Single molecule Raman spectroscopic assay to detect transgene from GM plants

Kadam, Ulhas Sopanrao; Chavhan, R. L.; Schulz, Burkhard; Irudayaraj, Joseph

doi:10.1016/j.ab.2017.06.002

Cited by 15 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are several nanotechnological strategies evolved for biomolecular detection. For example, use of gold nanoparticles (AuNPs) in the biological analysis is well known [ 42 – 47 ]. Zhao et al designed an AuNPs-based nanoprobe for Cas sensing to improve acDNA carrier to gain fluorescence yield [ 48 ].…”

Section: Nanotechnology-based Sensingmentioning

confidence: 99%

Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

et al. 2023

View full text Add to dashboard Cite

CRISPR-Cas systems have been widely used in genome editing and transcriptional regulation. Recently, CRISPR-Cas effectors are adopted for biosensor construction due to its adjustable properties, such as simplicity of design, easy operation, collateral cleavage activity, and high biocompatibility. Aptamers’ excellent sensitivity, specificity, in vitro synthesis, base-pairing, labeling, modification, and programmability has made them an attractive molecular recognition element for inclusion in CRISPR-Cas systems. Here, we review current advances in aptamer-based CRISPR-Cas sensors. We briefly discuss aptamers and the knowledge of Cas effector proteins, crRNA, reporter probes, analytes, and applications of target-specific aptamers. Next, we provide fabrication strategies, molecular binding, and detection using fluorescence, electrochemical, colorimetric, nanomaterials, Rayleigh, and Raman scattering. The application of CRISPR-Cas systems in aptamer-based sensing of a wide range of biomarkers (disease and pathogens) and toxic contaminants is growing. This review provides an update and offers novel insights into developing CRISPR-Cas-based sensors using ssDNA aptamers with high efficiency and specificity for point-of-care setting diagnostics.

show abstract

Section: Nanotechnology-based Sensingmentioning

confidence: 99%

Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

et al. 2023

View full text Add to dashboard Cite

show abstract

“…However, biosensor and chip technologies heavily rely on the immobilization of probe sequences on solid surfaces and subsequent hybridization by the target DNA. These methods are not sensitive enough and generate background signals (Kadam et al., 2017).…”

Section: Other Detection Technologiesmentioning

confidence: 99%

A review on the identification of transgenic oilseeds and oils

Pan

Xing

Zhang

et al. 2023

Journal of Food Science

View full text Add to dashboard Cite

Transgenic technology can increase the quantity and quality of vegetable oils worldwide. However, people are skeptical about the safety of transgenic oil‐bearing crops and the oils they produce. In order to protect consumers’ rights and avoid transgenic oils being adulterated or labeled as nontransgenic oils, the transgenic detection technology of oilseeds and oils needs careful consideration. This paper first summarized the current research status of transgenic technologies implemented at oil‐bearing crops. Then, an inspection process was proposed to detect a large number of samples to be the subject rapidly, and various inspection strategies for transgenic oilseeds and oils were summarized according to the process sequence. The detection indicators included oil content, fatty acid, triglyceride, tocopherol, and nucleic acid. The detection technologies involved chromatography, spectroscopy, nuclear magnetic resonance, and polymerase chain reaction. It is hoped that this article can provide crucial technical reference and support for staff engaging in the supervision of transgenic food and for researchers developing fast and efficient monitoring methods in the future.

show abstract

“…In this regard, solid plasmonic substrates based on colloidal silver or gold nanoparticles could represent a new class of nanoobjects with broad applicability in biomedicine for the development of diagnostic strategies [3,11,13,14]. It is worth mentioning SERS analysis can also provide accuracy and sensibility in the detection of some molecular changes at plant cell level [15][16][17][18]. This tremendous capacity to enhance the Raman signal of the analytes holds a great promise for a huge number of biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Developing New Diagnostic Tools Based on SERS Analysis of Filtered Salivary Samples for Oral Cancer Detection

Borșa,

Toma,

Onaciu

et al. 2023

IJMS

View full text Add to dashboard Cite

Cancer still represents one of the biggest challenges in current medical practice. Among different types of cancer, oral cancer has a huge impact on patients due to its great visibility, which is more likely to create social stigma and increased anxiety. New early diagnose methods are still needed to improve treatment efficiency and patients’ life quality. Raman/SERS (Surface Enhanced Raman Spectroscopy) spectroscopy has a unique and powerful potential for detecting specific molecules that can become priceless biomarkers in different pathologies, such as oral cancer. In this study, a batch of saliva samples obtained from a group of 17 patients with oro-maxillofacial pathologies compared with saliva samples from 18 healthy donors using the aforementioned methods were evaluated. At the same time, opiorphin, potassium thiocyanate and uric acid were evaluated as potential specific biomarkers for oro-maxillofacial pathologies using multivariate analysis. A careful examination of SERS spectra collected on saliva samples showed that the spectra are dominated by the vibrational bands of opiorphin, potassium thiocyanate and uric acid. Given the fact that all these small molecules are found in very small amounts, we filtrated all the samples to get rid of large molecules and to improve our analysis. By using solid plasmonic substrates, we were able to gain information about molecular concentration and geometry of interaction. On the other hand, the multivariate analysis of the salivary spectra contributed to developing a new detection method for oral cancer.

show abstract

Single molecule Raman spectroscopic assay to detect transgene from GM plants

Cited by 15 publications

References 29 publications

Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets

A review on the identification of transgenic oilseeds and oils

Developing New Diagnostic Tools Based on SERS Analysis of Filtered Salivary Samples for Oral Cancer Detection

Contact Info

Product

Resources

About