Size‐based analysis of virus removal filter fouling using fractionated protein aggregates
Keisuke Tsukamoto,
Ryo Hamamoto,
Ryota Oguri
et al.
Abstract:Fouling by protein aggregates reduces virus removal filter performance. In the present study, we investigated the effects of different‐sized protein aggregates on fouling and aggregate retention in order to better understand the fouling mechanisms. Human immunoglobulin G was denatured by heating to produce aggregates of various sizes and then fractionated by size exclusion chromatography into different‐sized aggregates with a narrow size distribution. The fractionated aggregates were filtered on Planova 20N, a… Show more
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