Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits

Lorenz, Yvonne; Enrique, Ernesto; LeQuynh, Lien; Fötisch, Kay; Retzek, Mechthild; Biemelt, Sophia; Sonnewald, Uwe; Vieths, Stefan; Scheurer, Stephan

doi:10.1016/j.jaci.2006.05.014

Cited by 55 publications

(27 citation statements)

References 25 publications

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“…This may someday be a plausible approach, because RNA interference techniques were successfully used to create less allergenic forms of tomato by silencing expression of profilin 28 or lipid transfer protein. 29 Eosinophilic esophagitis is typically a food-responsive gastrointestinal disease, but food elimination is often an unsatisfactory treatment because numerous foods are commonly implicated, resulting in frustrating dietary trials and socially and nutritionally restrictive treatment diets. 30 Stein et al 31 undertook an open-label phase I/II study of a humanized monoclonal IgG 1 antibody against IL-5 in 4 adults with eosinophilic esophagitis.…”

Section: Treatmentmentioning

confidence: 99%

Advances in allergic skin disease, anaphylaxis, and hypersensitivity reactions to foods, drugs, and insects

Sicherer

Leung

2007

Journal of Allergy and Clinical Immunology

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This review highlights some of the research advances in anaphylaxis and hypersensitivity reactions to foods, drugs, and insects and in allergic skin disease that were reported primarily in the Journal in 2006. Advances in diagnosis include identification of food proteins to which IgE binding is associated with severe reactions; elucidation of diagnostic relationships of skin prick test wheal size with outcomes of egg, tree nut, and sesame allergy; evaluation of the diagnostic utility of atopy patch testing for food; and the observation that yellow jacket sting outcomes are influenced by species. Mechanistic observations include the following: heating of birch pollen-related foods disrupts IgE binding but not T-cell epitopes; a simple imbalance of T(H)1/T(H)2 response does not explain variations in clinical expression of peanut allergy; and elucidation of the role of dendritic cells in drug hypersensitivity. With regard to treatment, a rapidly disintegrating epinephrine tablet showed promise for sublingual treatment of anaphylaxis, RNA interference techniques showed promise in creating lower-allergenic foods, and anti-IL-5 showed promise for treatment of eosinophilic esophagitis. Progress in our understanding of the immunology and the etiology of skin barrier dysfunction in atopic dermatitis has also been made. These observations will likely contribute toward optimizing management of these common allergic disorders.

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Section: Treatmentmentioning

confidence: 99%

Advances in allergic skin disease, anaphylaxis, and hypersensitivity reactions to foods, drugs, and insects

Sicherer

Leung

2007

Journal of Allergy and Clinical Immunology

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“…The practical value of LTPs has even developed to the extent that hypoallergenic tomatoes have been developed through suppressing the expression of LTP genes in transgenic fruit (Le et al 2006;Lorenz et al 2006), yet there is still no generalized theory as to their in vivo function. In recent years, plant genome sequencing efforts have revealed LTP and ''LTP-like'' sequences to comprise large, ubiquitous gene families in higher plants, and the stage is therefore set to elucidate the breadth of LTP functional diversity.…”

mentioning

confidence: 99%

The biochemistry and biology of extracellular plant lipid‐transfer proteins (LTPs)

2008

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Plant lipid-transfer proteins (LTPs) are abundant, small, lipid binding proteins that are capable of exchanging lipids between membranes in vitro. Despite their name, a role in intracellular lipid transport is considered unlikely, based on their extracellular localization. A number of other biological roles, including antimicrobial defense, signaling, and cell wall loosening, have been proposed, but conclusive evidence is generally lacking, and these functions are not well correlated with in vitro activity or structure. A survey of sequenced plant genomes suggests that the two biochemically characterized families of LTPs are phylogenetically restricted to seed plants and are present as substantial gene families. This review aims to summarize the current understanding of LTP biochemistry, as well as the evidence supporting the proposed in vivo roles of these proteins within the emerging post-genomic framework.

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“…Natural breeding and breeding in combination with random mutation strategies [38,39,40] accompanied by serological analysis may allow us to foster effectively viable hypoallergenic cultivars. Several attempts have been made so far to silence the expression of certain allergens in rice, grass pollen, apple and tomato by anti-sense technologies [23,24,25,26,27,28,29]. Using this technology it has been possible to down-regulate the expression of certain allergens but it may affect growth and viability of the engineered plants.…”

Section: Discussionmentioning

confidence: 99%

“…Preventative research has included silencing allergenic proteins in plants by anti-sense technology, which has been used to reduce the expression of certain allergens in rice, grass pollen, apples and tomatoes, with the aim of reducing their allergenic activity [23,24,25,26,27,28,29]. …”

Section: Introductionmentioning

confidence: 99%

Detection of Antigens Reactive to IgE and IgA during Wheat Seed Maturation and in Different Wheat Cultivars

Constantin¹,

Touraev

Heberle‐Bors

et al. 2009

Int Arch Allergy Immunol

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Background: Dietary intake of wheat causes hypersensitivity reactions in patients suffering from IgE-mediated food allergy and coeliac disease. Aim: To study the expression of IgE- and IgA-reactive antigens during wheat seed maturation and in different wheat cultivars. Methods: Summer wheat was grown in a glasshouse and seeds were harvested at defined maturation stages. Mature seeds were obtained from 13 different defined cultivars. Protein extracts were prepared from different maturation stages and cultivars with a standardized procedure based on seed weight and analysed by IgE and IgA immunoblotting using sera from clinically defined patients suffering from wheat allergy or coeliac disease. Results: With a few exceptions the expression of IgE- and IgA-reactive wheat antigens increased during wheat seed maturation. Wheat cultivars could be identified in which the expression of certain IgE- and IgA-reactive components was strongly reduced or not detectable. Conclusions: The expression of IgE- and IgA-reactive antigens depends on wheat seed maturation and varies in different wheat cultivars.

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Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits

Cited by 55 publications

References 25 publications

Advances in allergic skin disease, anaphylaxis, and hypersensitivity reactions to foods, drugs, and insects

Advances in allergic skin disease, anaphylaxis, and hypersensitivity reactions to foods, drugs, and insects

The biochemistry and biology of extracellular plant lipid‐transfer proteins (LTPs)

Detection of Antigens Reactive to IgE and IgA during Wheat Seed Maturation and in Different Wheat Cultivars

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