SL15: A seminal plasma‐derived lectin from the sperm of llama (<i>Lama glama</i>)

Zampini, Renato; Sequeira, Sabrina; Argañaraz, Martin Eduardo; Apichela, Silvana Andrea

doi:10.1002/mrd.22816

Cited by 10 publications

(5 citation statements)

References 41 publications

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“…Supporting this hypothesis, β‐NGF was detected abundantly in SAP fraction, easily removed by 0.5 M KCl. This technique is consistent with descriptions for SAP in llama (Zampini, Sequeira, Argañaraz, & Apichela, ) and other livestock (e.g., bulls (Einspanier et al, ; Manjunath, Bergeron, Lefebvre, & Fan, ), rams (Bergeron, Villemure, Lazure, & Manjunath, ; Pérez‐Pé, Cebrián‐Pérez, & Muiño‐Blanco, ), and boars (Garénaux et al, )).…”

Section: Discussionsupporting

confidence: 86%

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Expression of β‐NGF and high‐affinity NGF receptor (TrKA) in llama (Lama glama) male reproductive tract and spermatozoa

Sari

Zampini

Argañaraz

et al. 2018

Molecular Reproduction Devel

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β‐Nerve growth factor (β‐NGF) is a seminal plasma element, responsible for inducing ovulation in camelids. The main organ of β‐NGF production remains nondescript. The aims of this study were to (a) characterize gene expression and protein localization of β‐NGF and its main receptor tyrosine kinase receptor A (TrKA) in the llama male reproductive tract, and (b) determine whether the seminal β‐NGF interacts with ejaculated sperm by localizing β‐NGF and TrKA in epididymal, ejaculated, and acrosome‐reacted (AR) sperms and, additionally, by identifying β‐NGF presence in sperm‐adsorbed proteins (SAP). Both β‐NGF and TrkA transcripts are widely expressed along the male reproductive tract, with a higher expression level of β‐NGF at prostate (p < 0.05). β‐NGF immunolabeling was only positive for prostate, whereas TrKA label was present in epithelial and muscular cells of testis, prostate, bulbourethral glands, and epididymis. Using an immunofluorescent technique, β‐NGF was colocalized with TrKA in the middle piece of ejaculated and AR sperm. However, only TrKA was observed in epididymal sperm indicating that β‐NGF could have a seminal origin. This was also confirmed by the identification of four β‐NGF isoforms in SAP. This study extends the knowledge about the participation of β‐NGF/TrkA in llama reproduction, providing evidence that may have roles in the regulation of sperm physiology.

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Section: Discussionsupporting

confidence: 86%

“…SAPs were obtained as previously described by Zampini et al (). Briefly, pools of three semen samples were diluted fivefold and washed three times with HBSS (25 mM Hepes, 130 mM NaCl, 5 mM KCl, 0.36 mM NaH 2 PO 4 , 0.49 mM MgCl 2 , and 2.4 mM CaCl 2 ; pH 7.4, 290 mOsm/kg) to remove SP.…”

Section: Methodsmentioning

confidence: 99%

Expression of β‐NGF and high‐affinity NGF receptor (TrKA) in llama (Lama glama) male reproductive tract and spermatozoa

Sari

Zampini

Argañaraz

et al. 2018

Molecular Reproduction Devel

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“…In addition, Sari et al (2018) determined that the β‐NGF could exert a role in llama sperm physiology by confirming with histochemistry the presence of its receptor (TrKA) both in epididymal and ejaculated spermatozoon. Recently, Zampini, Sequeria, Argañaraz, and Apichela (2017) determined the presence of the SL15 protein, of the family of lectins, in llama SP. This protein could be involved in the formation of the sperm reservoir as it binds to the N ‐acetylgalactosamine present in the oviductal epithelium (Apichela et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Incubation of frozen‐thawed llama sperm with seminal plasma

et al. 2020

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Seminal plasma is intimately connected to sperm physiology and particularly in South American Camelids, has demonstrated to be involved in multiple physiological reproductive events. Different percentages of seminal plasma (0%, 10% and 50%) were added to thawed llama semen samples with the objective of evaluating the interaction with cryopreserved sperm over time (0, 1.5 and 3 hr at 37°C). A total of 20 ejaculates from five adult llama males (n = 5; r = 4) were evaluated. A significant decrease in sperm motility, membrane function and live sperm was observed in all thawed samples (0%, 10% and 50%) at 0 hr when compared to raw semen. Neither morphology nor chromatin condensation was altered in all thawed samples (p > .05), but a significant increase in the percentage of spermatozoa with fragmented DNA was observed after thawing all samples versus raw semen. When evaluating thawed samples over time, a significant decrease of motility and membrane function was observed, while the percentages of total live sperm were preserved over the 3 hr of incubation in all final concentrations evaluated. To conclude, the addition of 10% or 50% of seminal plasma was incapable of preserving motility or membrane function of frozen‐thawed llama sperm during 3 hr of incubation.

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“…An ovulation‐inducing protein factor (NGF‐ß) has been described (Adams, Ratto, Silva, & Carrasco, ), that not only induces ovulation by stimulating pituitary LH secretion but also adheres to the sperm membrane (Sari et al, ). On the other hand, a llama seminal lectin (SL15) that is likely adsorbed to spermatozoa via SP during ejaculation and is probably involved in sperm reservoir formation (Zampini, Sequeria, Argañaraz, & Apichela, ). In other species, some components of SP have been shown to act as protective agents against premature capacitation, which would seem to indicate the need to add a certain percentage of SP to cryopreservation diluents (Du et al, ; Kershaw‐Young & Maxwell, ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the cryoprotective effect of seminal plasma on llama ( Lama glama) spermatozoa

et al. 2019

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In South American camelids, sperm survival is low after thawing and poor results are obtained when artificial insemination is performed with cryopreserved semen. The aim of this study was to evaluate the effect of different percentages (10% and 50%) of seminal plasma added prior to the process of cryopreservation and also to evaluate the absence of seminal plasma on llama sperm survival after freezing and thawing. A total of 15 ejaculates from five adult llama males (n = 5; r = 3) were evaluated. A significant decrease in sperm motility, viability, membrane function and intact acrosomes was observed in thawed samples (0%, 10% and 50%) when compared to raw semen. Neither morphology nor chromatin condensation was altered in all thawed samples (p > 0.05), but a significant increase (p < 0.05) in the percentage of spermatozoa with fragmented DNA was observed after thawing all samples compared to raw semen. Higher percentages of total and progressive sperm motility were observed when 0% and 10% of seminal plasma were used compared to 50%. However, no statistical differences were established for sperm viability, membrane function, morphology, acrosome status and DNA quality between thawed treatments. To conclude, neither of the percentages of seminal plasma used showed superiority nor cryoprotective effect on llama sperm survival.

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SL15: A seminal plasma‐derived lectin from the sperm of llama (Lama glama)

Cited by 10 publications

References 41 publications

Expression of β‐NGF and high‐affinity NGF receptor (TrKA) in llama (Lama glama) male reproductive tract and spermatozoa

Expression of β‐NGF and high‐affinity NGF receptor (TrKA) in llama (Lama glama) male reproductive tract and spermatozoa

Incubation of frozen‐thawed llama sperm with seminal plasma

Evaluation of the cryoprotective effect of seminal plasma on llama ( Lama glama) spermatozoa

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