Single-Molecule DNA Amplification and Analysis Using Microfluidics

Zhang, Chunsun; Xing, Da

doi:10.1021/cr900081z

Cited by 133 publications

(98 citation statements)

References 415 publications

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“…Zhang and Xing reviewed nucleic acid amplifi cation and analysis. [ 134 ] Polymerase chain reaction (PCR) is the most widely used nucleic acid amplifi cation method in clinical and research laboratories. Many labon-chip devices implement PCR protocols.…”

Section: Preconcentration Of Analytesmentioning

confidence: 99%

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

2011

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We might be at the turning point where research in microfluidics undertaken in academia and industrial research laboratories, and substantially sponsored by public grants, may provide a range of portable and networked diagnostic devices. In this Progress Report, an overview on microfluidic devices that may become the next generation of point-of-care (POC) diagnostics is provided. First, we describe gaps and opportunities in medical diagnostics and how microfluidics can address these gaps using the example of immunodiagnostics. Next, we conceptualize how different technologies are converging into working microfluidic POC diagnostics devices. Technologies are explained from the perspective of sample interaction with components of a device. Specifically, we detail materials, surface treatment, sample processing, microfluidic elements (such as valves, pumps, and mixers), receptors, and analytes in the light of various biosensing concepts. Finally, we discuss the integration of components into accurate and reliable devices.

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Section: Preconcentration Of Analytesmentioning

confidence: 99%

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

2011

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“…Over the past decade, microfluidic LOC systems have elicited an increasing degree of interest as they can handle fast chemical reactions, require low manufacturing costs because of batch fabrication, and reduce the consumption of expensive reagents and production of waste due to precise manipulation of volumes as low as picoliters (Zhang and Xing 2010;Zhang et al, 2011).…”

Section: Microfluidic Lab-on-a-chip Systemmentioning

confidence: 99%

A mini-review on functional nucleic acids-based heavy metal ion detection

Zhan

Wang

et al. 2016

Biosensors and Bioelectronics

148

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“…The number of DNA strands in a dispensed droplet in a digital microfluidic chip has been shown to be a random variable that follows a Poisson distribution when the density of DNA strands in a sample is low [15,33]. PCR will fail when applied to a so-called "empty" droplet, which has an insufficient number of initial DNA strands [32].…”

Section: Statistical Dna Amplificationmentioning

confidence: 99%

Simulation of feedback-driven PCR assays on a 2D electrowetting array using a domain-specific high-level biological programming language

Curtis

Brisk

2015

Microelectronic Engineering

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a b s t r a c t a r t i c l e i n f oThe motivation of our work is to demonstrate that scientists can describe biochemical assays using a high-level domain-specific programming language and can execute them automatically on a two-dimensional electrowetting array featuring integrated sensors that provide feedback in real-time to the host PC that controls the integrated system. The fundamental research problem that this paper addresses is how to express, at a high level of abstraction, the acquisition of sensory information from the device, and the computation on that data, which is required to perform real-time decision making in response. The approach that we have taken is first, to create a new version of BioCoder, a programming language for automated biology, which we have specialized for electrowetting arrays featuring integrated sensors, and second, to use this language to specify two feedbackdriven Polymerase Chain Reaction (PCR) assays at the desired level of abstraction. Our methodology is to evaluate the performance of these assays using a custom-build runtime system to control the execution of feedbackdriven assays on a cycle-accurate software simulator that accurately characterizes the behavior of the electrowetting platform during assay execution. The result of this experiment is successful simulation of these non-trivial feedback-driven assays, starting from a high-level specification, which allows us to conclude that high-level programming language design and implementation targeting electrowetting (or other competing laboratory-on-a-chip technologies) is feasible.

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Single-Molecule DNA Amplification and Analysis Using Microfluidics

Cited by 133 publications

References 415 publications

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

A mini-review on functional nucleic acids-based heavy metal ion detection

Simulation of feedback-driven PCR assays on a 2D electrowetting array using a domain-specific high-level biological programming language

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