Six unusual dextrans: methylation structural analysis by combined g.l.c.—m.s. of per-O-acetyl-aldononitriles

Seymour, Fred R.; Slodki, Mores E.; Plattner, Ronald D.; Jeanes, Allene

doi:10.1016/s0008-6215(00)88083-0

Cited by 94 publications

(19 citation statements)

References 51 publications

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“…This combination of residues was the same as that found in the B1355S and B1501S glucans isolated from Leuconostoc citreum strains NRRL B-1355 and NRRL B-1501 (Jeanes et al, 1954;Seymour et al, 1979b;Seymour, Slodki, Plattner, & Jeanes., 1977). (The same strains produce in addition the polysaccharides B1355L and B1501L which are dextrans containing over 90% (1,6)-disubstituted glucose residues (Seymour, Chen, & Bishop, 1979)).…”

Section: Methylation Analysissupporting

confidence: 75%

Structural analysis of the α-d-glucan produced by the sourdough isolate Lactobacillus brevis E25

et al. 2018

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Section: Methylation Analysissupporting

confidence: 75%

Structural analysis of the α-d-glucan produced by the sourdough isolate Lactobacillus brevis E25

et al. 2018

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“…Since the 1950s, L. citreum NRRL B-1299 has received particular attention due to its ability to synthesize an unusual dextran containing up to 35% a-(1?2) linkages [26,[46][47][48][49]. Here we provide the assembled genome for this strain.…”

Section: Discussionmentioning

confidence: 99%

Inventory of the GH70 enzymes encoded by Leuconostoc citreum NRRL B‐1299 – identification of three novel α‐transglucosylases

et al. 2015

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Leuconostoc citreum NRRL B‐1299 has long been known to produce α‐glucans containing both α‐(1→6) and α‐(1→2) linkages, which are synthesized by α‐transglucosylases of the GH70 family. We sequenced the genome of Leuconostoc citreum NRRL B‐1299 to identify the full inventory of GH70 enzymes in this strain. Three new genes (brsA, dsrM and dsrDP) putatively encoding GH70 enzymes were identified. The corresponding recombinant enzymes were characterized. Branching sucrase A (BRS‐A) grafts linear α‐(1→6) dextran with α‐(1→2)‐linked glucosyl units, and is probably involved in the α‐(1→2) branching of L. citreum NRRL B‐1299 dextran. This is the first report of a naturally occurring α‐(1→2) branching sucrase. DSR‐M and DSR‐DP are dextransucrases that are specific for α‐(1→6) linkage synthesis and mainly produce oligomers or short dextrans with molar masses between 580 and 27 000 g·mol−1. In addition, DSR‐DP contains sequences that diverge from the consensus sequences that are typically present in enzymes that synthesize linear dextran. Comparison of the genome with five other L. citreum genomes further revealed that dsrDP is unique to L. citreum NRRL B‐1299. The presence of this gene in a prophage represents the first evidence of phage‐mediated horizontal transfer of genes encoding such enzymes in lactic acid bacteria. Finally, brsA and dsrM are located in a chromosomal region in which genes encoding strain‐specific GH70 enzymes are consistently located. This region may be a good target on which to focus in order to rapidly evaluate the diversity of GH70 enzymes in L. citreum strains.

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“…Proton use of Bruker ARX spectrometer equipped with a 5-mm 'W I3C dual probe, at 400.13 MHz ('Hj and 100.61MHz ("Cj. Methylation analyses were carried out according to published procedures [24]. Chemical ionization mass spectrometry was carried out in a Finnegan TSQ 700 instrument, with ammonia as the reagent gas.…”

Section: Methodsmentioning

confidence: 99%

Enzymically Produced Cyclic α‐1,3‐Linked and α‐1,6‐Linked Oligosaccharides of d‐Glucose

Côté

Biely

1994

European Journal of Biochemistry

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A new type of bacterial enzyme hydrolyzed alternan (Leuconostoc mesenteroides NRRL B-1355 fraction S dextran, an alternating a-l,3-a-1,6-D-glucan) to give rise to a series of oligosaccharides. The oligosaccharide formed in the greatest proportion was a cyclic tetrasaccharide of D-glucosyl residues linked in an alternating a-1,3-a-1,6 fashion. Other saccharide products included isomaltose and a-D-glucopyranosyl-1,3-a-D-g~UCOp~~~~OS~~-~,6-~-glucose. Oligosaccharides of higher degrees of polymerization were also formed, and included a-D-glucosyhted derivatives of the cyclic tetrasaccharide. This is the first report of a naturally produced cyclic tetrasaccharide.Relatively few examples are known of cyclic oligosaccharides, the best known being the cyclodextrins [l]. Other cyclic oligosaccharides include the p-1,3-p-1,6-linked glucooligosaccharides of Bradyrhizobiurn spp. Alternan, also referred to in the literature as B-1355 fraction S dextran [16], is produced from sucrose by Leuconostoc rnesenteroides NRRL B-1355 [17]. It is composed predominantly of an alternating sequence of a-1,3-linked and a-1,6-linked D-glucose residues, with approximately 10% branching [18]. While studying the specificity of a new class of enzymes that degrade alternan, we noted that some of the Correspondence to G.

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Six unusual dextrans: methylation structural analysis by combined g.l.c.—m.s. of per-O-acetyl-aldononitriles

Cited by 94 publications

References 51 publications

Structural analysis of the α-d-glucan produced by the sourdough isolate Lactobacillus brevis E25

Structural analysis of the α-d-glucan produced by the sourdough isolate Lactobacillus brevis E25

Inventory of the GH70 enzymes encoded by Leuconostoc citreum NRRL B‐1299 – identification of three novel α‐transglucosylases

Enzymically Produced Cyclic α‐1,3‐Linked and α‐1,6‐Linked Oligosaccharides of d‐Glucose

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