscopic observation of fluorescence from single chromophores adsorbed on surfaces or in thin films or membranes [1][2][3][4][5][6][7] and in fluids 8,9 at room temperature and low temperatures 10,11 gives direct insight into several processes that are not accessible in ensemble measurements, where fluorescence is detected from many molecules simultaneously. For example, bleaching of a single molecule can appear in one step 2,4 after several fluorescence cycles, the number of which may vary statistically between different molecules, whereas a fluorescence bleaching curve of a molecule ensemble decays continuously and nearly exponentially in time.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fluorescence Blinking and Photobleaching of Single Terrylenediimide Molecules Studied with a Confocal Microscope

Göhde¹,

Fischer²,

Fuchs³

et al. 1998

J. Phys. Chem. A

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University of Groningen A. Herrmann and K. Mu 1llenMax-Planck-Institut für Polymerforschung, D-55128 Mainz, Germany ReceiVed: April 16, 1998; In Final Form: June 9, 1998 Single terrylenediimide molecules diluted in a 20-nm-thick polyvinylbutyral polymer film were localized and observed by scanning confocal fluorescence microscopy. A modular and compact confocal microscope and the high optical stability of the molecules allowed a repeated imaging and observation over >5 h at room temperature. Most of the molecules showed several "on-off-on" transitions (blinking) on a time scale from seconds to hours, before permanent bleaching occurred. We determined that >1.5 × 10 7 fluorescence photons are emitted from the most-stable molecules before the final bleaching step occurs. Despite the "onoff-on" transitions, however, the overall change in fluorescence intensity, either integrated over each image of a time series or summed for several individual molecules, resembled an exponential-like decay, familiar from measurements of many-molecule ensembles. We also observed the polarization of the fluorescence from single molecules during excitation with circular polarized light. From these measurements, possible rotations of the molecular dipoles were studied. Over a span of 5 h, the polarization angle in most cases did not change by >15-20°. This may explain the slow and small intensity changes but excludes molecular rotation as a reason for the blinking behavior.

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Single molecule detection and photochemistry on a surface using near-field optical excitation

Cited by 241 publications

References 19 publications

Exciton-plasmon coupling interactions: from principle to applications

Exciton-plasmon coupling interactions: from principle to applications

Homebuilt single-molecule scanning confocal fluorescence microscope studies of single DNA/protein interactions

Fluorescence Blinking and Photobleaching of Single Terrylenediimide Molecules Studied with a Confocal Microscope

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