Single-particle-frit-based packed columns for microchip chromatographic analysis of neurotransmitters

Design Guidelines and Kinetic Performance Limits for Spatial Comprehensive Three-Dimensional Chromatography for the Analysis of Intact Proteins

“…Pareto-fronts of the total analysis time vs peak capacity for spatial 3D-LC devices with channel distances (α) ranging between 0.4 and 1.5 mm (red lines) and 0.05 and 0.4 mm (black lines). The full lines represent spatial 3D-LC devices with 3 D channels packed equally well to a commercial column (α, b , and c values in Table ), while the dashed lines stand for devices accounting for the chip packing quality of 3 D channels (α, b , and c values from ref ).…”

“…Figure 3 demonstrates that the largest gain in n c (369%) can be realized when increasing the separation space (channel length) in t RPLC as n c almost linearly increases with the length in the range applied. When considering a b c terms accounting for the difficulties encountered in packing microchannels, 15 the total peak capacity in RPLC will be reduced by approximately 30% (see Figure S1 and Section S5) and the gain will be 363%…”

“…that can be achieved with the current state-of-the-art micromilling and solvent-vapor-assisted-bonding technology, as utilized for the fabrication of spatial 2D-LC and 3D-LC microchips in previous studies performed by our group. 2,16,17 The red dashed line in Figure 5 shows the Pareto front, considering the effect of the packing quality on the resulting performance (as reported for microchips 15 ). For all devices, the peak capacity increases almost linearly with the analysis time.…”

Microfluidic devices for protein analysis using intact and top‐down mass spectrometry

“…TD-MS microchips for TD proteomics primarily adopt these two principles for separating various proteins. Three types of LC columns, that is, opentube, 74,75 packed bed, [76][77][78] and monolithic bed 79,80 have been employed for LC separation. Chen et al integrated an 8-cm-long C18 LC column in a silicon multi-nozzle emitter array (MEA) microchip with an internal swept volume of < 15 nl, and well separated intact human plasma proteins including human serum albumin (HSA), hemoglobin A (HbA), and apolipoprotein A-I (apoA-I).…”

“…TD‐MS microchips for TD proteomics primarily adopt these two principles for separating various proteins. Three types of LC columns, that is, open‐tube, 74,75 packed bed, 76–78 and monolithic bed 79,80 have been employed for LC separation. Chen et al.…”