Single-Molecule Observations of Surfactant Diffusion at the Solution−Solid Interface

Honciuc, Andrei; Harant, Adam W.; Schwartz, Daniel K.

doi:10.1021/la8007365

Cited by 63 publications

(128 citation statements)

References 29 publications

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“…Total internal reflection fluorescence microscopy (TIRFM) [41] was used in conjunction with intermolecular Förster resonance energy transfer (FRET) and alternating laser excitation, allowing direct quantitative analysis of molecular search behavior as a function of initial searcher-target distance [42,43] These search times were directly compared to theoretical predictions based on Brownian searching and intermittent desorption-mediated searching respectively [15].…”

Section: Discussionmentioning

confidence: 99%

Interfacial Molecular Searching Using Forager Dynamics

Monserud

Schwartz

2016

Phys. Rev. Lett.

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Section: Discussionmentioning

confidence: 99%

Interfacial Molecular Searching Using Forager Dynamics

Monserud

Schwartz

2016

Phys. Rev. Lett.

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“…The diffusive trajectories of individual fluorescent molecules were determined using custom algorithms implemented in Mathematica (Wolfram Research, Champaign, IL) as described in Honciuc et al (35). Any trajectory with a total residence time shorter than 0.75 s was ignored for the purpose of statistical analysis.…”

Section: Discussionmentioning

confidence: 99%

Cadherin Diffusion in Supported Lipid Bilayers Exhibits Calcium-Dependent Dynamic Heterogeneity

Cai

Shashikanth

Leckband

et al. 2016

Biophysical Journal

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Ca 2þ ions are critical to cadherin ectodomain rigidity, which is required for the activation of adhesive functions. Therefore, changes in Ca 2þ concentration, both in vivo and in vitro, can affect cadherin conformation and function. We employed single-molecule tracking to measure the diffusion of cadherin ectodomains tethered to supported lipid bilayers at varying Ca 2þ concentrations. At a relatively high Ca 2þ concentration of 2 mM, cadherin molecules exhibited a fast diffusion coefficient that was identical to that of individual lipid molecules in the bilayer (D fast z 3 mm 2 /s). At lower Ca 2þ concentrations, where cadherin molecules were less rigid, the ensemble-average cadherin diffusion coefficient was systematically smaller. Individual cadherin trajectories were temporally heterogeneous, exhibiting alternating periods of fast and slow diffusion; the periods of slow diffusion (D slow z 0.1 mm 2 /s) were more prevalent at lower Ca 2þ concentration. These observations suggested that more flexible cadherin ectodomains at lower Ca 2þ concentration alternated between upright and lying-down conformations, where the latter interacted with more lipid molecules and experienced greater viscous drag.

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“…In previous work, we demonstrated the ability of single-molecule total internal reflection fluorescence microscopy (TIRFM) to quantify the adsorption 18,19 and diffusion [19][20][21] of surfactant probes at the solid-liquid interface in an effort to understand fundamental dynamic interfacial behaviour. In this paper, we turn this approach upside down, and use the trajectories of probe molecules (BOD-IPY-labelled dodecanoic acid) to provide images based on the lateral heterogeneity of probe-surface interactions.…”

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confidence: 99%

Super-resolution surface mapping using the trajectories of molecular probes

2011

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The surface characterization of 'soft' materials presents a significant scientific challenge, particularly under 'wet' in situ conditions where a wide variety of non-covalent interactions may be relevant. Here we introduce a new chemical imaging method, mAPT (mapping using accumulated probe trajectories) that generates images of surface interactions by distributing different aspects of molecular probe trajectories into distinct locations and then combining many trajectories to generate spatial maps. The maps are super-resolution in nature, because they are accumulated from highly localized single-molecule observations. unlike other super-resolution techniques, which report only photon or point counts, our analysis generates spatial maps of physical quantities (adsorption rate, desorption probability, local surface diffusion coefficient, surface coverage/occupancy) that are directly associated with the molecular interactions between the probe molecule and the surface. We demonstrate the feasibility of this characterization using a surface patterned with various degrees of hydrophobicity.

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Single-Molecule Observations of Surfactant Diffusion at the Solution−Solid Interface

Cited by 63 publications

References 29 publications

Interfacial Molecular Searching Using Forager Dynamics

Interfacial Molecular Searching Using Forager Dynamics

Cadherin Diffusion in Supported Lipid Bilayers Exhibits Calcium-Dependent Dynamic Heterogeneity

Super-resolution surface mapping using the trajectories of molecular probes

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