Síntese e controle de qualidade da ureia enriquecida em 13C para diagnóstico da Helicobacterpylori (HP)

Filho, Carlos Roberto Sant Ana; Tavares, Claudinéia R. O.; Ferreira, André V.; Prestes, Clelber Vieira; Bendassolli, José Albertino

doi:10.1590/s0100-40422013000100020

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Further studies involving all ethical and regulatory aspects related to the development of a new drug should be performed to achieve registration with the Brazilian Health Regulatory Agency (Anvisa) before this test is entered into the market. The results described here should stimulate the development of large-scale production of 13 C-urea to facilitate the broader use of the UBT, and the subsequent technological production advances should stimulate a dramatic reduction in the cost of the substrate, which is currently estimated at approximately US $15.00 per test 12 .…”

Section: Discussionmentioning

confidence: 93%

“…One of the main barriers regarding the use of this test is the difficulty in obtaining the labeled isotope, as the technology for carbon separation (i.e., 13 C and 12 C) has not been transferred by the countries that developed it due to economic reasons and strategic interests. Recently, researchers from the Center of Nuclear Energy in Agriculture of the University of São Paulo (CENA/USP) were able to achieve the synthesis of urea ( 13 CO(NH 2 ) 2 ) with 99% 13 C atoms with a competitive production process and a reasonable cost compared with the international market value 12 .…”

Section: Introductionmentioning

confidence: 99%

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Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study

Coelho¹,

Sant'Ana²,

Oliveira³

et al. 2018

Clinics

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OBJECTIVE:The 13C-urea breath test is the main non-invasive test for the diagnosis of Helicobacter pylori infection. The availability of this test throughout the country is limited, mainly due to the difficulty in obtaining the labeled isotope from abroad. Recently, researchers from the Nuclear Energy Center in Agriculture at the University of São Paulo (CENA/USP) succeeded in synthesizing 13C-enriched urea for Helicobacter pylori diagnosis. The aim of the study was to compare the performance of the 13C-urea breath test using 13C-urea acquired abroad with that of a test using 13C-urea synthesized in Brazil.METHOD:Sixty-four dyspeptic patients participated in the study (24 men and 40 women). Initially, the patients performed the 13C-urea breath test using the imported substrate (Euriso-Top, France). Seven to fourteen days later, all the patients repeated the test using the Brazilian substrate. The samples from both examinations were processed in an infrared isotope analyzer (IRIS, Wagner Analisen Technik, Germany), and all delta over baseline (DOB) [%] values above four were considered positive results.RESULTS:Twenty-seven patients (42%) exhibited negative results for Helicobacter pylori infection, and thirty-seven patients (58%) exhibited positive results when tested using the foreign substrate (gold standard). There was a 100% concordance regarding the presence or absence of infection when the gold standard results were compared with those obtained using the Brazilian substrate.CONCLUSIONS:Similar performance in the diagnosis of Helicobacter pylori infection was demonstrated when using the 13C-urea breath test with the Brazilian 13C-urea substrate and the test with the substrate produced abroad. This validation represents an important step toward increasing the availability of the 13C-urea breath test throughout the country, which will have a positive influence on the management of Helicobacter pylori infection.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study

Coelho¹,

Sant'Ana²,

Oliveira³

et al. 2018

Clinics

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“…[3] Isotope-labelled urea, which is used for disease diagnoses and isotope tracing, is also produced through processes that consume organic matter and release harmful substances (Figure 1a). [4] Considering the global energy and environmental crisis, there is a need for the development of green chemical synthesis processes powered by renewable energy. The electricity-driven catalytic CÀ N coupling of CO 2 and nitrogenous small molecules, including nitrogen (N 2 ), [1a,5] nitric oxide (NO), [6] nitrite (NO 2 À ), [7] and nitrate (NO 3 À ), [8][9][10] has shown promise in achieving sustainable and direct urea synthesis under ambient conditions, by-passing the intermediate step of NH 3 synthesis.…”

Section: Introductionmentioning

confidence: 99%

A Universal Approach for Sustainable Urea Synthesis via Intermediate Assembly at the Electrode/Electrolyte Interface

Tu,

Zhu,

et al. 2023

Angew Chem Int Ed

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Electrocatalytic C−N coupling process is indeed a sustainable alternative for direct urea synthesis and co‐upgrading of carbon dioxide and nitrate wastes. However, the main challenge lies in the unactivated C−N coupling process. Here, we proposed a strategy of intermediate assembly with alkali metal cations to activate C−N coupling at the electrode/electrolyte interface. Urea synthesis activity follows the trend of Li+<Na+<Cs+<K+. In the presence of K+, a world‐record performance was achieved with a urea yield rate of 212.8±10.6 mmol h−1 g−1 on a single‐atom Co supported TiO2 catalyst at −0.80 V versus reversible hydrogen electrode. Theoretical calculations and operando synchrotron‐radiation Fourier transform infrared measurements revealed that the energy barriers of C−N coupling were significantly decreased via K+ mediated intermediate assembly. By applying this strategy to various catalysts, we demonstrate that intermediate assembly at the electrode/electrolyte interface is a universal approach to boost sustainable urea synthesis.

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A Universal Approach for Sustainable Urea Synthesis via Intermediate Assembly at the Electrode/Electrolyte Interface

Tu,

Zhu,

et al. 2023

Angewandte Chemie

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Síntese e controle de qualidade da ureia enriquecida em 13C para diagnóstico da Helicobacterpylori (HP)

Cited by 3 publications

References 19 publications

Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study

Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study

A Universal Approach for Sustainable Urea Synthesis via Intermediate Assembly at the Electrode/Electrolyte Interface

A Universal Approach for Sustainable Urea Synthesis via Intermediate Assembly at the Electrode/Electrolyte Interface

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