SkinEthic™ HCE Eye Irritation Test: Similar performance demonstrated after long distance shipment and extended storage conditions

Leblanc, Virginie; Yokota, Mariko; Grandidier, Marie-Hélène; Yoshida, Daisuke; Adriaens, Els; Cotovio, José; Kyoutani, Daiki; Alépée, Nathalie

doi:10.1016/j.tiv.2018.10.003

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…This system consists of immortalized human corneal epithelial cells in a chemically defined medium that structurally resembles the corneal mucosa of the human eye [ 27 , 28 ]. Percent viability is quantified after a single chemical exposure based on the MTT assay and compared with an unexposed control [ 29 ]. Like the EpiOcular™ system discussed above, the HCE model is also incapable of assigning substances to Category 1 or Category 2 of the GHS [ 29 , 30 ].…”

Section: Assessments Of Ocular Toxicitymentioning

confidence: 99%

“…Percent viability is quantified after a single chemical exposure based on the MTT assay and compared with an unexposed control [ 29 ]. Like the EpiOcular™ system discussed above, the HCE model is also incapable of assigning substances to Category 1 or Category 2 of the GHS [ 29 , 30 ]. Despite this constraint, a viability above 60% after exposure to a liquid or a viability above 50% after exposure to a solid is designated “No Category”, or non-irritation [ 29 , 31 ].…”

Section: Assessments Of Ocular Toxicitymentioning

confidence: 99%

“…Like the EpiOcular™ system discussed above, the HCE model is also incapable of assigning substances to Category 1 or Category 2 of the GHS [ 29 , 30 ]. Despite this constraint, a viability above 60% after exposure to a liquid or a viability above 50% after exposure to a solid is designated “No Category”, or non-irritation [ 29 , 31 ]. The SkinEthic™ HCE model is utilized to evaluate the raw materials and products of cosmetic, chemical, and pharmaceutical companies [ 25 ].…”

Section: Assessments Of Ocular Toxicitymentioning

confidence: 99%

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Toxic External Exposure Leading to Ocular Surface Injury

Rauchman

Locke

Albert

et al. 2023

Vision

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The surface of the eye is directly exposed to the external environment, protected only by a thin tear film, and may therefore be damaged by contact with ambient particulate matter, liquids, aerosols, or vapors. In the workplace or home, the eye is subject to accidental or incidental exposure to cleaning products and pesticides. Organic matter may enter the eye and cause infection. Ocular surface damage can trigger a range of symptoms such as itch, discharge, hyperemia, photophobia, blurred vision, and foreign body sensation. Toxin exposure can be assessed clinically in multiple ways, including via measurement of tear production, slit-lamp examination, corneal staining, and conjunctival staining. At the cellular level, environmental toxins can cause oxidative damage, apoptosis of corneal and conjunctival cells, cell senescence, and impaired motility. Outcomes range from transient and reversible with complete healing to severe and sight-compromising structural changes. Classically, evaluation of tolerance and safety was carried out using live animal testing; however, new in vitro and computer-based, in silico modes are superseding the gold standard Draize test. This review examines how environmental features such as pollutants, temperature, and seasonality affect the ocular surface. Chemical burns to the eye are considered, and approaches to protect the ocular surface are detailed.

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