SlDREB2 gene specifically recognizing to the universal DRE elements is a transcriptional activator improving drought tolerance in tomato

Tao, L. L.; Yu, Guoliang; Hongna, Chen; Wang, Bing; Jiang, Linlin; Han, Xianpei; Lin, Guolin; Cheng, Xuesen

doi:10.1016/j.scienta.2022.110887

Cited by 11 publications

(3 citation statements)

References 52 publications

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“…Similarly, up-regulation of PcDREB2A could significantly improve drought tolerance of Arabidopsis [ 46 ]. On the contrary, RNAi-tomato plants exhibited a significantly lower expression level of SlDREB2 and higher lipid membrane peroxidation than the wild-type under drought stress [ 47 ]. During seed germination, significant increases in expression levels of different types of DREBs are also propitious to achieve stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

Seed Priming with Chitosan Improves Germination Characteristics Associated with Alterations in Antioxidant Defense and Dehydration-Responsive Pathway in White Clover under Water Stress

Ling

Zhao

Cheng

et al. 2022

Plants

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Water stress decreases seed-germination characteristics and also hinders subsequent seedling establishment. Seed priming with bioactive compounds has been proven as an effective way to improve seed germination under normal and stressful conditions. However, effect and mechanism of seed priming with chitosan (CTS) on improving seed germination and seedling establishment were not well-understood under water-deficit conditions. White clover (Trifolium repens) seeds were pretreated with or without 5 mg/L CTS before being subjected to water stress induced by 18% (w/v) polyethylene glycol 6000 for 7 days of germination in a controlled growth chamber. Results showed that water stress significantly decreased germination percentage, germination vigor, germination index, seed vigor index, and seedling dry weight and also increased mean germination time and accumulation of reactive oxygen species, leading to membrane lipid peroxidation during seed germination. These symptoms could be significantly alleviated by the CTS priming through activating superoxide dismutase, catalase, and peroxidase activities. In addition, seeds pretreated with CTS exhibited significantly higher expression levels of genes encoding dehydration-responsive transcription factors (DREB2, DREB4, and DREB5) and dehydrins (Y2K, Y2SK, and SK2) than those seeds without the CTS priming. Current findings indicated that the CTS-induced tolerance to water stress could be associated with the enhancement in dehydration-responsive pathway during seed germination.

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Section: Discussionmentioning

confidence: 99%

Seed Priming with Chitosan Improves Germination Characteristics Associated with Alterations in Antioxidant Defense and Dehydration-Responsive Pathway in White Clover under Water Stress

Ling

Zhao

Cheng

et al. 2022

Plants

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“…Particularly, drought, exogenous ABA, salt and trehalose are also known to regulate the expression of SlDREB1 (Jiang et al, 2017;Rai et al, 2019;Yu et al, 2019). Moreover, it is recognized that SlDREB2 gene plays an important role in orchestrating the expression of stressresistant and functional genes, improving tolerance to water deficit in plants (Tao et al, 2022). In our study the inoculation of PGPB affected the expression of both SlDREB1 and SlDREB2 in plants subjected to water deficit.…”

Section: Impact Of the Bacterial Inoculation On The Expression Of Gen...mentioning

confidence: 54%

Diverse plant promoting bacterial species differentially improve tomato plant fitness under water stress

Zampieri,

Franchi,

Giovannini

et al. 2023

Front. Plant Sci.

