Single-step noncompetitive immunocomplex immunoassay for rapid aflatoxin detection

Peltomaa, Riikka; Abbas, Asmaa; Yli-Mattila, Tapani; Lamminmäki, Urpo

doi:10.1016/j.foodchem.2022.133287

Cited by 18 publications

(6 citation statements)

References 28 publications

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“…Similarly, a competitive magnetic immunodetection assay was implemented by Pietschmann and colleagues (2020) for the detection and quantification of AFB1, but it has a detection limit of 1.1 ng/mL [47]. Recently, Peltomaa and colleagues (2022) developed a single-step immunoassay based on a monoclonal capture antibody and a recombinant anti-immunocomplex antibody fragment isolated from a synthetic antibody repertoire to detect AF in contaminated food samples [48]. This assay has a detection limit of 70 pg/mL and produces results within 15 min, utilizing a single incubation step where all three antibody reagents (biotinylated monoclonal antibody, anti-IC binder, and europium-labeled secondary antibody) are added simultaneously.…”

Section: Immunochemical Methodsmentioning

confidence: 99%

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

Alameri

Kong

Aljaafari

et al. 2023

Toxins

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Aflatoxins (AFs) represent one of the main mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus, with the most prevalent and lethal subtypes being AFB1, AFB2, AFG1, and AFG2. AFs are responsible for causing significant public health issues and economic concerns that affect consumers and farmers globally. Chronic exposure to AFs has been linked to liver cancer, oxidative stress, and fetal growth abnormalities among other health-related risks. Although there are various technologies, such as physical, chemical, and biological controls that have been employed to alleviate the toxic effects of AF, there is still no clearly elucidated universal method available to reduce AF levels in food and feed; the only mitigation is early detection of the toxin in the management of AF contamination. Numerous detection methods, including cultures, molecular techniques, immunochemical, electrochemical immunosensor, chromatographic, and spectroscopic means, are used to determine AF contamination in agricultural products. Recent research has shown that incorporating crops with higher resistance, such as sorghum, into animal feed can reduce the risk of AF contamination in milk and cheese. This review provides a current overview of the health-related risks of chronic dietary AF exposure, recent detection techniques, and management strategies to guide future researchers in developing better detection and management strategies for this toxin.

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Section: Immunochemical Methodsmentioning

confidence: 99%

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

Alameri

Kong

Aljaafari

et al. 2023

Toxins

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show abstract

“…A single-step assay has been developed for the rapid detection of AFB1 in maize, rice, and hazelnut within 15 min with a LOD of 70 pg/mL. For this method, anti-immunocomplex (anti-IC) antibodies were used and the immunoassay was non-competitive showing the applicability of these parameters in the analysis of small molecule contaminants [ 110 ]. Furthermore, a chromatography-free method was found in the literature for the detection of AFB1 in cereals and oils through atomic absorption spectroscopy (AAS) using quantum dots and immunomagnetic beads.…”

Section: Instrumental Analysismentioning

confidence: 99%

Recent Advances in Mycotoxin Determination in Fish Feed Ingredients

et al. 2023

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Low-cost plant-based sources used in aquaculture diets are prone to the occurrence of animal feed contaminants, which may in certain conditions affect the quality and safety of aquafeeds. Mycotoxins, a toxic group of small organic molecules produced by fungi, comprise a frequently occurring plant-based feed contaminant in aquafeeds. Mycotoxin contamination can potentially cause significant mortality, reduced productivity, and higher disease susceptibility; thus, its timely detection is crucial to the aquaculture industry. The present review summarizes the methodological advances, developed mainly during the past decade, related to mycotoxin detection in aquafeed ingredients, namely analytical, chromatographic, and immunological methodologies, as well as the use of biosensors and spectroscopic methods which are becoming more prevalent. Rapid and accurate mycotoxin detection is and will continue to be crucial to the food industry, animal production, and the environment, resulting in further improvements and developments in mycotoxin detection techniques.

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“…The magnetic nanoparticle-based fluorescent immunoassay provided a limit of detection for aflatoxin M1 2.9 pg/mL and a linear calibration range of 3.0 to 100 pg/mL. An immunosensor for aflatoxins was also developed in the work of Peltomaa et al [ 95 ]. They developed a non-competitive immunoassay in which a primary anti-aflatoxin antibody was bound via streptavidin to magnetic beads, and an immunocomplex was formed in the presence of aflatoxin B1 with a secondary Eu-labeled antibody.…”

Section: Progress On Immunosensors For Toxic Biological Warfare Agent...mentioning

confidence: 99%

Immunosensors for Assay of Toxic Biological Warfare Agents

Pohanka

2023

Biosensors

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An immunosensor for the assay of toxic biological warfare agents is a biosensor suitable for detecting hazardous substances such as aflatoxin, botulinum toxin, ricin, Shiga toxin, and others. The application of immunosensors is used in outdoor assays, point-of-care tests, as a spare method for more expensive devices, and even in the laboratory as a standard analytical method. Some immunosensors, such as automated flow-through analyzers or lateral flow tests, have been successfully commercialized as tools for toxins assay, but the research is ongoing. New devices are being developed, and the use of advanced materials and assay techniques make immunosensors highly competitive analytical devices in the field of toxic biological warfare agents assay. This review summarizes facts about current applications and new trends of immunosensors regarding recent papers in this area.

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Single-step noncompetitive immunocomplex immunoassay for rapid aflatoxin detection

Cited by 18 publications

References 28 publications

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

Recent Advances in Mycotoxin Determination in Fish Feed Ingredients

Immunosensors for Assay of Toxic Biological Warfare Agents

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