Site Specific Analysis of Anionic Lipid by Membrane Surface-enhanced Raman Spectroscopy with Different Sized Gold Nanoparticles

Faried, Miftah; Ando, Seiya; Suga, Kazuyoshi; Okamoto, Yukihiro; Umakoshi, Hiroshi

doi:10.1246/cl.200389

Cited by 1 publication

(3 citation statements)

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“…To bridge this inconsistency, methods that probe their structures in environments that mimic a biological system are required. (Reproduced from [77] with permission).…”

Section: Discussionmentioning

confidence: 99%

“…The incubation of liposomes with metal nanoparticles [77][78][79][80] is simple and does not require much preparation, with quality of data largely being dependent on the quality of the nanoparticles. The method has proved to be useful for lipid membrane analysis [81].…”

Section: Ramanmentioning

confidence: 99%

“…Examples of how SERS can be used with liposomes are provided by Faried et al [77] who performed location-specific analysis on the DMPG membranes (Figure 10) using 1-octanethiol capped gold nanoparticles of sizes 100 nm and 5 nm. The larger 100 nm particles preferentially enhanced signals from the surface region of the monolayer (e.g.…”

Section: Ramanmentioning

confidence: 99%

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Spectroscopy of model-membrane liposome-protein systems: complementarity of linear dichroism, circular dichroism, fluorescence and SERS

Rodger

2021

Emerging Topics in Life Sciences

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A range of membrane models have been developed to study components of cellular systems. Lipid vesicles or liposomes are one such artificial membrane model which mimics many properties of the biological system: they are lipid bilayers composed of one or more lipids to which other molecules can associate. Liposomes are thus ideal to study the roles of cellular lipids and their interactions with other membrane components to understand a wide range of cellular processes including membrane disruption, membrane transport and catalytic activity. Although liposomes are much simpler than cellular membranes, they are still challenging to study and a variety of complementary techniques are needed. In this review article, we consider several currently used analytical methods for spectroscopic measurements of unilamellar liposomes and their interaction with proteins and peptides. Among the variety of spectroscopic techniques seeing increasing application, we have chosen to discuss: fluorescence based techniques such as FRET (fluorescence resonance energy transfer) and FRAP (fluorescence recovery after photobleaching), that are used to identify localisation and dynamics of molecules in the membrane; circular dichroism (CD) and linear dichroism (LD) for conformational and orientation changes of proteins on membrane binding; and SERS (Surface Enhanced Raman Spectroscopy) as a rapidly developing ultrasensitive technique for site-selective molecular characterisation. The review contains brief theoretical basics of the listed techniques and recent examples of their successful applications for membrane studies.

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“…To bridge this inconsistency, methods that probe their structures in environments that mimic a biological system are required. (Reproduced from [77] with permission).…”

Section: Discussionmentioning

confidence: 99%