Single nucleotide polymorphism in the promoter region of the lactoferrin gene and its associations with milk performance traits in Polish Holstein-Friesian cows

Kamiński, Stanisław; Oleński, Kamil; Brym, Paweł; Malewski, Tadeusz; Sazanov, Alexei A

doi:10.1134/s1022795406080114

Cited by 25 publications

(35 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of many studies confirmed that LTF gene may be considered as QTL for milk performance traits and natural resistance to mastitis (Seyfert et al 1996, Li et al 2004, Daly et al 2006, Kamiński et al 2006, Huang et al 2010, Sender et al 2010, Bahar et al 2011, Zabolewicz et al 2012, Wojdak-Maksymiec et al 2013). …”

Section: Discussionmentioning

confidence: 64%

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Zabolewicz¹,

Barcewicz²,

Brym³

et al. 2014

Polish Journal of Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

This study analyzed the association between single nucleotide polymorphism (A/C) in position -28 located in the TATA box of LTF gene and the lactoferrin concentration in bovine milk secreted by healthy and infected udders. Out of 241, 69 cows were selected into the experimental group and were divided into 3 groups according to mean value of somatic cell count (SCC): I <180 000 cells/mL, II: 180 000 -350 000 cells/mL and III > 350 000 cells/mL. In each SCC group, three LTF genotypes: AA, AC and CC were identified by PCR-SSCP method. A total of 604 milk samples were collected monthly and lactoferrin concentration was measured by ELISA. The 1-way ANOVA within SCC groups was performed to estimate association of -28 A/C genotypes with mean lactoferrin concentration per lactation. In the group of healthy cows (<180 000 cells/mL) LTF concentration in milk cows with the AA genotype (107.58 ± 17.92 μg/mL) was significantly higher than in homozygotes CC (52.09 ± 19.01 μg/mL). Unexpectedly, in cows with elevated SCC (>350 000 cells/mL) we observed a significant opposite relationship (207.21 ± 28.50 in CC vs 115.0 ± 28.6 μg/mL in AA). We hypothesized that a promoter with allele C, which cannot be recognized as a TATA sequence is becoming more accessible for other transcription factors, which may induce alternative LTF gene expression. We assume that our results demonstrate a very interesting effect of differential gene expression depending on polymorphism in a key regulatory motif (TATA box) and also on the health status of mammary tissues.

show abstract

Section: Discussionmentioning

confidence: 64%

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Zabolewicz¹,

Barcewicz²,

Brym³

et al. 2014

Polish Journal of Veterinary Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…Telah banyak polimorfisme yang ditemukan pada gen laktoferin baik pada daerah exon, intron maupun daerah UTR Li et al, 2004;Kamiński et al, 2006). Lebih lanjut dilaporkan bahwa polimorfisme pada daerah pengaturan memiliki pengaruh pada ekspresi gen. Seyfert dan Kühn (1994) mendeteksi adanya 6 polimorfisme pada daerah UTR'5 dan 1 polimorfisme pada exon 1.…”

Section: Pendahuluanunclassified

Analisis Keragaman Gen Laktoferin Pada Sapi Friesian-Holstein Dengan Metode Pcr-Rflp

Mariana

2011

J. Agripet

View full text Add to dashboard Cite

The purposes of this study were to identify the polymorphism of the lactoferrin gene in Holstein-Friesian (HF) cows.The study was conducted on 281 heads of HF lactating cows coming from dairy farmers in Lembang district.Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65.5%) and AB (34.5%) genotypes.

show abstract

“…Telah banyak polimorfisme yang ditemukan pada gen laktoferin baik pada daerah exon, intron maupun daerah UTR Li et al, 2004;Kamiński et al, 2006). Lebih lanjut dilaporkan bahwa polimorfisme pada daerah pengaturan memiliki pengaruh pada ekspresi gen. Sequen gen laktoferin dari Gen Bank menunjukkan fragmen dimana mutasi terjadi, dimana titik mutasi tersebut dikenali oleh enzim restriksi EcoRI.…”

Section: Pendahuluanunclassified

“…Li et al (2004) menemukan 6 SNP yang lainnya yaitu: 1 pada exon 4, 1 pada intron 4, 1 pada exon 8, 2 pada exon 9 dan 1 pada exon 15. Polimorfisme pada posisi +32 (G/C) memiliki peranan penting pada determinasi kandungan dan kualitas protein susu, tetapi tidak memiliki hubungan dengan jumlah sel somatik susu (Kamiński et al, 2006). Lebih lanjut Kamiński et al (2006) menyebutkan bahwa alel G berhubungan dengan ekspresi laktoferin yang rendah dan daerah promoter berhubungan erat dengan rendahnya jumlah sel somatik susu, sebaliknya alel C berkaitan dengan peningkatan kadar laktoferin dalam susu yang menyebabkan system pertahanan tubuh yang lebih baik.…”

Section: Pendahuluanunclassified

“…Polimorfisme pada posisi +32 (G/C) memiliki peranan penting pada determinasi kandungan dan kualitas protein susu, tetapi tidak memiliki hubungan dengan jumlah sel somatik susu (Kamiński et al, 2006). Lebih lanjut Kamiński et al (2006) menyebutkan bahwa alel G berhubungan dengan ekspresi laktoferin yang rendah dan daerah promoter berhubungan erat dengan rendahnya jumlah sel somatik susu, sebaliknya alel C berkaitan dengan peningkatan kadar laktoferin dalam susu yang menyebabkan system pertahanan tubuh yang lebih baik. Laporan terbaru dari Kaminski et al (2008) menyatakan bahwa kadar protein susu yang lebih tinggi memiliki kaitan yang erat dengan polimorfisme pada posisi +216.…”

Section: Pendahuluanunclassified

See 1 more Smart Citation

Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein

Mariana

2011

J. Agripet

View full text Add to dashboard Cite

The purposes of this study were to identify the polymorphism of the lactoferrin gene and the association of genetic variants of the lactoferrin gene on milk quality in Holstein-Friesian (HF) cows. The study was conducted on 194 heads of HF lactating cows coming from semi intensive of small dairy farmers in two villages of the KPSBU Lembang, Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65%) and AB (35%) genotypes.Both the AA and AB genotypes had inconsistency in controlling component of the milk quality. Further, the AB lactoferrin cows had a higher frequency in the classification of low somatic cells count (0.75), while the AA cows were at a higher frequency in the classification of high somatic cells count (0.60). It was concluded that there is no strong relationship between intron 6 lactoferrin gene with milk quality parameters. Further, the AB genotype of the lactoferrin gene had a consistency in producing lower somatic cells count compared to the AA genotype.

show abstract

Single nucleotide polymorphism in the promoter region of the lactoferrin gene and its associations with milk performance traits in Polish Holstein-Friesian cows

Cited by 25 publications

References 14 publications

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Analisis Keragaman Gen Laktoferin Pada Sapi Friesian-Holstein Dengan Metode Pcr-Rflp

Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein

Contact Info

Product

Resources

About