2022
DOI: 10.1002/advs.202203018
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Single Photon Kilohertz Frame Rate Imaging of Neural Activity

Abstract: Establishing the biological basis of cognition and its disorders will require high precision spatiotemporal measurements of neural activity. Recently developed genetically encoded voltage indicators (GEVIs) report both spiking and subthreshold activity of identified neurons. However, maximally capitalizing on the potential of GEVIs will require imaging at millisecond time scales, which remains challenging with standard camera systems. Here, application of single photon avalanche diode (SPAD) sensors is reporte… Show more

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Cited by 4 publications
(3 citation statements)
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“…46,48,49 The use of modern GEVIs in combination with modern microscopic technologies makes it possible to combine subcellular spatial resolution with millisecond resolution from identified neuronal populations in living brain slices. 50,51 For transient expression, indicators can be injected directly into the animal's brain using lentiviral or adenovirus vectors. An alternative way is to obtain transgenic animals expressing these indicators under the control of tissue-specific or constitutive promoters.…”
Section: Genetically Encoded Indicators Of Neural Activitymentioning
confidence: 99%
“…46,48,49 The use of modern GEVIs in combination with modern microscopic technologies makes it possible to combine subcellular spatial resolution with millisecond resolution from identified neuronal populations in living brain slices. 50,51 For transient expression, indicators can be injected directly into the animal's brain using lentiviral or adenovirus vectors. An alternative way is to obtain transgenic animals expressing these indicators under the control of tissue-specific or constitutive promoters.…”
Section: Genetically Encoded Indicators Of Neural Activitymentioning
confidence: 99%
“…The integration of ultra-high-speed cameras 7,8,21 and event cameras 22 holds promise for providing higher bandwidths to LFM. However, their current limitations in sensitivity and noise performance present challenges, especially for photon-starved applications like imaging genetically encoded voltage indicators (GEVIs).…”
Section: Introductionmentioning
confidence: 99%
“…Some fruitful methods have been studied to suppress fluorescent noise, for example, using total internal reflection illumination, spinning disk confocal illumination, and light sheet microscopy. , The detector noises accompany each acquisition process, which are much less discussed. They are mainly determined by the performance of the detectors [charge-coupled devices (CCD) or complementary metal-oxide semiconductor (CMOS)]. , For SMLM with nanometer-level localization precision, especially under weak-signal conditions, detector noises can cause localization misalignments and signal losses, so their removal is indispensable. However, due to the complexity of the detector noises, the multi-frame accumulate and average (ANA) , commonly used in experiments cannot efficiently eliminate these noises; therefore, the fluorescent signal extraction becomes less effective.…”
Section: Introductionmentioning
confidence: 99%