Skeletal stem cell and bone implant interactions are enhanced by LASER titanium modification

Sisti, Karin Ellen; Andrés, María C. de; Johnston, D.; Almeida-Filho, Edson de; Guastaldi, Antônio Carlos; Oreffo, Richard O.C.

doi:10.1016/j.bbrc.2015.10.013

Cited by 23 publications

(20 citation statements)

References 41 publications

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“…A–d) confirming the initiation of osteogenic pathways . Expression of Osteopontin ( OPN ), an important component in bone nodule formation , however, was lower in osteo‐permissive medium with P21‐RUNX2‐8R transduction in comparison to dexamethasone‐induced cells. OSTEOCALCIN (OCN) ( BGLAP , bone gamma‐carboxyglutamic acid‐containing protein), a protein secreted by mature osteoblasts, is significantly upregulated during week 4 in P21‐RUNX2‐8R treated cells cultured in osteo‐permissive medium (Fig.…”

Section: Resultsmentioning

confidence: 88%

Osteogenic Programming of Human Mesenchymal Stem Cells with Highly Efficient Intracellular Delivery of RUNX2

Thiagarajan

Abu-Awwad

Dixon

2017

Stem Cells Translational Medicine

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Mesenchymal stem cells (MSCs) are being exploited in regenerative medicine due to their tri‐lineage differentiation and immunomodulation activity. Currently, there are two major challenges when directing the differentiation of MSCs for therapeutic applications. First, chemical and growth factor strategies to direct osteogenesis in vivo lack specificity for targeted delivery with desired effects. Second, MSC differentiation by gene therapy is difficult as transfection with existing approaches is clinically impractical (viral transfection) or have low efficacy (lipid‐mediated transfection). These challenges can be avoided by directly delivering nonvirally derived recombinant protein transcription factors with the glycosaminoglycan‐binding enhanced transduction (GET) delivery system (P21 and 8R peptides). We used the osteogenic master regulator, RUNX2 as a programming factor due to its stage‐specific role in osteochondral differentiation pathways. Herein, we engineered GET‐fusion proteins and compared sequential osteogenic changes in MSCs, induced by exposure to GET fusion proteins or conventional stimulation methods (dexamethasone and Bone morphogenetic protein 2). By assessing loss of stem cell‐surface markers, upregulation of osteogenic genes and matrix mineralization, we demonstrate that GET‐RUNX2 efficiently transduces MSCs and triggers osteogenesis by enhancing target gene expression directly. The high transduction efficiency of GET system holds great promise for stem cell therapies by allowing reproducible transcriptional control in stem cells, potentially bypassing problems observed with high‐concentration growth‐factor or pleiotropic steroid therapies. Stem Cells Translational Medicine 2017;6:2146–2159

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Section: Resultsmentioning

confidence: 88%

Osteogenic Programming of Human Mesenchymal Stem Cells with Highly Efficient Intracellular Delivery of RUNX2

Thiagarajan

Abu-Awwad

Dixon

2017

Stem Cells Translational Medicine

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“…71 Hence, clinical outcomes echo the uncertain impact high IT might have on peri-implant bone loss compared with low IT. Future research are currently investigating alternative strategies including the application of osseodensification protocols, 72 lasers, 73,74 or ultrasound tools [75][76][77][78] to enhance osseointegration.…”

Section: Implant Outcomes Under High and Low Implant Torquementioning

confidence: 99%

Basis of bone metabolism around dental implants during osseointegration and peri‐implant bone loss

Ínsua

Monje

Wang

et al. 2017

J Biomedical Materials Res

195

188

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Despite the growing number of publications in the field of implant dentistry, there are limited studies to date investigating the biology and metabolism of bone healing around dental implants and their implications in peri-implant marginal bone loss. The aim of this review article is to provide a thorough understanding of the biological events taking place during osseointegration and the subsequent early and late phases of bone remodeling around dental implants. An update on the coupling mechanism occurring during bone resorption-bone remodeling is provided, focused on the relevance of the osteocytes, bone lining cells and immune cells during bone maintenance. An electronic and manual literature search was conducted by three independent reviewers in several databases, including MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, and Cochrane Oral Health Group Trials Register databases for articles up to September 2016 with no language restriction. Local bone metabolism is subject to signals from systemic calcium-phosphate homeostasis and bone remodeling. Three areas of interest were reviewed due to recent reported compromises in bone healing including the putative effects of (1) cholesterol, (2) hyperlipidemia, and (3) low vitamin D intake. Moreover, the prominent influence of osteocytes and immune cells is discussed as being key regulators during dental implant osseointegration and maintenance. These cells are of crucial importance in the presence of biofilm accumulation and their associated byproducts that leads to hard and soft tissue breakdown; the so called peri-implantitis. Factors that could negatively impact osteoclastogenesis or osteal macrophage activation should be monitored in future research including implant placement/torque protocols, bone characteristics, as well as meticulous maintenance programs to favor osseointegration and future long-term stability and success of dental implants. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2075-2089, 2017.

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“…Changes in cell spreading and cytoskeletal tension will alter nucleus shape, chromosomal arrangement, and gene transcription. Hence, changes in cell mechanics directly influence cell gene expression profiles, leading to bone ‐ like nodule formation [41, 42].…”

Section: From Mscs To Obs: Multistep Molecular Eventsmentioning

confidence: 99%

Concise Review: In Vitro Formation of Bone-Like Nodules Sheds Light on the Application of Stem Cells for Bone Regeneration

Alami

Gangloff

Laurent-Maquin

et al. 2016

Stem Cells Translational Medicine

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Harnessing the differentiation of stem cells into bone-forming cells represents an intriguing avenue for the creation of functional skeletal tissues. Therefore, a profound understanding of bone development and morphogenesis sheds light on the regenerative application of stem cells in orthopedics and dentistry. In this concise review, we summarize the studies deciphering the mechanisms that govern osteoblast differentiation in the context of in vitro formation of bone-like nodules, including morphologic and molecular events as well as cellular contributions to mineral nucleation, occurring during osteogenic differentiation of stem cells. This article also highlights the limitations of current translational applications of stem cells and opportunities to use the bone-like nodule model for bone regenerative therapies. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:1587-1593 SIGNIFICANCEHarnessing the differentiation of stem cells into bone-forming cells represents an intriguing avenue for the creation of functional skeletal tissues. Therefore, a profound understanding of bone development and morphogenesis sheds light on the regenerative application of stem cells in orthopedics and dentistry. In this concise review, studies deciphering the mechanisms that govern osteoblast commitment and differentiation are summarized. This article highlights the limitations of current translational applications of stem cells and the opportunities to use the bone-like nodule model for bone regenerative therapies.

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Skeletal stem cell and bone implant interactions are enhanced by LASER titanium modification

Cited by 23 publications

References 41 publications

Osteogenic Programming of Human Mesenchymal Stem Cells with Highly Efficient Intracellular Delivery of RUNX2

Osteogenic Programming of Human Mesenchymal Stem Cells with Highly Efficient Intracellular Delivery of RUNX2

Basis of bone metabolism around dental implants during osseointegration and peri‐implant bone loss

Concise Review: In Vitro Formation of Bone-Like Nodules Sheds Light on the Application of Stem Cells for Bone Regeneration

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