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IntroductionFood crops are increasingly susceptible to the challenging impacts of climate change, encompassing both abiotic and biotic stresses, that cause yield losses. Root-associated microorganisms, including plant growth-promoting bacteria (PGPB), can improve plant growth as well as plant tolerance to environmental stresses. The aims of this work were to characterize bacteria isolated from soil and roots of tomato plants grown in open field.MethodsBiochemical and molecular analyses were used to evaluate the PGP potential of the considered strains on tomato plants in controlled conditions, also assessing their effects under a water deficit condition. The isolated strains were classified by 16S gene sequencing and exhibited typical features of PGPB, such as the release of siderophores, the production of proteases, and phosphorous solubilization. Inoculating tomato plants with eleven selected strains led to the identification of potentially interesting strains that increased shoot height and dry weight. Three strains were then selected for the experiment under water deficit in controlled conditions. The tomato plants were monitored from biometric and physiological point of view, and the effect of inoculation at molecular level was verified with a targeted RT-qPCR based approach on genes that play a role under water deficit condition.ResultsResults revealed the PGP potential of different bacterial isolates in tomato plants, both in well-watered and stressed conditions. The used integrated approach allowed to obtain a broader picture of the plant status, from biometric, eco-physiological and molecular point of view. Gene expression analysis showed a different regulation of genes involved in pathways related to abscisic acid, osmoprotectant compounds and heat shock proteins, depending on the treatments. DiscussionOverall, results showed significant changes in tomato plants due to the bacterial inoculation, also under water deficit, that hold promise for future field applications of these bacterial strains, suggesting that a synergistic and complementary interaction between diverse PGPB is an important point to be considered for their exploitation.

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“…Inter-kingdom communication causes the upregulation of drought-response marker genes such as DREB1B-like , ERD15 ( Naylor and Coleman-Derr, 2018 ), and DREB2A ( Sarma and Saikia, 2014 ). The molecular mechanism underlying the plant–PGPR interactions has deepened thanks to advances in molecular techniques, such as the analysis of differential gene expression using cDNA microarrays ( van Loon, 2007 ), qPCR ( Ibort et al., 2018 ), and RNA-seq ( Tao et al., 2022 ). Recent molecular studies have shown that PGPR affects the expression patterns of stress-responsive genes in plants, alleviating the damaging effects of drought stress ( Vurukonda et al., 2016 ; Gupta et al., 2021 ; Manjunatha et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Meta-analysis of plant growth-promoting rhizobacteria interaction with host plants: implications for drought stress response gene expression

Ferrante,

Campagni,

Vettori

et al. 2024

Front. Plant Sci.

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IntroductionThe molecular and physiological mechanisms activated in plants during drought stress tolerance are regulated by several key genes with both metabolic and regulatory roles. Studies focusing on crop gene expression following plant growth-promoting rhizobacteria (PGPR) inoculation may help understand which bioinoculant is closely related to the induction of abiotic stress responses.MethodsHere, we performed a meta-analysis following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines to summarise information regarding plant-PGPR interactions, focusing on the regulation of nine genes involved in plant drought stress response. The literature research yielded 3,338 reports, of which only 41 were included in the meta-analysis based on the chosen inclusion criteria. The meta-analysis was performed on four genes (ACO, APX, ACS and DREB2); the other five genes (ERD15, MYB, MYC, acdS, WRKY) had an insufficient number of eligible articles.ResultsForest plots obtained through each meta-analysis showed that the overexpression of ACO, APX, ACS and DREB2 genes was not statistically significant. Unlike the other genes, DREB2 showed statistically significant results in both the presence and absence of PGPR. Considering I2>75 %, the results showed a high heterogeneity among the studies included, and the cause for this was examined using subgroup analysis. Moreover, the funnel plot and Egger’s test showed that the analyses were affected by strong publication bias.DiscussionThis study argues that the presence of PGPR may not significantly influence the expression of drought stress response-related crop genes. This finding may be due to high heterogeneity, lack of data on the genes examined, and significant publication bias.

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SlDREB2 gene specifically recognizing to the universal DRE elements is a transcriptional activator improving drought tolerance in tomato

Cited by 11 publications

References 52 publications

Seed Priming with Chitosan Improves Germination Characteristics Associated with Alterations in Antioxidant Defense and Dehydration-Responsive Pathway in White Clover under Water Stress

Seed Priming with Chitosan Improves Germination Characteristics Associated with Alterations in Antioxidant Defense and Dehydration-Responsive Pathway in White Clover under Water Stress

Diverse plant promoting bacterial species differentially improve tomato plant fitness under water stress

Meta-analysis of plant growth-promoting rhizobacteria interaction with host plants: implications for drought stress response gene expression

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