Single‐molecule Fluorescence Imaging Techniques

Abstract

The past decade has been witnessed to exciting developments in advanced fluorescence microscopy techniques that rely on visualizing single emitting fluorophores. The proliferation of single‐molecule fluorescent imaging techniques and their application in biological research have the potential to revolutionize how research is performed and greatly increase our understanding of biological systems. Presently, these techniques are still relatively niche owing to technological barriers, but it is foreseeable that they will become an increasingly common way in which insights are sought in biology. Here, we review the basic principles of key single‐molecule techniques and their recent biological applications.