Single-Walled Carbon Nanotubes Induce Cell Death and Transcription of TNF-α in Macrophages Without Affecting Nitric Oxide Production

Kim, Kyong Hoon; Yeon, Seung Min; Kim, Hyun Gyung; Lee, Hwanbum; Kim, Sun Kyung; Han, Seung Hyun; Min, Kyung Jin; Byun, Youngjoo; Lee, Eun Hee; Lee, Kenneth Sung; Yuk, Soon Hong; Ha, Un-Hwan; Jung, Yong Woo

doi:10.1007/s10753-013-9710-3

Cited by 9 publications

(5 citation statements)

References 36 publications

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“…These data are particularly interesting, since they corroborate other studies that have already shown the induction of cell death processes in different model systems exposed to CNFs (either in vitro or in vivo ). This finding is indicative of nanomaterials activating apoptotic and necrotic pathways through different pathways (Bottini et al, 2006; Elgrabli et al, 2008; Ravichandran et al, 2009; Patlolla et al, 2010; Srivastava et al, 2011; Wang et al, 2012; Kim et al, 2014; Salehcheh et al, 2020). Furthermore, data in our study also suggested that the increased rate of apoptotic and necrotic erythrocytes may have happened because of damages to cell membranes caused by direct contact of these models with CNFs or by increased oxidative stress, which was inferred through different biomarkers (H2O2, ROS and TBARS).…”

Section: Discussionmentioning

confidence: 95%

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Guimarães

Estrela

Rodrigues

et al. 2021

Preprint

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Although carbon nanotubes (CNTs) toxicity in different experimental systems (in vivo and in vitro) is known, little is known about the toxic effects of carbon nanofibers (CNFs) on aquatic vertebrates. We herein investigated the potential impact of CNFs (1 and 10 mg/L) by using Physalaemus cuvieri tadpoles as experimental model. CNFs were able to induce nutritional deficit in animals after 48-h exposure to them, and this finding was inferred by reductions observed in body concentrations of total soluble carbohydrates, total proteins, and triglycerides. The increased production of hydrogen peroxide, reactive oxygen species and thiobarbituric acid reactive substances in tadpoles exposed to CNFs has suggested REDOX homeostasis change into oxidative stress. This process was correlated to the largest number of apoptotic and necrotic cells in the blood of these animals. On the other hand, the increased superoxide dismutase and catalase activity has suggested that the antioxidant system of animals exposed to CNFs was not enough to maintain REDOX balance. In addition, CNFs induced increase in acetylcholinesterase and butyrylcholinesterase activity, as well as changes in the number of neuromats evaluated on body surface (which is indicative of the neurotoxic effect of nanomaterials on the assessed model system). To the best of our knowledge, this is the first report on the impact of CNFs on amphibians; therefore, it broadened our understanding about ecotoxicological risks associated with their dispersion in freshwater ecosystems and possible contribution to the decline in the populations of anurofauna species.

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Section: Discussionmentioning

confidence: 95%

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Guimarães

Estrela

Rodrigues

et al. 2021

Preprint

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“…Carbon nanomaterials, including SWCNTs, have been shown to modulate cellular responses such as reactive oxygen species production, 47 mitochondrial disfunction, 48 autophagy, 49 apoptosis, 50 necrosis, 51 pyroptosis, 52 etc . The formation of autophagosomes and autolysosomes is a reaction of the cell to degrade and eliminate foreign nanomaterials that have invaded the cytoplasm.…”

Section: Resultsmentioning

confidence: 99%

Specificity of carbon nanotube accumulation and distribution in cancer cells revealed by K-means clustering and principal component analysis of Raman spectra

Golubewa,

Timoshchenko,

Kulahava

2024

Analyst

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“…We previously demonstrated that CNT could induce necrosis in macrophages ( 12 ). This is probably due to the asbestosis caused by “frustrated macrophages” ( 19 ).…”

Section: Discussionmentioning

confidence: 99%

“…Using this system, we previously reported that single-walled carbon nanotubes (SWCNTs) induce cell death and tumor necrosis factor α production in macrophages without affecting NO production ( 12 ). Since carbon nanotubes (CNTs) are free floating materials, we hypothesized that CNTs-attached PVDF do not affect macrophage function.…”

Section: Introductionmentioning

confidence: 99%

Polyvinylidene Fluoride Alters Inflammatory Responses by Activation-induced Cell Death in Macrophages

Kim

et al. 2017

Immune Netw

Self Cite

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Carbon nanotubes (CNTs) are nanomaterials that have been employed in generating diverse materials. We previously reported that CNTs induce cell death in macrophages, possibly via asbestosis. Therefore, we generated CNT-attached polyvinylidene fluoride (PVDF), which is an established polymer in membrane technology, and then examined whether CNT-attached PVDF is immunologically safe for medical purposes compared to CNT alone. To test this, we treated RAW 264.7 murine macrophages (RAW cells) with CNT-attached PVDF and analyzed the production of nitric oxide (NO), a potent proinflammatory mediator, in these cells. RAW cells treated with CNT-attached PVDF showed reduced NO production in response to lipopolysaccharide. However, the same treatment also decreased the cell number suggesting that this treatment can alter the homeostasis of RAW cells. Although cell cycle of RAW cells was increased by PVDF treatment with or without CNTs, apoptosis was enhanced in these cells. Taken together, these results indicate that PVDF with or without CNTs modulates inflammatory responses possibly due to activation-induced cell death in macrophages.

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Single-Walled Carbon Nanotubes Induce Cell Death and Transcription of TNF-α in Macrophages Without Affecting Nitric Oxide Production

Cited by 9 publications

References 36 publications

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Specificity of carbon nanotube accumulation and distribution in cancer cells revealed by K-means clustering and principal component analysis of Raman spectra

Polyvinylidene Fluoride Alters Inflammatory Responses by Activation-induced Cell Death in Macrophages

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