In this research, it was aim to investigate the clothing comfort properties of knitted fabrics intended for summer cycling sportswear. Five different fiber types and five different knitted structures commonly used in cycling sportwear were selected and totally 25 fabrics were manufactured by using a seamless circular knitting machine namely Santoni SM8-TOP2V in a controlled manner. To assess the impact of material type and knitted structures on clothing comfort properties, mass per unit area, thickness, air permeability, thermal conductivity, thermal resistance, overall moisture management capability and relative vapour permeability tests were conducted. The statistical significance of variations in the obtained results was evaluated using the analysis of variance (ANOVA) method. In conclusion, the most suitable material and knitted structure have been recommended for summer upper body cycling clothing.