Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus

Paiva, Byanca Regina de; Passos, Luciana Neves; Falqueto, Aloísio; Malafronte, Rosely dos Santos; Andrade, Heitor Franco de

doi:10.1590/s0036-46652004000600007

Cited by 17 publications

(8 citation statements)

References 12 publications

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“…Foi nas primeiras décadas do século 20 que a fauna de flebotomíneos começou a ser estudada por Lutz, Barretto e Coutinho, entre outros pesquisadores, ampliaram o conhecimento sobre a biologia, a epidemiologia e principalmente a taxonomia com a descrição de diversas novas espécies. A re-emergência da LC (Camargo-Neves et al 2002) e emergência da LV (Galimbertti et al 1999) no Estado de São Paulo trouxe um novo interesse nos estudos dos flebotomíneos, e técnicas moleculares passaram a fazer parte dos estudos epidemiológicos (Rangel & Lainson 2003, Paiva et al 2004.…”

Section: Principais Lacunas Do Conhecimentounclassified

Lista de espécies de Phlebotominae (Diptera, Psychodidae) do Estado de São Paulo, Brasil, com comentários sobre sua distribuição geográfica

Shimabukuro

Galati

2011

Biota Neotrop.

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of Phlebotominae (Diptera, Psychodidae) from São Paulo State, Brazil, with notes on their geographical distribution. Biota Neotrop 11(1a): http://www. biotaneotropica.org.br/v11n1a/en/abstract?inventory+bn0361101a2011.Abstract: Phlebotomine sand flies are medically important insects, responsible for the transmission of Leishmania parasites between humans and non-human animal reservoirs, which are found throughout São Paulo State, Brazil. The 69 recorded species of phlebotomine sand flies from São Paulo State, including 7 species reported here for the first time to occur in this region, are organized in a checklist using the phylogenetic classification of Galati (2003). Our checklist incorporates and updates those previously published by and , and includes records for 33 additional phlebotomine species taken from the literature published since and our examination of specimens held in entomological museum collections. For each sand fly species, the geographical distribution by municipality is also given, together with comments on the distribution of the six vectors of cutaneous leishmaniasis, as well as Lutzomyia longipalpis, the main vector of visceral leishmaniasis.

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Section: Principais Lacunas Do Conhecimentounclassified

Lista de espécies de Phlebotominae (Diptera, Psychodidae) do Estado de São Paulo, Brasil, com comentários sobre sua distribuição geográfica

Shimabukuro

Galati

2011

Biota Neotrop.

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“…In recent years, molecular investigations have been conducted based on the use of polymerase chain reaction (PCR) (Paiva et al, 2004), for which an important initial reference is the genome sequence of the microorganism being investigated. The technique is used for diagnosis in humans (Silva et al, 2002), canines , and phlebotomines (Aransay et al, 2000;Ramalho-Ortigão et al, 2001;Miranda et al, 2002;Michalsky et al, 2002;Paiva et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Detection of Leishmania DNA in phlebotomines captured in Campo Grande, Mato Grosso do Sul, Brazil

Silva

Andreotti

Dias

et al. 2008

Experimental Parasitology

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Over the past years, leishmaniases have become a public health issue in the Brazilian state of Mato Grosso do Sul, particularly in Campo Grande, the state capital. The purpose of this study was to detect the presence of Leishmania DNA in the population of phlebotomine sandflies using DNA amplification by PCR. Insect captures were carried out from 4 pm. to 7 am for 4 consecutive days each month from October 2005 to September 2006 in 16 neighborhoods located in 7 urban regions of Campo Grande. Traps were placed indoors and in the vicinity of households. As many as 971 males and 203 females were collected. One hundred and five naturally fed females were identified and grouped as 1- to 4-specimen pools. DNA extraction was carried out using whole insects. Lutzomyia longipalpis predominated, accounting for 99.15% of the phlebotomines captured. Also found was Nyssomyia whitmani, the vector of tegumentary leishmaniasis. Abundance was greatest in the vicinity of households (69.8% of the phlebotomines captured). As revealed by PCR, parasites were present in 1.9% of the Leishmania spp. specimens investigated and confirmed for visceral leishmaniasis.

