Single nucleotide polymorphism of FSHβ gene associated with reproductive traits in Japanese flounder (Paralichthys olivaceus)

Abstract

Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development. This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder. We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals. We identified only an SNP (T/C) in the coding region of exon3 of FSHβ. The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene. Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P < 0.05). Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P < 0.05) than that of genotype CC. Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder.

Expression patterns of cytochrome P450 aromatase genes during ovary development and their responses to temperature stress in female yellow catfish (Pelteobagrus fulvidraco)

“…Steroid radioimmunoassay (RIA) was carried out following He et al (2010) to determine serum T and E 2 levels.…”

Mutations in exons of the CYP17-II gene affect sex steroid concentration in male Japanese flounder (Paralichthys olivaceus)

“…The single-stranded conformation polymorphism (SSCP) method was used for genotype CYP17-Ⅱ gene (He et al, 2010). Only 75 samples were used for analysis by PCR-SSCP.…”