Single‐walled carbon nanotubes modulate cardiovascular control in rats

Shapoval, L. M.; Prylutska, Svitlana; Kotsyuruba, A. V.; Дмитренко, Ольга Василівна; Prylutskyy, Yu. І.; Sagach, V. F.; Ritter, Uwe

doi:10.1002/mawe.201600484

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…It should be noted that SWCNT, GO and Dextran-PNIPAM NPs are non-toxic in vitro and in vivo in the concentration range under study (up to 6 mg mL -1 ). 26-28…”

Section: Resultsmentioning

confidence: 99%

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In vitro study of the anticancer activity of various doxorubicin-containing dispersions

et al. 2018

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Introduction: The aim of this research was to study the impact of various doxorubicin (Dox)-containing nanofluids, e.g. singlewalled carbon nanotube (SWCNT)+Dox, graphene oxide (GO)+Dox and DextranPNIPAM (copolymer)+Dox mixtures on HeLa cells (human transformed cervix epithelial cells, as a model for cancer cells) depending on their concentration. Methods: Structural analysis of GO+Dox complex was accomplished using Hartree-Fock level of theory in 6-31G** basis set in Gaussian. Dynamic light scattering (DLS), zeta-potential, scanning electron microscopy and confocal laser scanning microscopy were used. The cell viability was analyzed by the MTT assay. Results: The viability of HeLa cells was studied with the MTT assay after the incubation with various Dox-containing dispersions depending on their concentration. The size of the particles was determined by DLS. The morphology of the nanoparticles (NPs) was studied by scanning electron microscopy and their uptake into cells was visualized by confocal laser scanning microscopy. It was found that the Dextran-PNIPAM+Dox nanofluid in contrast to Dox alone showed higher toxicity towards HeLa cells up to 80% after 24 hours of incubation, whereas the SWCNT+Dox and GO+Dox nanofluids at the same concentrations protected cells from Dox. Conclusion: The importance of Dextran-PNIPAM copolymer as a universal platform for drug delivery was established, and the huge potential of Dextran-PNIPAM+Dox NPs as novel anticancer agents was noted. Based on the in vitro study of the SWCNT+Dox and GO+Dox nanofluids, it was concluded that SWCNT and GO NPs can be effective cytoprotectors against the highly toxic drugs.

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“…It should be noted that SWCNT, GO and Dextran-PNIPAM NPs are non-toxic in vitro and in vivo in the concentration range under study (up to 6 mg mL -1 ). 26-28…”

Section: Resultsmentioning

confidence: 99%

“…29 Thus, one can assume that coadministration of SWCNT and GO NPs with a high Dox dose is capable of ameliorating its cytotoxicity through their antioxidant potential. 15,26,28,30,31…”

Section: Resultsmentioning

confidence: 99%

In vitro study of the anticancer activity of various doxorubicin-containing dispersions

et al. 2018

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“…As a nanoplatform, SWCNTs can be used for imaging and drug delivery [11,12]. We were the first to show that SWCNTs modulate cardiovascular control in rats [13]. However, the biomedical applications of SWCNTs are limited by their possible toxicity, so their biosafety is currently one of the most discussed issues.…”

Section: Introductionmentioning

confidence: 99%

Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes

et al. 2021

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Single-walled carbon nanotubes (SWCNTs) are characterized by a combination of rather unique physical and chemical properties, which makes them interesting biocompatible nanostructured materials for various applications, including in the biomedical field. SWCNTs are not inert carriers of drug molecules, as they may interact with various biological macromolecules, including ion channels. To investigate the mechanisms of the inhibitory effects of SWCNTs on the muscarinic receptor cation current (mICAT), induced by intracellular GTPγs (200 μM), in isolated mouse ileal myocytes, we have used the patch-clamp method in the whole-cell configuration. Here, we use molecular docking/molecular dynamics simulations and direct patch-clamp recordings of whole-cell currents to show that SWCNTs, purified and functionalized by carboxylation in water suspension containing single SWCNTs with a diameter of 0.5–1.5 nm, can inhibit mICAT, which is mainly carried by TRPC4 cation channels in ileal smooth muscle cells, and is the main regulator of cholinergic excitation–contraction coupling in the small intestinal tract. This inhibition was voltage-independent and associated with a shortening of the mean open time of the channel. These results suggest that SWCNTs cause a direct blockage of the TRPC4 channel and may represent a novel class of TRPC4 modulators.

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Application of Nanoscale Materials and Nanotechnology Against Viral Infection: A Special Focus on Coronaviruses

Somu

Mohanty

Chakraborty

et al. 2021

Coronavirus Therapeutics – Volume I

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Single‐walled carbon nanotubes modulate cardiovascular control in rats

Cited by 8 publications

References 24 publications

In vitro study of the anticancer activity of various doxorubicin-containing dispersions

In vitro study of the anticancer activity of various doxorubicin-containing dispersions

Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes

Application of Nanoscale Materials and Nanotechnology Against Viral Infection: A Special Focus on Coronaviruses

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