Single Oral Dose Toxicity Test of Blue Honeysuckle Concentrate in Mice

Abstract

The objective of this study was to obtain single oral dose toxicity information for concentrated and lyophilized powder of blue honeysuckle (Lonicera caerulea L., Caprifoliaceae; BHcL) in female and male ICR mice to aid in the process of developing natural origin medicinal ingredients or foods following proximate analysis and phytochemical profile measurement. The proximate analysis revealed that BHcL had an energy value of 3.80 kcal/g and contained 0.93 g/g of carbohydrate, 0.41 g/g of sugar, 0.02 g/g of protein, and 0.20 mg/g of sodium. BHcL did not contain lipids, including saturated lipids, trans fats, or cholesterols. Further, BHcL contained 4.54% of betaine, 210.63 mg/g of total phenols, 159.30 mg/g of total flavonoids, and 133.57 mg/g of total anthocyanins. Following administration of a single oral BHcL treatment, there were no treatment-related mortalities, changes in body weight (bw) or organ weight, clinical signs, necropsy or histopathological findings up to 2,000 mg/kg bw, the limited dosage for rodents of both sexes. We concluded that BHcL is a practically non-toxic material in toxicity potency.

Effects of mixed formulation of tamoxifen and blue honeysuckle on the pharmacokinetics profiles of tamoxifen after single oral administration

“…Deep purple colored solution of concentrated blue honeysuckle, about 63 brix, were supplied by H&K Bioscience Co., Ltd. (Seoul, Korea) and used as same as our previous single oral dose toxicity test 51) in this experiment. The brief process for making BH was as follows.…”

“…Recently, orally administered blue honeysuckle was reported to protect mice against ionizing radiation 45) , ameliorates abnormal lipid and glucose metabolism in rats 46) , hepatoprotective effects 47) , anti-inflammatory effects 48) . Especially blue honeysuckle extracts have been showed the strongest antioxidant potent among 12 types of colored berries 49) , and phenolic rich extract of BH has been shown to possess anti-inflammatory and wound-healing effects in vitro and in vivo 48) and skin protective effects to ultraviolet-induced damages 50) with less toxicity 51) .…”

Cardioprotective Effects of Radix on the Pressure Overloaded Heart Failure Model by Transverse Aortic Constriction-induced Mice

“…At sacrifice, the weights of heart in all survived animals were evaluated at g levels, individually with an automatic electronic balance (Precisa Instrument, Dietikon, Switzland), and to reduce the differences from individual body weights, the relative weights (% of body weights) were also calculated using body weight at sacrifice and absolute heart weight as follow Equation [2] based on previously established methods with some modifications 12) .…”

Laxative effects of triple fermented barley extracts (FBe) on loperamide (LP)-induced constipation in rats

“…There has recently been a rise in attention towards the role of functional foods in maintaining well-being, resulting in an increased demand for functional foods produced from natural sources [8]. Natural products are gaining interest in the biopharmaceutical industry as well as inspiring the search for novel potential sources of bioactive metabolites [9, 10].…”