Size-adjustable self-assembled nanoparticles through microfluidic platform promotes neuronal differentiation of mouse embryonic stem cells

Karimi‐Soflou, Reza; Karkhaneh, Akbar; Shabani, Iman

doi:10.1016/j.bioadv.2022.213056

Cited by 9 publications

(7 citation statements)

References 59 publications

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“…are shown in Tables 5 and 6 . 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168…”

Section: Application Of Microfluidic Technology In Ddssunclassified

“…In contrast to traditional methods of micelle fabrication, microfluidics demonstrates a significant advantage in the production of micelles. In this process, polymeric precursors dissolved in a water-miscible organic solvent are introduced into a microchannel (HFF mixer 158 or Tesla mixer 161 ) and mixed with water. This interaction induces the aggregation of the hydrophobic segments of the amphiphilic copolymers, leading to the self-assembly of micelles.…”

Section: Micellesmentioning

confidence: 99%

“…This interaction induces the aggregation of the hydrophobic segments of the amphiphilic copolymers, leading to the self-assembly of micelles. 158 Karimi-Soflou et al utilized the HFF mixer to create micelles designed for intracellular retinoic acid release on demand. These micelles offer adjustable sizes and precisely controlled physio-biological properties, thereby facilitating efficient neuronal differentiation.…”

Section: Micellesmentioning

confidence: 99%

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Research Strategies for Precise Manipulation of Micro/Nanoparticle Drug Delivery Systems Using Microfluidic Technology: A Review

Liu,

Fu,

et al. 2024

Pharmaceutical Fronts

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Microfluidic technology facilitates precise control over fluid mixing and interactions between the components, including self-assembly and precipitation. It offers new options for accurately manufacturing particles and holds significant potential in advancing micro/nanoparticle drug delivery systems (DDSs). Various microchannel/microfluidic chips have been explored to construct micro/nanoparticle DDSs. The precise manipulation of particle size, morphology, structure, stiffness, surface characteristics, and elasticity through microfluidic technology relies on specific microchannel geometrical designs and the application of exogenous energy, adhering to the principles of fluid motion. Consequently, this enables reproducible control over critical quality attributes (CQAs), such as particle size and distribution, encapsulation efficiency, drug loading, in vitro and in vivo drug delivery profiles, Zeta potential, and targeting capabilities, for micro/nanoparticle DDSs. In this review, we categorize microfluidic techniques and explore recent research developments in novel microchannel structures spanning the past 5 years (2018–2023) and their applications in micro/nanoparticle DDSs. Additionally, we elucidate the latest manipulation strategies of microfluidic techniques that impact foundational structures related to the CQAs of micro/nanoparticle DDSs. Furthermore, we offer insights into the industrial applications and challenges microfluidic techniques face in the context of novel micro/nanoparticle DDSs.

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“…are shown in Tables 5 and 6 . 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168…”

Section: Application Of Microfluidic Technology In Ddssunclassified

Section: Micellesmentioning

confidence: 99%

Section: Micellesmentioning

confidence: 99%

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Research Strategies for Precise Manipulation of Micro/Nanoparticle Drug Delivery Systems Using Microfluidic Technology: A Review

Liu,

Fu,

et al. 2024

Pharmaceutical Fronts

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“… 35 Intrinsic factors include the molecular weight and degree of deacetylation of parent chitosan, size and concentration of nanocarriers, etc. 36 The extrinsic factors include pH, temperature, etc. 22 The mechanism includes binding of chitosan to the negatively charged bacterial cell wall, causing altering of the cell surface permeability.…”

Section: Introductionmentioning

confidence: 99%

“…mutants. , Although the exact mechanism of highly efficacious antibacterial activity of chitosan is yet to be understood, it is well known that the antibacterial activity of chitosan is based on a number of intrinsic and extrinsic factors . Intrinsic factors include the molecular weight and degree of deacetylation of parent chitosan, size and concentration of nanocarriers, etc . The extrinsic factors include pH, temperature, etc .…”

Section: Introductionmentioning

confidence: 99%

Efficient Synergistic Antibacterial Activity of α-MSH Using Chitosan-Based Versatile Nanoconjugates

Barman

Chakraborty²,

Saha³

et al. 2023

ACS Omega

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The application of antimicrobial peptides has emerged as an alternative therapeutic tool to encounter against multidrug resistance of different pathogenic organisms. α-Melanocyte stimulating hormone (α-MSH), an endogenous neuropeptide, is found to be efficient in eradicating infection of various kinds of Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus (MRSA). However, the chemical stability and efficient delivery of these biopharmaceuticals (i.e., α-MSH) to bacterial cells with a significant antibacterial effect remains a key challenge. To address this issue, we have developed a chitosan-cholesterol polymer using a single-step, one-pot, and simple chemical conjugation technique, where α-MSH is loaded with a significantly high amount (37.7%), and the final product is obtained as chitosan-cholesterol α-MSH polymer–drug nanoconjugates. A staphylococcal growth inhibition experiment was performed using chitosan-cholesterol α-MSH and individual controls. α-MSH and chitosan-cholesterol both show bacterial growth inhibition by a magnitude of 50 and 79%, respectively. The killing efficiency of polymer–drug nanoconjugates was very drastic, and almost no bacterial colony was observed (∼100% inhibition) after overnight incubation. Phenotypic alternation was observed in the presence of α-MSH causing changes in the cell structure and shape, indicating stress on Staphylococcus aureus. As a further consequence, vigorous cell lysis with concomitant release of the cellular material in the nearby medium was observed after treatment of chitosan-cholesterol α-MSH nanoconjugates. This vigorous lysis of the cell structure is associated with extensive aggregation of the bacterial cells evident in scanning electron microscopy (SEM). The dose-response experiment was performed with various concentrations of chitosan-cholesterol α-MSH nanoconjugates to decipher the degree of the bactericidal effect. The concentration of α-MSH as low as 1 pM also shows significant inhibition of bacterial growth (∼40% growth inhibition) of Staphylococcus aureus. Despite playing an important role in inhibiting bacterial growth, our investigation on hemolytic assay shows that chitosan-cholesterol α-MSH is significantly nontoxic at a wide range of concentrations. In a nutshell, our analysis demonstrated novel antimicrobial activity of nanoparticle-conjugated α-MSH, which could be used as future therapeutics against multidrug-resistant Staphylococcus aureus and other types of bacterial cells.

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CD44 targeted-chondroitin sulfate nanoparticles: Fine-tuning hydrophobic groups to enhance in vitro pH-responsiveness and in vivo efficacy for advanced breast cancer treatment

Azimijou,

Karimi-Soflou,

Karkhaneh

2024

Biomaterials Advances

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Size-adjustable self-assembled nanoparticles through microfluidic platform promotes neuronal differentiation of mouse embryonic stem cells

Cited by 9 publications

References 59 publications

Research Strategies for Precise Manipulation of Micro/Nanoparticle Drug Delivery Systems Using Microfluidic Technology: A Review

Research Strategies for Precise Manipulation of Micro/Nanoparticle Drug Delivery Systems Using Microfluidic Technology: A Review

Efficient Synergistic Antibacterial Activity of α-MSH Using Chitosan-Based Versatile Nanoconjugates

CD44 targeted-chondroitin sulfate nanoparticles: Fine-tuning hydrophobic groups to enhance in vitro pH-responsiveness and in vivo efficacy for advanced breast cancer treatment

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