Single radial haemolysis compared to haemagglutinin inhibition and microneutralization as a correlate of protection against influenza A H3N2 in children and adolescents

Wang, Biao; Russell, Margaret L.; Brewer, Angela; Newton, Jennifer; Singh, Pardeep; Ward, Brian J.; Loeb, Mark

doi:10.1111/irv.12450

Cited by 18 publications

(24 citation statements)

References 17 publications

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“…Previous studies involving A strains support the strong agreement between HI and SRH assays found in present study . The correlation between SRH and MN assays observed in this study is in agreement with the findings of the previous studies that used equine influenza strains and H3N2 influenza strain . However, unlike Wang et al , who found much higher correlations between SRH and MN assays than between SRH‐HI and HI‐MN assays, we found no significant differences among them.…”

Section: Discussionsupporting

confidence: 92%

“…The data highlight strong correlations between HI, MN, and SRH assays for influenza A strains (H1N1 and H3N2). A significant correlation between HI and MN has been found by Veguilla et al with the same H1N1 strain used in this study, and, in other studies, with different influenza strains, such as A/Brisbane/10/2007 (H3N2), A/Brisbane/59/2007 (H1N1), and equine influenza viruses . Previous studies involving A strains support the strong agreement between HI and SRH assays found in present study .…”

Section: Discussionsupporting

confidence: 91%

“…11,14,28 The correlation between SRH and MN assays observed in this study is in agreement with the findings of the previous studies that used equine influenza strains and H3N2 influenza strain. 11,29,30 However, unlike Wang et al, 30 who found much higher correlations between SRH and MN assays than between SRH-HI and HI-MN assays, we found no significant differences among them. The correlation among the three assays was also evaluated for influenza B strains (Victoria and Yamagata lineages), showing good agreement.…”

Section: F I G U R E 1 Continuedcontrasting

confidence: 99%

“…In addition, the assay agreement on protection, as defined by 11,29,30,33 Previous studies involving A strains support the strong agreement between HI and SRH assays found in present study. 11,14,28 The correlation between SRH and MN assays observed in this study is in agreement with the findings of the previous studies that used equine influenza strains and H3N2 influenza strain.…”

Section: F I G U R E 1 Continuedsupporting

confidence: 90%

“…11,14,[27][28][29] A recent study has revealed that the correlation between SRH and MN is greater than that between SRH-HI and HI-MN assays. 30 ELISA shows good agreement between the HI and MN assays; notably, ELISA seems to be more sensitive than HI assay, especially in detecting low levels of antibody, owing to the low background. 24,31,32 Few previous studies have dealt with the correlations among the four assays.…”

mentioning

confidence: 87%

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Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

Trombetta

Remarque

Mortier

et al. 2018

Influenza Resp Viruses

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BackgroundThe immunological response to influenza vaccine and/or natural infection is evaluated by serological techniques, the most common being hemagglutination inhibition (HI), single radial hemolysis (SRH), and virus neutralization assays, which is commonly used in a micro‐neutralization (MN) format. ELISA is not officially required; however, this assay is able to measure different class‐specific antibodies. The four assays identify different sets or subsets of antibodies.ObjectivesThe aim of this study was to establish the correlation among four serological assays using four seasonal influenza strains.MethodsThe HI, SRH, MN assays, and ELISA were performed on four seasonal influenza strains.ResultsA strong positive correlation was found between HI and MN and between SRH and MN assays for influenza A strains. The B strains also showed good correlations among the three assays. A positive correlation was also found between ELISA and the “classical” assays for all strains. Concerning the correlates of protection, as defined by HI ≥ 40 and SRH ≥ 25 mm2, good agreement was observed for the influenza A strains. By contrast, the agreement for the B strains was very low.ConclusionsThere is a positive strong correlation among the four serological assays for both A and B strains, especially for the HI and MN assays. There is good agreement on correlates of protection between HI and SRH assays for the A strains, but very low agreement for the B strains, suggesting higher sensitivity of SRH than HI assay in detecting antibodies against the influenza B viruses.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: F I G U R E 1 Continuedcontrasting

confidence: 99%

Section: F I G U R E 1 Continuedsupporting

confidence: 90%

mentioning

confidence: 87%

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Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

Trombetta

Remarque

Mortier

et al. 2018

Influenza Resp Viruses

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A changing trend in diagnostic methods of Influenza A (H3N2) virus in human: a review

et al. 2021

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The influenza virus is classified into four types A, B, C, and D, but type A and B are responsible for major illnesses in people with influenza A being the only virus responsible for flu pandemics due to the presence of two surface proteins called hemagglutinin (H) and neuraminidase (N) on the virus. The two subtypes of influenza A virus, H1N1 and H3N2, have been known to cause many flu pandemics. Both subtypes change genetically and antigenically to produce variants (clades and subclades, also know as groups and subgroups). H3N2 tends to change rapidly, both genetically and antigenically whereas that of H1N1 generally tends to have smaller changes. Influenza A (H3N2) viruses have evolved to form many separate, genetically different clades that continue to co-circulate. Influenza A(H3N2) viruses have caused significant deaths as per WHO report. The review describes methods for detection of influenza A(H3N2) viruses by conventional serological methods as well as the advanced methods of molecular biology and biosensors. All these methods are based on different parameters and have different targets but the goal is to improve specificity and increase sensitivity. Amongst the molecular methods, real-time polymerase chain reaction (RT-PCR) is considered a gold standard test due to its many advantages whereas a number of other molecular methods are time-consuming, complex to perform or lack specificity. The review also considers bio-sensing methods for simple, rapid, highly sensitive, and specific detection of H3N2. The classification and principle of various H3N2 biosensors are also discussed.

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Influenza A haemagglutinin specific IgG responses in children and adults after seasonal trivalent live attenuated influenza vaccination

Islam

Mohn

Krammer

et al. 2017

Vaccine

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Influenza is a major respiratory pathogen and vaccination is the main method of prophylaxis. In 2012, the trivalent live attenuated influenza vaccine (LAIV3) was licensed in Europe for use in children. Vaccine-induced antibodies directed against the main viral surface glycoprotein, haemagglutinin (HA), play an important role in virus neutralization through different mechanism. The objective of this study was to dissect the HA specific antibody responses induced after LAIV3 immunization to the influenza A viruses in children and adults. Plasma was collected from 20 children and 20 adults pre- and post-LAIV3 vaccination (up to ayear) and analysed by the haemagglutination inhibition (HI) and ELISA assays. We found that LAIV3 boosted the HA specific IgG response against the head and the full-length of H3N2 in children, but not adults. Adults had higher levels of pre-existing stalk antibodies (towards H3N2 and H1N1), but these were not boosted by LAIV3. Importantly, we observed a trend in boosting of H1N1 HA stalk specific antibodies in children after LAIV3. Whereas, heterosubtypic H5 and H7 full-length HA specific antibodies were not boosted in either children or adults. In conclusion, LAIV3 elicited H3-head and low levels of H1 stalk specific antibody responses in children, supporting the prophylactic use of LAIV in children.

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Single radial haemolysis compared to haemagglutinin inhibition and microneutralization as a correlate of protection against influenza A H3N2 in children and adolescents

Cited by 18 publications

References 17 publications

Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

Comparison of hemagglutination inhibition, single radial hemolysis, virus neutralization assays, and ELISA to detect antibody levels against seasonal influenza viruses

A changing trend in diagnostic methods of Influenza A (H3N2) virus in human: a review

Influenza A haemagglutinin specific IgG responses in children and adults after seasonal trivalent live attenuated influenza vaccination

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