Single-molecule observations for determining the orientation and diffusivity of dye molecules in lipid bilayers

Motegi, Toshinori; Nabika, Hideki; Murakoshi, Kei

doi:10.1039/c3cp51585k

Cited by 9 publications

(9 citation statements)

References 56 publications

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“…Copyright 2012, American Chemical Society. cation of a large number of nanoparticles and their specific recognition-based interactions, and lead to new applications in biosensing, bioanalysis, and chemical analysis [61] with unprecedented insights and control. Figure 5.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Dark‐Field‐Based Observation of Single‐Nanoparticle Dynamics on a Supported Lipid Bilayer for In Situ Analysis of Interacting Molecules and Nanoparticles

2014

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Observation of single plasmonic nanoparticles in reconstituted biological systems allows us to obtain snapshots of dynamic processes between molecules and nanoparticles with unprecedented spatiotemporal resolution and single-molecule/single-particle-level data acquisition. This Concept is intended to introduce nanoparticle-tethered supported lipid bilayer platforms that allow for the dynamic confinement of nanoparticles on a two-dimensional fluidic surface. The dark-field-based long-term, stable, real-time observation of freely diffusing plasmonic nanoparticles on a lipid bilayer enables one to extract a broad range of information about interparticle and molecular interactions throughout the entire reaction period. Herein, we highlight important developments in this context to provide ideas on how molecular interactions can be interpreted by monitoring dynamic behaviors and optical signals of laterally mobile nanoparticles.

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Section: Conclusion and Perspectivementioning

confidence: 99%

Dark‐Field‐Based Observation of Single‐Nanoparticle Dynamics on a Supported Lipid Bilayer for In Situ Analysis of Interacting Molecules and Nanoparticles

2014

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“…This expectation would be supported by the experimental facts that the mobility of the lipid molecule was faster in the Ld phases than in the Lo phases. [ 44 ] Moreover, there was additional line tension between Ld and Lo phases according to a previous report. [ 45 ] Therefore, the membrane flow by the EB irradiation was mainly due to the flow of the Ld phase and hardly any flow occurred in the Lo phase, which was verified by the present experimental observations that the circular shape of the Lo phase was not largely deformed during the EB irradiations (Figures 6 and 7).…”

Section: Resultsmentioning

confidence: 75%

Multi‐Scale Lipid Membrane Flow by Electron Beam‐Induced Electrowetting

Miyazako

Mabuchi

Hoshino

2021

Adv Materials Inter

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This study proposes a new technique for control of lipid membrane flow using an electron beam (EB)‐induced virtual cathode (VC). A spot 2.5 keV EB is indirectly irradiated onto supported lipid bilayers (SLBs) which are formed on a 100‐nm‐thick silicon nitride (SiN) membrane, and then a focused electric field around the EB spot (that is, the VC) causes membrane flow of the SLBs due to a voltage drop across them and electrowetting effects between them and the SiN membrane. This VC technique is shown to be able to change membrane shapes by small‐ and large‐scale controls of lipid membrane flow. For SLBs consisting of a single component, a spot VC can achieve small‐scale control of membrane flow; and the edge of the SLBs is demonstrated to change only around the spot VC. It is also shown that the spot VC can cause large‐scale deformation of SLBs consisting of ternary components by inducing a surface instability phenomenon known as Saffman–Taylor instability. The obtained results indicate that the proposed VC technique can achieve multi‐scale control of lipid membrane flow, which will contribute to on‐demand control of network topology in biomolecular devices based on artificial lipid membranes.

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“…7). [38][39][40] The observation clarified the microscopic features, such as cholesterol-induced changes in the local packing structure. Diffusion analysis gave insight into the macroscopic aspects of the phase distribution in the heterogeneous bilayer system.…”

Section: Manipulation Of a Small Number Of Molecules In Self-mentioning

confidence: 99%

“…2). [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42] The formation of a highly localized compressed phase at the gates acts as the electrochemical potential barrier for the incorporated dye-labeled lipids. The potential barrier reduces the passing ability of dye lipids through the gate, leading to the emergence of the molecular filtering phenomenon.…”

Section: Manipulation Of a Small Number Of Molecules In Self-mentioning

confidence: 99%

“…The present single-molecule orientation observation technique is useful for detailed investigative studies of the microscopic structures of heterogeneous biological membranes. 38 This cholesterol-induced change in the micro domain structure is now used to increase the potential barrier because of the compression of the bilayer membrane at the nanogate. Improvement in the separation efficiency was achieved by the introduction of cholesterol into the spreading bilayer.…”

Section: Manipulation Of a Small Number Of Molecules In Self-mentioning

confidence: 99%

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Molecule Manipulation at Electrified Interfaces using Metal Nanogates

et al. 2014

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Previously documented results on molecule manipulation using metal nanogates are comprehensively reviewed to conclude novel characteristics, which could be widely used for the developments of the systems at electrified interfaces. Novel systems to segregate molecules from self-spreading lipid bilayers in electrolyte solutions have been developed by using metal nanogate structures on solid surfaces. The spatial distribution of target molecules in the lipid bilayer changes depending on the molecule characteristics, such as charge, size, and flexibility. Energy dissipation during the spreading of the molecules on the surface contributes to the compression of the lipid bilayer at the nanogate, leading to the formation of a gradient of the electrochemical potential of the bilayer system including the target molecule in the vicinity of the nanogate (<100 nm from the gate). The gradient results in a segregation force being applied to target molecules in the order of 10 −15 N per molecule. The segregation property can be tuned by changing the electrolytes, lipid molecules, temperature, and the width and surface hydrophobicity of the metal nanogate. Introduction of asymmetry to the nanogate leads to further effective diffusion control in a direction perpendicular to the spreading. It has been demonstrated that target molecules with a different number of glycolipid per protein in the lipid bilayer are effectively separated based on the Brownian ratchet mechanism.

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Single-molecule observations for determining the orientation and diffusivity of dye molecules in lipid bilayers

Cited by 9 publications

References 56 publications

Dark‐Field‐Based Observation of Single‐Nanoparticle Dynamics on a Supported Lipid Bilayer for In Situ Analysis of Interacting Molecules and Nanoparticles

Dark‐Field‐Based Observation of Single‐Nanoparticle Dynamics on a Supported Lipid Bilayer for In Situ Analysis of Interacting Molecules and Nanoparticles

Multi‐Scale Lipid Membrane Flow by Electron Beam‐Induced Electrowetting

Molecule Manipulation at Electrified Interfaces using Metal Nanogates

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