Sirtuin 7 Regulates Nitric Oxide Production and Apoptosis to Promote Mycobacterial Clearance in Macrophages

Zhang, Su; Liu, Yaya; Zhou, Xuefeng; Ou, Min; Xiao, Guohui; Li, Fang; Wang, Zhongyuan; Wang, Zhaoqin; Liu, Lei; Zhang, Guoliang

doi:10.3389/fimmu.2021.779235

Cited by 17 publications

(13 citation statements)

References 35 publications

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“…In fact, the signaling pathways and biological processes associated with innate immune responses are highly conserved in Drosophila and vertebrates. For instance, nuclear factor-kappa B (NF-kB) signaling, phagocytosis and apoptosis are evident in the innate immunity of both vertebrates and invertebrates (4)(5)(6)(7)(8). Hence, it is possible to utilize insect models such as flies and nematodes to investigate pathogen and host interactions.…”

Section: Introductionmentioning

confidence: 99%

Drosophila Innate Immunity Involves Multiple Signaling Pathways and Coordinated Communication Between Different Tissues

Luo

et al. 2022

Front. Immunol.

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The innate immune response provides the first line of defense against invading pathogens, and immune disorders cause a variety of diseases. The fruit fly Drosophila melanogaster employs multiple innate immune reactions to resist infection. First, epithelial tissues function as physical barriers to prevent pathogen invasion. In addition, macrophage-like plasmatocytes eliminate intruders through phagocytosis, and lamellocytes encapsulate large particles, such as wasp eggs, that cannot be phagocytosed. Regarding humoral immune responses, the fat body, equivalent to the mammalian liver, secretes antimicrobial peptides into hemolymph, killing bacteria and fungi. Drosophila has been shown to be a powerful in vivo model for studying the mechanism of innate immunity and host-pathogen interactions because Drosophila and higher organisms share conserved signaling pathways and factors. Moreover, the ease with which Drosophila genetic and physiological characteristics can be manipulated prevents interference by adaptive immunity. In this review, we discuss the signaling pathways activated in Drosophila innate immunity, namely, the Toll, Imd, JNK, JAK/STAT pathways, and other factors, as well as relevant regulatory networks. We also review the mechanisms by which different tissues, including hemocytes, the fat body, the lymph gland, muscles, the gut and the brain coordinate innate immune responses. Furthermore, the latest studies in this field are outlined in this review. In summary, understanding the mechanism underlying innate immunity orchestration in Drosophila will help us better study human innate immunity-related diseases.

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Section: Introductionmentioning

confidence: 99%

Drosophila Innate Immunity Involves Multiple Signaling Pathways and Coordinated Communication Between Different Tissues

Luo

et al. 2022

Front. Immunol.

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“…Therefore, we examined all sirtuin factors ( Supplementary Figure S5 ) and found that only Sirt7, reduced by prior LPS stimulation, was significantly increased in abundance under hesperetin supplementation. Previous studies have demonstrated that Sirt7 can regulate inflammation, OS, and apoptosis, and its beneficial effects have been reported in the vascular endothelium and many other cardiovascular models [ 37 , 38 , 39 ]. However, the role of Sirt7 in CAVD is incompletely understood, and the relationship between Sirt7 and Nrf2 remains undefined.…”

Section: Resultsmentioning

confidence: 99%

Hesperetin, a Promising Dietary Supplement for Preventing the Development of Calcific Aortic Valve Disease

et al. 2022

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Background: No effective therapeutic agents for calcific aortic valve disease (CAVD) are available currently. Dietary therapy has been proposed as a novel treatment strategy for various diseases. As a flavanone, hesperetin is widely abundant in citrus fruits and has been proven to play a protective role against multiple diseases. However, the role of hesperetin in CAVD remains unclear. Methods: Human aortic valve interstitial cells (VICs) were isolated from aortic valve leaflets. A mouse model of aortic valve stenosis was constructed through direct wire injury (DWI). Immunoblotting, immunofluorescence staining, and flow cytometry were used to investigate the roles of sirtuin 7 (Sirt7) and nuclear factor erythroid 2-related factor 2 (Nrf2) in hesperetin-mediated protective effects in VICs. Results: Hesperetin supplementation protected the mice from wire-injury-induced aortic valve stenosis; in vitro, hesperetin suppressed the lipopolysaccharide (LPS)-induced activation of NF-κB inflammatory cytokine secretion and osteogenic factors expression, reduced ROS production and apoptosis, and abrogated LPS-mediated injury to the mitochondrial membrane potential and the decline in the antioxidant levels in VICs. These benefits of hesperetin may have been obtained by activating Nrf2–ARE signaling, which corrected the dysfunctional mitochondria. Furthermore, we found that hesperetin could directly bind to Sirt7 and that the silencing of Sirt7 decreased the effects of hesperetin in VICs and potently abolished the ability of hesperetin to increase Nrf2 transcriptional activation. Conclusions: Our work demonstrates that hesperetin plays protective roles in the aortic valve through the Sirt7–Nrf2–ARE axis; thus, hesperetin might be a potential dietary supplement that could prevent the development of CAVD.

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“…Another study described the critical role of caspase-8 in driving the cell death of M.tb -infected mouse macrophages and the control of infection in mice by enhancing apoptosis through the use of specific treatments [ 9 ]. Sirtuin 7 was another factor described to help control M.tb growth through NO-induced apoptosis in RAW 264.7 cells [ 10 ]. There has also been an increasing interest in studying the function of different microRNAs in the context of programmed cell death [ 76 , 77 , 78 , 79 , 80 ].…”

Section: Cellular Pathwaysmentioning

confidence: 99%

Resistance and Susceptibility Immune Factors at Play during Mycobacterium tuberculosis Infection of Macrophages

et al. 2022

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Tuberculosis (TB), caused by infection with Mycobacterium tuberculosis (M.tb), is responsible for >1.5 million deaths worldwide annually. Innate immune cells, especially macrophages, are the first to encounter M.tb, and their response dictates the course of infection. During infection, macrophages exert a variety of immune factors involved in either controlling or promoting the growth of M.tb. Research on this topic has been performed in both in vitro and in vivo animal models with discrepant results in some cases based on the model of study. Herein, we review macrophage resistance and susceptibility immune factors, focusing primarily on recent advances in the field. We include macrophage cellular pathways, bioeffector proteins and molecules, cytokines and chemokines, associated microbiological factors and bacterial strains, and host genetic factors in innate immune genes. Recent advances in mechanisms underlying macrophage resistance and susceptibility factors will aid in the successful development of host-directed therapeutics, a topic emphasized throughout this review.

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Sirtuin 7 Regulates Nitric Oxide Production and Apoptosis to Promote Mycobacterial Clearance in Macrophages

Cited by 17 publications

References 35 publications

Drosophila Innate Immunity Involves Multiple Signaling Pathways and Coordinated Communication Between Different Tissues

Drosophila Innate Immunity Involves Multiple Signaling Pathways and Coordinated Communication Between Different Tissues

Hesperetin, a Promising Dietary Supplement for Preventing the Development of Calcific Aortic Valve Disease

Resistance and Susceptibility Immune Factors at Play during Mycobacterium tuberculosis Infection of Macrophages

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