Skin derived precursor Schwann cells improve behavioral recovery for acute and delayed nerve repair

Khuong, Hélène T.; Kumar, Ranjan; Senjaya, Ferry; Grochmal, Joey; Ivanovic, Aleksandra; Shakhbazau, Antos; Forden, Joanne; Webb, Aubrey A.; Biernaskie, Jeffrey; Midha, Rajiv

doi:10.1016/j.expneurol.2014.01.002

Cited by 81 publications

(79 citation statements)

References 55 publications

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“…Muscle weight and CMAPs superior in SKP-SC group in comparison to media injected controls; significantly higher counts of axon regeneration in SKP-SC group equivalent to immediate suture group Walsh et al [22] Rat sciatic transection [122] Rat sciatic and tibial (12 mm gap)…”

Section: Culture Medium Direct Injection Into Distal Nervementioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

129

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Outcomes following peripheral nerve injury remain frustratingly poor. The reasons for this are multifactorial, although maintaining a growth permissive environment in the distal nerve stump following repair is arguably the most important. The optimal environment for axonal regeneration relies on the synthesis and release of many biochemical mediators that are temporally and spatially regulated with a high level of incompletely understood complexity. The Schwann cell (SC) has emerged as a key player in this process. Prolonged periods of distal nerve stump denervation, characteristic of large gaps and proximal injuries, have been associated with a reduction in SC number and ability to support regenerating axons. Cell based therapy offers a potential therapy for the improvement of outcomes following peripheral nerve reconstruction. Stem cells have the potential to increase the number of SCs and prolong their ability to support regeneration. They may also have the ability to rescue and replenish populations of chromatolytic and apoptotic neurons following axotomy. Finally, they can be used in non-physiologic ways to preserve injured tissues such as denervated muscle while neuronal ingrowth has not yet occurred. Aside from stem cell type, careful consideration must be given to differentiation status, how stem cells are supported following transplantation and how they will be delivered to the site of injury. It is the aim of this article to review current opinions on the strategies of stem cell based therapy for the augmentation of peripheral nerve regeneration.

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Section: Culture Medium Direct Injection Into Distal Nervementioning

confidence: 99%

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Fairbairn

Meppelink

Ng-Glazier

et al. 2015

WJSC

129

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“…Present time points for the regeneration analysis were based on our earlier observations of peripheral nerve regeneration after transection injury. 19,20,47 In sharp contrast to the medium injection group, animals that received cell therapy showed by Week 10 clear signs of return to the normalgesic state according to a standard Hargreaves plantar test. The SKP-SC group showed maximum recovery potential, which was, however, closely followed by the nSC group.…”

Section: Discussionmentioning

confidence: 99%

“…Assessment of skilled locomotion using the ladder-rung technique 19,27,28 also did not reveal a significant additive effect of cell therapy after sciatic nerve transection, but we found a noticeable improvement with SKP-SC treatment in a tibial nerve repair model. 20 Observed differences in recovery between small and large peripheral nerves may lead to a better understanding of regenerative potential span for stem cell therapies.…”

mentioning

confidence: 99%

Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells

Shakhbazau

Mohanty²,

Kumar

et al. 2014

JNS

Self Cite

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Object Cell therapy is a promising candidate among biological or technological innovations sought to augment microsurgical techniques in peripheral nerve repair. This report describes long-term functional regenerative effects of cell therapy in the rat injury model with a focus on sensory recovery. Methods Schwann cells were derived from isogenic nerve or skin precursor cells and injected into the transected and immediately repaired sciatic nerve distal to the injury site. Sensory recovery was assessed at weeks 4, 7, and 10. Axonal regeneration was assessed at Week 11. Results By Week 10, thermal sensitivity in cell therapy groups returned to a level indistinguishable from the baseline (p > 0.05). Immunohistochemistry at 11 weeks after injury showed improved regeneration of NF+ and IB4+ axons. Conclusions: The results of this study show that cell therapy significantly improves thermal sensation and the number of regenerated sensory neurons at 11 weeks after injury. These findings contribute to the view of skin-derived stem cells as a reliable source of Schwann cells with therapeutic potential for functional recovery in damaged peripheral nerve.

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“…For instance, precursor skin derived stem cells used in combination with tubular constructs have been shown to enhance the repair across both sciatic and tibial nerves in the rat [105][106][107] .…”

Section: Adipose Tissue Derived Mesenchymal Stem Cellsmentioning

confidence: 99%

“…A wide variety of stem cells have been investigated for their use in peripheral nerve repair including neural stem cells 92 , mesenchymal stem cells -bone marrow and adipose tissue derived 96,101 , skin stem cells 105 , embryonic stem cells 109 , induced pluripotent stem cells 111 , and genetically modified cells . Although differences exist in stem cell origin, they are all used as alternatives to ASCs.…”

Section: Stem Cells As An Alternative To Ascs For Enhanced Repairmentioning

confidence: 99%

Advances in Nerve Guidance Conduit-Based Therapeutics for Peripheral Nerve Repair

Lackington

Ryan

O’Brien

2017

ACS Biomater. Sci. Eng.

103

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Skin derived precursor Schwann cells improve behavioral recovery for acute and delayed nerve repair

Cited by 81 publications

References 55 publications

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Augmenting peripheral nerve regeneration using stem cells: A review of current opinion

Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells

Advances in Nerve Guidance Conduit-Based Therapeutics for Peripheral Nerve Repair

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