Skullcapflavone I has a potent anti‐pancreatic cancer activity by targeting miR‐23a

Abstract

Baicalein has been widely studied and showed a potent activity against pancreatic cancer in both in vivo and in vitro studies. Little is known regarding the effects of Skullcapflavone I (SFI), despite they have similar structures. So, this study was to explore the function of SFI on human pancreatic cancer. Panc-1 cells were transfected with miR-23a precursor, miR-23a inhibitor or the negative controls, and subsequently treated by SFI. Cell viability, Bromodeoxyuridine (BrdU)-positive cell rate, apoptosis, migration, invasion, and related protein expression were assessed by utilizing Cell Counting Kit-8 (CCK-8), BrdU staining, apoptosis assessment, transwell assay, and western blot. SFI significantly reduced the proliferation, migration, and invasion, as well as induced apoptosis of Panc-1 cells. MiR-23a, miR-21, and miR-155 were lowly expressed while miR-145 and miR-146a were highly expressed in SFI-treated cell. Of note, the antitumor effects of SFI were promoted by miR-23a suppression whereas attenuated by miR-23a overexpression. JAK/STAT and MAPK pathways were inhibited by SFI. Also, the pathway inhibition in SFI-treated cells was reversed by miR-23a overexpression. SFI might be a promising anti-pancreatic cancer agent by inhibiting cancer cells growth and motility. The anticancer activities of SFI might be through downregulation of miR-23a, as well as inhibition of JAK/STAT and MAPK pathways. K E Y W O R D S miR-23a, Panc-1 cell, pancreatic cancer, skullcapflavone I 1 | INTRODUCTION Pancreatic cancer, as one of the deadly diseases, has a 5-year survival rate of <7%. 1 As the rate of obesity, hyperlipidemia, diabetes, and chronic pancreatitis has exploded, the prevalence of pancreatic cancer is rising. In 2012, approximately 338,000 people had pancreatic cancer worldwide. 2 Pancreatic cancer is difficult to be diagnosed in an early stage since no symptoms usually appear; thus, a majority of patients present with

Retracted: Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation

“…Other studies pointed out circ0009910 enhanced carcinoma via the JAK1/STAT3 pathway (Deng et al, ) and circRNA0006528 enhanced breast carcinoma via the MEK/ERK pathway (Gao et al, ). Moreover, research pointed out miR‐23a exerted efficacies in pancreatic carcinoma via JAK/STAT pathway (Cui et al, ) and miR‐520c exerted efficacies in fibrosarcoma via MEK/ERK signaling pathway (Liu & Wilson, ). Fortunately, we also discovered that overexpression of circFNDC3B enhanced the ratio of p/t‐JAK1, p/t‐STAT3, p/t‐MEK, and p/t‐ERK in RC cells.…”

Network pharmacology reveals potential functional components and underlying molecular mechanisms of Andrographis paniculata in esophageal cancer treatment

“…While the pharmacological properties of most of these compounds have not been characterized, the antitumor/antiinflammatory properties of panicolin, moslosooflavone and deoxyandrographiside have been documented. Panicolin, also known as skullcapflavone I, has been shown to reduce the proliferation, migration and invasion, as well as induce apoptosis of human pancreatic cells via downregulation of miR‐23a expression and inhibition of the JAK/STAT and MAPK pathways (Cui et al, 2020). Panicolin isolated from AP has been reported to inhibit the production of key inflammatory mediators nitric oxide, prostaglandin E2 and IL‐6 in murine macrophages, and leukotriene B4 and thromboxane B2 in human leukemia cells (Chandrasekaran et al, 2011).…”

CircRNAs as Novel Biomarkers and Therapeutic Targets in Renal Cell Carcinoma

“…However, circNRIP1 plays an oncogenic role in RCC, which activates adenylate-activated protein kinase (AMPK) and PI3K/AKT/mTOR pathways by targeting miR-505 (Dong et al, 2020). In recent years, the Janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and extracellular signal-regulated kinase (ERK) kinase (MEK)/ERK signaling pathways have been widely reported to involve in cancer development (Kang et al, 2011;Deng et al, 2018;Gao et al, 2019;Cui et al, 2020). It has also been proved that some circRNAs play vital roles in the development of RCC by affecting these pathways.…”