Single‐Walled Carbon Nanotubes as Fluorescence Biosensors for Pathogen Recognition in Water Systems

Abstract

The possibility of using single-walled carbon nanotubes (SWCNTs) aggregates as fluorescence sensors for pathogen recognition in drinking water treatment applications has been studied. Batch adsorption study is conducted to adsorb large concentrations of Staphylococcus aureus aureus SH 1000 and Escherichia coli pKV-11 on single-walled carbon nanotubes. Subsequently the immobilized bacteria are detected with confocal microscopy by coating the nanotubes with fluorescence emitting antibodies. The Freundlich adsorption equilibrium constant (k) for S.aureus and E.coli determined from batch adsorption study was found to be 9 × 10 8 and 2 × 10 8 ml/g, respectively. The visualization of bacterial cells adsorbed on fluorescently modified carbon nanotubes is also clearly seen. The results indicate that hydrophobic single-walled carbon nanotubes have excellent bacterial adsorption capacity and fluorescent detection capability. This is an important advancement in designing fluorescence biosensors for pathogen recognition in water systems.

A Review of Removal of Pollutants from Water/Wastewater Using Different Types of Nanomaterials

“…CNTs (one of nanosorbents) which have been used for removal of biological impurities have received special attention for their excellent capabilities of removing biological contaminants from water [63]. CNTs possess antimicrobial characteristics against a wide range of microorganisms including bacteria such as E. coli and Salmonella [217][218][219][220][221][222] and viruses [223,224]. The adsorption of cyanobacterial toxins on CNTs is also higher when compared with carbon-based adsorbents mainly due to large specific surface area, external diameter of CNTs, large composition of mesoporous volume, and so forth [225][226][227][228].…”

Nanometrología: Impacto en los sistemas de producción

“…Por otro lado, desarrollan la metrología química para la fabricación de un catalizador para la producción de películas de nanotubos, donde hacen alusión a la importancia de "la metrología cuantitativa para la optimización del proceso" [37]. Venkata y colaboradores reportan el uso de nanotubos como sensores de fluorescencia para la detección de patógenos en el tratamiento de aguas, dada sus excelentes propiedades de adsorción [39]. Li y su equipo de trabajo reportan la fabricación de nanotubos a través de la ablación con láser, donde el diámetro es controlado por el tamaño de partícula, y la longitud de la fibra es controlada por el tiempo de deposición del vapor de carbono [40], lo que evidencian la gran aplicación de estos nanomateriales en diversas industrias.…”

Electrochemical Detection of Copper Using a Gly-Gly-His Modified Carbon Nanotube Biosensor

“…Within this field there has been significant emphasis on the development of gas sensors [26,27] or biosensors [28,29] aimed at medical applications. However, the design of carbon nanotube based sensors for aqueous detection is a relatively recent effort [30][31][32][33][34][35][36]. Much of this recent research has involved the preparation of complex composite materials.…”