Single-shot compressed ultrahigh-speed imaging for fast wide-field upconversion luminescence lifetime thermometry

Abstract

We develop an optical temperature mapping modality, termed single-shot photoluminescence lifetime imaging thermometry (SPLIT). Synergistically combining dual-view optical streak imaging with compressed sensing, SPLIT records wide-field luminescence decay of Er 3+ , Yb 3+ co-doped NaGdF4 upconverting nanoparticles in real time, from which a lifetime-based 2D temperature map is obtained in a single exposure. Largely advancing existing optical thermometry techniques in detection capabilities, SPLIT enables longitudinal 2D temperature monitoring beneath a thin scattering medium and dynamic temperature tracking of a moving biological sample at single-cell resolution. SPLIT will be applied to early melanoma diagnosis and extended to the near-infrared spectral region.