Site of cholesterol absorption in some insects

Joshi, Madhu; Agarwal, H. C.

doi:10.1016/0022-1910(77)90280-3

Cited by 28 publications

(6 citation statements)

References 5 publications

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“…These data demonstrated that the midgut is the major site of cholesterol absorption and release into the hemolymph. Other lepidopterans have also been shown to absorb sterols in the midgut (Joshi and Agarwal, 1977).…”

Section: Localization Of Cholesterol Absorption In the Gutmentioning

confidence: 99%

Absorption and tissue distribution of cholesterol in Manduca sexta

Jouni

Zamora

Wells

2002

Arch Insect Biochem Physiol

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In Manduca sexta larvae, radioactive free cholesterol is absorbed directly from the midgut into mucosal cells where it is stored both in the free form (87% in males and 93% in females) and esterified form (13% in males and 7% in females). Subsequently, cholesterol is transported to fat body via lipophorin in the hemolymph exclusively in the free form. In fat body, the distribution of cholesterol between the free and esterified form varied significantly between genders and developmental stages. Except for the larval stage, males and females were able to store cholesterol in both free and esterified forms in the fat body and in the adult stage cholesterol ester accounted for more than 75% of the stored cholesterol. Placement of radioactive cholesterol at different locations in the gut-foregut, midgut, and hindgut-demonstrated that the midgut is the site where cholesterol is absorbed and released into the hemolymph. Cholesterol-labeled lipophorin injected into larval hemolymph was cleared from the hemolymph with a half-life of 10.2 h. After 17 h, most of the cleared radioactivity was recovered in the fat body (38%). Arch.

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Section: Localization Of Cholesterol Absorption In the Gutmentioning

confidence: 99%

Absorption and tissue distribution of cholesterol in Manduca sexta

Jouni

Zamora

Wells

2002

Arch Insect Biochem Physiol

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“…As phytosterols play an important role in sterol metabolism and ecdysteroid production [8,10,[23][24][25][26], the ingestion of exogenous sterols is essential for structural and metabolic components. Sterols seem to be absorbed mainly in the midgut, particularly in phytophagous species [27,28], moving quickly through mucosal cells [28]. The analyzed pollen and glands lacked 24-methylenecholesterol, differently from results found for Apis mellifera by Svoboda et al [1].…”

Section: Discussionmentioning

confidence: 69%

Neutral Sterols of Cephalic Glands of Stingless Bees and Their Correlation with Sterols from Pollen

Ferreira-Caliman

Silva

Mateus

et al. 2012

Psyche: A Journal of Entomology

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Sterols are essential molecules in the membrane lipid composition and precursors of important sterol hormones that regulate many developmental processes. Insects are unable to synthesize sterols de novo and, thus, all phytophagous insects depend on an exogenous source of sterols for growth, development, and reproduction. The sterol requirements of social bees are not fully known due to the fact that there is no well-defined diet available throughout the year with regard to floral resources. Our study aimed to characterize the sterols present in pollen stored in Melipona marginata and Melipona scutellaris colonies, as well as evaluating their presence in the mandibular, hypopharyngeal, and cephalic salivary gland secretions. We analyzed the chemical composition of pollen stored in the colonies and the composition of the cephalic glands of workers in three adult functional phases (newly emerged, nurses, and foragers) by gas chromatography and mass spectrometry. The results showed that the pollen analyzed contained campesterol, stigmasterol, sitosterol, isofucosterol, lanosterol, and small amounts of cholesterol. The glands showed the same compounds found in the pollen analyzed, except lanosterol that was not found in M. scutellaris glands. Surprisingly, cholesterol was found in some glands with relative ratios greater than those found in pollen.

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“…The water should be brought to boil and the mixture strained through grass to remove particles such as parts of bees, before being boiled again (Kritsky 2015). It is important to stress that bees use sterols in a variety of key metabolic pathways, as the production of their moulting hormones (Joshi and Agarwal 1977;Svoboda et al 1986;Vanderplanck et al 2011). Therefore, in the absence of any other explanation, one plausible hypothesis to justify the existence of cholesterol in ancient honey is the presence of parts of bees that would contaminate the honey (Ferreira-Caliman et al 2012;Ikekawa et al 1993;Joshi and Agarwal 1977).…”

Section: Main Results and Discussionmentioning

confidence: 99%

Chromatographic analysis of honey ceramic artefacts

Oliveira

Araújo

Ribeiro

et al. 2017

Archaeol Anthropol Sci

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Honey was the main sweetening agent in the ancient world; it is mentioned in literary sources and archaeologically documented all over the Mediterranean basin. Its production extended throughout different parts of the Roman Empire, with important economic impacts on family economies, both as a foodstuff and as a commercial product. This paper focuses on the chromatographic analysis of Roman Iberian honeypots and ceramic beehives, identifying tracer characteristics from ancient honey in sherds from seven honeypots and three beehives. The obtained results present an important contribution to the knowledge of the local Roman Iberian economy, particularly relating to the production, transport and trade of honey in locally manufactured ceramic containers.

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Site of cholesterol absorption in some insects

Cited by 28 publications

References 5 publications

Absorption and tissue distribution of cholesterol in Manduca sexta

Absorption and tissue distribution of cholesterol in Manduca sexta

Neutral Sterols of Cephalic Glands of Stingless Bees and Their Correlation with Sterols from Pollen

Chromatographic analysis of honey ceramic artefacts

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