Skin Grafting: Dermal Components in Inhibition of Wound Contraction

Brown, David M.; Garner, W.L.; Vl, Young

doi:10.1097/00007611-199007000-00018

Cited by 32 publications

(19 citation statements)

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“…21,25,26 It has been shown to be dependent from the type of grafts and from the type of dressing as well as the provision of topical nutrients. [21][22][23]26,38 Wound contraction rates have been similar in other experimental studies by our group when using pure epithelial transplants, 17a whereas in combination with dermal elements a significantly reduced contraction rate could be found. 17b So far, the contraction rates found in this study are within the expected range for this more or less merely epithelial type of grafting.…”

Section: Discussionsupporting

confidence: 83%

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Cultured Human Keratinocytes on Type I Collagen Membranes to Reconstitute the Epidermis

Horch

Debus²,

Wagner³

et al. 2000

Tissue Engineering

114

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The development of new techniques and modifications to overcome some of the disadvantages in cultured keratinocyte grafting has been motivated by several well-known drawbacks in the use of cultured epithelial autografts such as long culture periods, lack of adherence, difficulty in handling, lack of dermal substrates, and high costs. Two recent insights have influenced further research. On the one hand, it has been shown that the use of undifferentiated proliferative cells in fibrin glue suspensions is effective in epithelial reconstitution. On the other hand, the enzymatic release of cells from the culture surfaces is a critical step leading to at least temporary destruction of anchoring structures of the cultured cells. In this study, we tried to combine these two aspects in an attempt to modify common modalities of keratinocyte transplantation. To avoid dispase dissolving of the cultured cells, keratinocytes were seeded onto bovine collagen type I membranes without feeder layers and under serum-free culture conditions. Subconfluent monolayers of cultured human keratinocytes were transplanted as an upside-down graft on collagen membranes (keratinocyte collagen membrane grafts [KCMG], n = 12) after 3 days of culture or as membrane grafts alone (n = 12) onto standard nude mice full-thickness wounds. Fully differentiated epidermis was found at 21 days after grafting KCMG with persistence of human keratinocytes. This study demonstrates that upside-down grafts of undifferentiated monolayers of keratinocytes on non-cross-linked bovine type I collagen membranes do lead to an early reconstitution of multilayered squamous epithelium with enhanced wound healing compared to the control group. The upside down KCMG grafting technique is able to transfer actively proliferative keratinocytes and simplifies the application compared to conventional epithelial sheet grafting.

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Section: Discussionsupporting

confidence: 83%

“…The shrinkage of experimental fullthickness rodent wounds 38 and nude mouse wounds has been described by others. 21,25,26 It has been shown to be dependent from the type of grafts and from the type of dressing as well as the provision of topical nutrients.…”

Section: Discussionmentioning

confidence: 77%

Cultured Human Keratinocytes on Type I Collagen Membranes to Reconstitute the Epidermis

Horch

Debus²,

Wagner³

et al. 2000

Tissue Engineering

114

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“…7,9,10 The results of the Vancouver Scar Scale showed similar pliability in both groups. Another advantage of the dermis graft is the quick healing of the donor site with no obvious scar, because the epidermis can be replaced immediately to ensure closure of the donor bed.…”

Section: Discussionmentioning

confidence: 81%

Dermis Graft for Wound Coverage

Han

Yoon

Kim

et al. 2007

Plastic and Reconstructive Surgery

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“…Regarding wound contraction of the recipient site after dermis grafting, the contraction of myofibroblasts can be inhibited by grafting with a more dermal portion than a regular skin graft. In previous studies, the inhibition of scar contraction was attributed to the amount of dermal collagen rather than to the amount of epidermis (Chou et al, 2001;Rudolph, 1979;Brown et al, 1990). Another advantage of the dermis graft is the quick healing of the donor site without an obvious scar appearance because the epidermis can be replaced immediately to ensure the closure of the donor bed.…”

Section: Advantages and Disadvantagesmentioning

confidence: 99%

Efficacy of Autogenous Dermis Graft for Wound Coverage

Han¹

2011

Skin Grafts - Indications, Applications and Current Research

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Skin Grafting: Dermal Components in Inhibition of Wound Contraction

Cited by 32 publications

References 0 publications

Cultured Human Keratinocytes on Type I Collagen Membranes to Reconstitute the Epidermis

Cultured Human Keratinocytes on Type I Collagen Membranes to Reconstitute the Epidermis

Dermis Graft for Wound Coverage

Efficacy of Autogenous Dermis Graft for Wound Coverage

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