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“…Nucleic acid detection techniques in samples from people and/or animals infected with Leishmania, such as PCR, are used for detection and identi cation of the parasite since the 1980's. PCR include ampli cation of fragments of the gene encoding the small ribosomal RNA subunit (SSU rDNA,(van Eys et al, 1992), the transcribed internal ribosomal DNA spacer (ITS) (Schonian et al, 2003), sequences corresponding to kinetoplast (kDNA) (Cortes et al, 2004), mini-exon (Paiva et al, 2004), the gene encoding the heat shock protein HSP70, among others (da Silva et al, 2010). In spite of the high sensitivity of PCR and, depending on the molecular target, high speci city, it is more used in epidemiological studies than as a routine diagnostic method, and the gold standard method to diagnose Leishmania is the observation of the parasite by microscopic analysis (Thakur, Joshi, and Kaur, 2020).…”

Section: Discussionmentioning

confidence: 99%

Evolutive History of Leishmania Genus and Differential Diagnosis of Clinical Important Species Based on a Unique Kinetoplastida Chitinase

Jordão

Cabral

Pereira

et al. 2021

Int J Med Parasitol Epidemiol Sci

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Introduction: Leishmaniasis, a neglected infectious disease affecting humans, domestic, and wild animals, is caused by 20 from 53 Leishmania genus species and transmitted by sandflies. Leishmania genus, belonging to the Trypanosomatidae family and Kinetoplastida order, are grouped into five subgroups according to the biogeographic and evolutive history of parasites and hosts, leading to incongruences and paraphyly. The GH18 Leishmania chitinase, which is encoded by a species-specific single-copy gene, conserved in the basal groups of trypanosomatids, and absent in the genus Trypanosoma, was evaluated as a phylogenetic marker and a diagnostic target. Methods: Primers were designed to detect Leishmania in its host biological samples and obtain the chitinase sequence of species that are unavailable in public databanks. The GH18 chitinase gene and its genomic context were evaluated phylogenetically. A protocol was developed to discriminate Leishmania subgenera by adopting polymerase chain reaction (PCR) and restriction fragment length polymorphism and using in silico tools. The adopted PCR method for detecting a partial 953 bp GH18 chitinase-encoding gene represented high sensibility and specificity on DNA of isolated parasites and was used as negative controls, Trypanosoma cruzi, and DNA from Leishmania hosts. Results: Preservation of the chitinase locus in the aquatic free-living protozoan Bodosaltans disclosed a primitive common origin. Based on the comparative analysis, the amino acid sequence of GH18 trypanosomatidae chitinase demonstrated its high similarity to that of chitinase from marine prokaryotes and protozoans. Phylogenetic reconstruction based on chitinase corroborated the Supercontinent Origins Theory for Leishmania. Conclusion: The chitinase-encoding gene was effectively detected in biological samples and thus could be considered for differential molecular diagnosis among Leishmania clinical important species worldwide.

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Single step polymerase chain reaction (PCR) for the diagnosis of the Leishmania (Viannia) subgenus

Cited by 17 publications

References 12 publications

Lista de espécies de Phlebotominae (Diptera, Psychodidae) do Estado de São Paulo, Brasil, com comentários sobre sua distribuição geográfica

Lista de espécies de Phlebotominae (Diptera, Psychodidae) do Estado de São Paulo, Brasil, com comentários sobre sua distribuição geográfica

Detection of Leishmania DNA in phlebotomines captured in Campo Grande, Mato Grosso do Sul, Brazil

Evolutive History of Leishmania Genus and Differential Diagnosis of Clinical Important Species Based on a Unique Kinetoplastida Chitinase

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