Sinigrin (2-Propenyl Glucosinolate) Content and Myrosinase Activity inBrassicaVegetables

Bioactive Compounds, Myrosinase Activity, and Antioxidant Capacity of White Cabbages Grown in Different Locations of Spain

“…A large intraspecific variation for myrosinase activity among white cabbage cultivars has also been observed by Singh et al 33 although it was rather lower than those for the Spanish cabbages found in the present work.…”

Enhancing growth, phytochemical constituents and aphid resistance capacity in cabbage with foliar application of eckol – a biologically active phenolic molecule from brown seaweed

“…Myrosinase activity was assayed by the procedure described by Singh et al [16]. The extract used for the enzyme assay was RESEARCH PAPER New Biotechnology Volume 33, Number 2 March 2016 prepared in 20 mM potassium phosphate buffer, pH 7.4, containing 1 mM EDTA and 1 mM dithiothreitol (DTT), by grinding frozen tissues in a precooled mortar and pestle.…”

Evaluating the impact of sprouting conditions on the glucosinolate content of Brassica oleracea sprouts

“…Myrosinase is normally physically separated from the GLs in the cell, being localized in idioblasts (myrosin cells) . When plant cells are damaged (e.g., during food processing, ingestion and digestion, or injury by predators) the enzyme is released, promoting the hydrolysis of GLs, resulting in a range of breakdown biologically active compounds, like indoles and isothiocyanates (Kissen et al, 2009), and also glucose and sulphate (Singh et al, 2007). Different biological activities were attributed to these compounds, some beneficial, like the reduction of the risk of certain human's cancers (Fahey et al, 2001;Mithen et al, 2003), while others have a detrimental effect for humans and animals (Rosa et al, 1997).…”