Sister chromatid exchanges and chromosomal aberrations in Chinese hamster ovary (CHO-K1) cells treated with the insecticide pirimicarb

Soloneski, Sonia; Larramendy, Marcelo L.

doi:10.1016/j.jhazmat.2009.09.068

Cited by 46 publications

(62 citation statements)

References 38 publications

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“…Similar positive results were found when the sister chromatid exchange assay was performed in Chinese hamster ovary cells (Soloneski & Larramendy, 2010). Although a significant increase in chromosomal aberrations has been reported in Chinese hamster ovary cells after pirimicarb exposure (Soloneski & Larramendy, 2010), Wildgoose and coworkers (1987) observed negative results in human lymphocytes with or without S9 metabolic activation. Finally, the induction of alterations in the cell-cycle progression in Chinese hamster ovary cells was reported to occur after in vitro exposure to pirimicarb (Soloneski & Larramendy, 2010).…”

Section: Pirimicarb Genotoxicity and Cytotoxicity Profilessupporting

confidence: 59%

“…Furthermore, the induction of DNA single strand breaks, estimated by the alkaline comet assay, was evaluated revealing positive results in human lymphocytes exposed in vitro to pirimicarb (Ündeger & Basaran, 2005). Similar positive results were found when the sister chromatid exchange assay was performed in Chinese hamster ovary cells (Soloneski & Larramendy, 2010). Although a significant increase in chromosomal aberrations has been reported in Chinese hamster ovary cells after pirimicarb exposure (Soloneski & Larramendy, 2010), Wildgoose and coworkers (1987) observed negative results in human lymphocytes with or without S9 metabolic activation.…”

Section: Pirimicarb Genotoxicity and Cytotoxicity Profilesmentioning

confidence: 54%

“…Although a significant increase in chromosomal aberrations has been reported in Chinese hamster ovary cells after pirimicarb exposure (Soloneski & Larramendy, 2010), Wildgoose and coworkers (1987) observed negative results in human lymphocytes with or without S9 metabolic activation. Finally, the induction of alterations in the cell-cycle progression in Chinese hamster ovary cells was reported to occur after in vitro exposure to pirimicarb (Soloneski & Larramendy, 2010). In in vivo genotoxic and cytotoxic studies, pirimicarb was able to induce different types of lesions.…”

Section: Pirimicarb Genotoxicity and Cytotoxicity Profilesmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Toxicological Profile of Carbofuran and Pirimicarb Carbamic Insecticides

Soloneski¹,

Larramendy²

2012

Insecticides - Pest Engineering

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Section: Pirimicarb Genotoxicity and Cytotoxicity Profilessupporting

confidence: 59%

Section: Pirimicarb Genotoxicity and Cytotoxicity Profilesmentioning

confidence: 54%

Section: Pirimicarb Genotoxicity and Cytotoxicity Profilesmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Toxicological Profile of Carbofuran and Pirimicarb Carbamic Insecticides

Soloneski¹,

Larramendy²

2012

Insecticides - Pest Engineering

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“…Genotoxicity and cytotoxicity studies are frequently used to test numerous agrochemical compounds with different test systems (Ergene et al 2007;Lin and Garry 2000;Rakitsky et al 2000;Soloneski et al 2007Soloneski et al , 2008Soloneski and Larramendy 2010;Zeljezic et al 2006). These test systems are valuable and very well-known tools for the early and sensitive detection or estimation of genotoxic potential of chemicals.…”

Section: Introductionmentioning

confidence: 99%

Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

et al. 2014

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This study evaluates the cytotoxic and genotoxic potential of alloxydim sodium using micronucleus (MN) assay, in human peripheral lymphocytes. MN assay was used to investigate the genotoxic effects of alloxydim sodium in human peripheral lymphocytes treated with 250, 500, 750, 1,000 lg/ml concentrations of alloxydim sodium for 24 and 48 h. Solvent, negative and positive controls were also used in the experiments in parallel. The obtained results were evaluated in statistical analyses by using Dunnett-t test (two sided) and p \ 0.05 was accepted as significant. Alloxydim sodium significantly increased the MN formation compared with the negative control, at both 750 and 1,000 lg/ml concentrations and treatment periods. We also evaluated the nuclear division index (NDI) for cytotoxicity of this pesticide in the experiment, and finally observed a significant decrease of the NDI values at all concentrations of alloxydim sodium and at both treatment periods.

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“…Despite the beneficial effects associated with the use of agrochemicals in agriculture and households, many of these products could be potentially hazardous because of their extensive use (1). Among various types of agrochemicals, large quantities of carbamates are particularly applied to different crops.…”

mentioning

confidence: 99%

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

Valencia-Quintana

Gómez‐Arroyo

Sánchez-Alarcón

et al. 2016

Archives of Industrial Hygiene and Toxicology

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This study evaluated direct and metabolic genotoxic effects caused by Lannate-90 ® , a methomyl-based formulation (90 % active ingredient), in human lymphocyte cultures using sister chromatid exchange assay (SCE). Two processes were used for the plant promutagens evaluation: in vivo activation, applying the insecticide systemically in plants for 4 h and subsequently adding plant metabolites containing extracts to lymphocyte cultures; and in vitro activation, where the insecticide was incubated with Vicia faba S10 mix plus human lymphocyte culture. Direct treatment with the insecticide significantly increased SCE frequency in human lymphocytes (250-750 mg L ® to treat human lymphocytes, a dose-response relationship was observed. In lymphocyte cultures treated directly with the insecticide for 2 h, a negative response was obtained. When S10 mix was added, SCE frequency did not change significantly. Meanwhile, a mixture of S9 mammalian metabolic mix and Lannate-90 ® increased the SCE frequency, with an observed concentration-dependent response. Although Lannate-90 ® induced cellular death at the highest concentrations, it did not cause a delay in cell proliferation in any of the treatments, confirming its genotoxic action. This study is one of the first to evaluate and compare the direct effect of Lannate-90 ® in two bioassays, animal and vegetal, and the effect of plant and animal metabolism on its genotoxic potential.

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Sister chromatid exchanges and chromosomal aberrations in Chinese hamster ovary (CHO-K1) cells treated with the insecticide pirimicarb

Cited by 46 publications

References 38 publications

Genetic Toxicological Profile of Carbofuran and Pirimicarb Carbamic Insecticides

Genetic Toxicological Profile of Carbofuran and Pirimicarb Carbamic Insecticides

Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

Assessment of genotoxicity of Lannate-90® and its plant and animal metabolites in human lymphocyte cultures

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