Single spore isolation and interspecific hybridization of Calocybe spp.

Abstract

For the development of improved strain(s) of Calocybe spp. with desirable traits, interspecific hybridization of Milky white mushroom (C. indica) and St. George's mushroom (C. gambosa) was done by intermating their single spore cultures. In vitro evaluation of both the species for mycelial characteristics, growth rate, colony colour, days taken for completion of full growth were recorded. Single spore isolates of the two species were developed through serial dilution technique. Hybridisation of single spores was done by dual culture technique using hyphal anastomosis. Of the 121 crosses done 42 were compatible and 79 crosses incompatible.