Site specific biotinylated antibody functionalized Ag@AuNIs LSPR biosensor for the ultrasensitive detection of exosomal MCT4, a glioblastoma progression biomarker

Liu, Linlin; Thakur, Abhimanyu; Li, Wing Kar; Qiu, Guangyu; Yang, Tian; He, Bing; Lee, Youngjin; Wu, Chi‐Man Lawrence

doi:10.1016/j.cej.2022.137383

Cited by 32 publications

(24 citation statements)

References 53 publications

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“…Liu et al developed a localized surface plasmon resonance (LSPR) sensor chip that could detect small amounts of exosomal biomarkers. 54 Self-assembly silver nanoparticles decorated on gold nano-islands (Ag@AuNIs) sensor chip were used to provide site-specific bio-conjunction of biotinylated antibodies (anti-CD9 ABs, anti-CD63 ABs, anti-EGFRvIII (epidermal growth factor receptor variant III) ABs, anti-MCT4 ABs) for the detection of exosomal surface biomarkers. GM-derived exosomes were isolated from the blood serum of glioblastoma multiform (GBM) mice using an EGFRvIII-based immunocapture method.…”

Section: Electrochemicalmentioning

confidence: 99%

Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations

Shaabani

Meira

Marcet‐Palacios

et al. 2023

ACS Pharmacol. Transl. Sci.

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Extracellular vesicles (EVs) are an important intercellular communication conduit for cells that have applications in precision therapy and targeted drug delivery. Small EVs, or exosomes, are a 30−150 nm phospholipid-encased subpopulation of EVs that are particularly difficult to characterize due to their small size and because they are difficult to isolate using conventional methods. In this review, we discuss some recent advances in exosome isolation, purification, and sensing platforms using microfluidics, acoustics, and size exclusion chromatography. We discuss some of the challenges and unanswered questions with respect to understanding exosome size heterogeneity and how modern biosensor technology can be applied to exosome isolation. In addition, we discuss how some advancements in sensing platforms such as colorimetric, fluorescent, electronic, surface plasmon resonance (SPR), and Raman spectroscopy may be applied to exosome detection in multiparametric systems. The application of cryogenic electron tomography and microscopy to understanding exosome ultrastructure will become vital as this field progresses. In conclusion, we speculate on some future needs in the exosome research field and how these technologies could be applied.

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Section: Electrochemicalmentioning

confidence: 99%

Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations

Shaabani

Meira

Marcet‐Palacios

et al. 2023

ACS Pharmacol. Transl. Sci.

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“…Recently, an LSPR biosensor chip was developed based on the immobilization of antibodies on the surface of Au nano-islands decorated with AgNPs (Ag@AuNIs) for the detection of monocarboxylate transporter 4 (MCT4) as a GBM progression biomarker [ 93 ]. The anti-MCT4 antibodies on the surface of Ag@AuNIs-modified chips had a remarkable ability to detect exosomes, resulting in the generation of a strong LSPR response.…”

Section: Developed Biosensors For Childhood Cancers In Clinical Practicementioning

confidence: 99%

Biomarkers and Corresponding Biosensors for Childhood Cancer Diagnostics

Gharehzadehshirazi

Zarejousheghani

Falahi

et al. 2023

Sensors

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Although tremendous progress has been made in treating childhood cancer, it is still one of the leading causes of death in children worldwide. Because cancer symptoms overlap with those of other diseases, it is difficult to predict a tumor early enough, which causes cancers in children to be more aggressive and progress more rapidly than in adults. Therefore, early and accurate detection methods are urgently needed to effectively treat children with cancer therapy. Identification and detection of cancer biomarkers serve as non-invasive tools for early cancer screening, prevention, and treatment. Biosensors have emerged as a potential technology for rapid, sensitive, and cost-effective biomarker detection and monitoring. In this review, we provide an overview of important biomarkers for several common childhood cancers. Accordingly, we have enumerated the developed biosensors for early detection of pediatric cancer or related biomarkers. This review offers a restructured platform for ongoing research in pediatric cancer diagnostics that can contribute to the development of rapid biosensing techniques for early-stage diagnosis, monitoring, and treatment of children with cancer and reduce the mortality rate.

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“…Wu et al fabricated a gold nanoisland (AuNIs)-based localized surface plasmon resonance (LSPR) biosensor decorated with silver nanoparticles to detect glioblastoma-derived exosomes [ 97 ]. The proposed sensor ( Figure 2 A), composed of a biotinylated antibody targeting MCT4 on the exosome surface, has excellent corrosion resistance, ease of nanostructure fabrication, and the ability to generate plasmon resonance through light in the mid-range of visible light and in the visible light of Ag.…”

Section: Nanobiomaterial-based Exosome Biosensormentioning

confidence: 99%

“… ( A ) Schematic diagram of gold nanoisland−based LSPR biosensor, ( B ) performance evaluation of gold nanoisland-based sensor according to exosome concentration versus LSPR response, ( C ) detection strategy of gold nanoparticle DNA motor-based fluorescent sensor, and ( D ) performance evaluation of fluorescent sensor according to wavelength and exosome concentration. This was reproduced with permission from [ 49 , 97 ], published by Elsevier, 2022 and 2020, respectively. …”

Section: Figurementioning

confidence: 99%

Introduction of Nanomaterials to Biosensors for Exosome Detection: Case Study for Cancer Analysis

et al. 2022

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Exosomes have been gaining attention for early cancer diagnosis owing to their biological functions in cells. Several studies have reported the relevance of exosomes in various diseases, including pancreatic cancer, retroperitoneal fibrosis, obesity, neurodegenerative diseases, and atherosclerosis. Particularly, exosomes are regarded as biomarkers for cancer diagnosis and can be detected in biofluids, such as saliva, urine, peritoneal fluid, and blood. Thus, exosomes are advantageous for cancer liquid biopsies as they overcome the current limitations of cancer tissue biopsies. Several studies have reported methods for exosome isolation, and analysis for cancer diagnosis. However, further clinical trials are still required to determine accurate exosome concentration quantification methods. Recently, various biosensors have been developed to detect exosomal biomarkers, including tumor-derived exosomes, nucleic acids, and proteins. Among these, the exact quantification of tumor-derived exosomes is a serious obstacle to the clinical use of liquid biopsies. Precise detection of exosome concentration is difficult because it requires clinical sample pretreatment. To solve this problem, the use of the nanobiohybrid material-based biosensor provides improved sensitivity and selectivity. The present review will discuss recent progress in exosome biosensors consisting of nanomaterials and biomaterial hybrids for electrochemical, electrical, and optical-based biosensors.

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Site specific biotinylated antibody functionalized Ag@AuNIs LSPR biosensor for the ultrasensitive detection of exosomal MCT4, a glioblastoma progression biomarker

Cited by 32 publications

References 53 publications

Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations

Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations

Biomarkers and Corresponding Biosensors for Childhood Cancer Diagnostics

Introduction of Nanomaterials to Biosensors for Exosome Detection: Case Study for Cancer Analysis

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