Single nephron glomerular filtration rate measured by linescan multiphoton microscopy compared to conventional micropuncture

Costanzo, Vincenzo; D’Apolito, Luciano; Sardella, Donato; Iervolino, Anna; Manna, Gaetano La; Capasso, Giovambattista; Frische, Sebastian; Trepiccione, Francesco

doi:10.1007/s00424-022-02686-8

Cited by 12 publications

(8 citation statements)

References 39 publications

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“…In particular, fluorescence microscopy is a milestone in the field of microscopy. This has been instrumental in elucidating cellular pathophysiology both in vitro [ 15 , 16 , 17 ] and in vivo [ 18 , 19 , 20 ], as well as in testing the efficacy of pharmacological compounds for researching genetic disorders [ 21 , 22 ]. Fluorescence microscopy was used to show images of the organs of a 14-month-old child who died from SARS-CoV-2 [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification

Brogna¹,

Costanzo

Brogna

et al. 2023

IJMS

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SARS-CoV-2, one of the human RNA viruses, is widely studied around the world. Significant efforts have been made to understand its molecular mechanisms of action and how it interacts with epithelial cells and the human microbiome since it has also been observed in gut microbiome bacteria. Many studies emphasize the importance of surface immunity and also that the mucosal system is critical in the interaction of the pathogen with the cells of the oral, nasal, pharyngeal, and intestinal epithelium. Recent studies have shown how bacteria in the human gut microbiome produce toxins capable of altering the classical mechanisms of interaction of viruses with surface cells. This paper presents a simple approach to highlight the initial behavior of a novel pathogen, SARS-CoV-2, on the human microbiome. The immunofluorescence microscopy technique can be combined with spectral counting performed at mass spectrometry of viral peptides in bacterial cultures, along with identification of the presence of D-amino acids within viral peptides in bacterial cultures and in patients’ blood. This approach makes it possible to establish the possible expression or increase of viral RNA viruses in general and SARS-CoV-2, as discussed in this study, and to determine whether or not the microbiome is involved in the pathogenetic mechanisms of the viruses. This novel combined approach can provide information more rapidly, avoiding the biases of virological diagnosis and identifying whether a virus can interact with, bind to, and infect bacteria and epithelial cells. Understanding whether some viruses have bacteriophagic behavior allows vaccine therapies to be focused either toward certain toxins produced by bacteria in the microbiome or toward finding inert or symbiotic viral mutations with the human microbiome. This new knowledge opens a scenario on a possible future vaccine: the probiotics vaccine, engineered with the right resistance to viruses that attach to both the epithelium human surface and gut microbiome bacteria.

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Section: Introductionmentioning

confidence: 99%

Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification

Brogna¹,

Costanzo

Brogna

et al. 2023

IJMS

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“…Studies performed in animals with AKI indicate that the sustained reduction in renal blood flow and GFR is a consequence of overactivation of the tubuloglomerular feedback, secondary to proximal tubular necrosis [28]. It would be interesting in future studies to evaluate the tubuloglomerular feedback, the SNGFR, and the morphology of the proximal tubular epithelium in these groups of animals, especially since SNGFR and the morphology can now be evaluated by linescan multiphoton microscopy [29]. Interestingly, we confirmed that the expression of SerpinA3K increased after AKI, specifically in tubular cells, where it was relocated to the apical membrane [21].…”

Section: Discussionmentioning

confidence: 99%

SerpinA3K Deficiency Reduces Oxidative Stress in Acute Kidney Injury

González-Soria

Soto-Valadez

Martínez‐Rojas

et al. 2023

IJMS

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We previously showed that SerpinA3K is present in urine from rats and humans with acute kidney injury (AKI) and chronic kidney disease (CKD). However, the specific role of SerpinA3K during renal pathophysiology is unknown. To begin to understand the role of SerpinA3K on AKI, SerpinA3K-deficient (KOSA3) mice were studied 24 h after inducing ischemia/reperfusion (I/R) and compared to wild type (WT) mice. Four groups were studied: WT+S, WT+IR, KOSA3+S, and KOSA3+IR. As expected, I/R increased serum creatinine and BUN, with a GFR reduction in both genotypes; however, renal dysfunction was ameliorated in the KOSA3+IR group. Interestingly, the increase in UH2O2 induced by I/R was not equally seen in the KOSA3+IR group, an effect that was associated with the preservation of antioxidant enzymes’ mRNA levels. Additionally, FOXO3 expression was initially greater in the KOSA3 than in the WT group. Moreover, the increase in BAX protein level and the decrease in Hif1a and Vegfa induced by I/R were not observed in the KOSA3+IR group, suggesting that these animals have better cellular responses to hypoxic injury. Our findings suggest that SerpinA3K is involved in the renal oxidant response, HIF1α/VEGF pathway, and cell apoptosis.

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“…Therefore, old protocols for in vivo and in vitro experiments have been progressively optimized or substituted by new ones. In other cases, a synergic combination of more approaches has been useful to elucidate many biological processes [ 91 , 92 , 93 , 94 , 95 ]. The great advancement in the fields of genomics, bioinformatics, structural biology, and immunology offered new tools to improve the efficacy of new vaccine formulations against the AMR.…”

Section: Next Generation Vaccinesmentioning

confidence: 99%

The Potential Role of Vaccines in Preventing Antimicrobial Resistance (AMR): An Update and Future Perspectives

Costanzo

Roviello²

2023

Vaccines

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In the modern era, the consumption of antibiotics represents a revolutionary weapon against several infectious diseases, contributing to the saving of millions of lives worldwide. However, the misuse of antibiotics for human and animal purposes has fueled the process of antimicrobial resistance (AMR), considered now a global emergency by the World Health Organization (WHO), which significantly increases the mortality risk and related medical costs linked to the management of bacterial diseases. The current research aiming at developing novel efficient antibiotics is very challenging, and just a few candidates have been identified so far due to the difficulties connected with AMR. Therefore, novel therapeutic or prophylactic strategies to fight AMR are urgently needed. In this scenario, vaccines constitute a promising approach that proves to be crucial in preventing pathogen spreading in primary infections and in minimizing the usage of antibiotics following secondary bacterial infections. Unfortunately, most of the vaccines developed against the main resistant pathogens are still under preclinical and clinical evaluation due to the complexity of pathogens and technical difficulties. In this review, we describe not only the main causes of AMR and the role of vaccines in reducing the burden of infectious diseases, but we also report on specific prophylactic advancements against some of the main pathogens, focusing on new strategies that aim at improving vaccine efficiency.

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Single nephron glomerular filtration rate measured by linescan multiphoton microscopy compared to conventional micropuncture

Cited by 12 publications

References 39 publications

Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification

Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification

SerpinA3K Deficiency Reduces Oxidative Stress in Acute Kidney Injury

The Potential Role of Vaccines in Preventing Antimicrobial Resistance (AMR): An Update and Future Perspectives

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