SlHY5 is a necessary regulator of the cold acclimation response in tomato

Han, Nani; Fan, Shuya; Zhang, Tiantian; Sun, Haiguo; Zhu, Yongxing; Gong, Haijun; Guo, Jia

doi:10.1007/s10725-020-00583-7

Cited by 31 publications

(19 citation statements)

References 58 publications

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“…Each experiment was repeated at least three times. The relative fold change was calculated by following the ddCT method using actin as normalization reference control (Qiu et al 2016; Han et al 2020; Yan et al 2020). The primers used in this study are listed in Supplementary Table S1.…”

Section: Methodsmentioning

confidence: 99%

Transcriptional regulation ofHSFA7and post-transcriptional modulation ofHSFB4aby miRNA4200 govern general and varietal thermotolerance in tomato

Rao

Balyan

Verma

et al. 2021

Preprint

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Heat shock factors (HSFs) are at the core of heat stress (HS) response in plants. However, the contribution of HSFs governing the inherent thermotolerance mechanism in tomato from sub-tropical hot climates is poorly understood. With the above aim, comparative expression profiles of the HSF family in a HS tolerant (CLN1621L) and a sensitive cultivar (CA4) of tomato under HS revealed cultivar-biased regulation of an activator (HSFA7a) and repressor (HSFB4a) class HSF. Functional characterization of HSFA7a that was strongly up-regulated in the tolerant cultivar by VIGS-based silencing and overexpression established it as a positive regulator of HS-tolerance. While knock-down and overexpression analyses of HSFB4a that was down-regulated in CLN1621L in HS, showed it as a negative regulator of thermotolerance. Promoter:GUS reporter assays and promoter sequence analyses suggest heat-mediated transcriptional control of both the HSF genes in the contrasting cultivars. Moreover, we show HSFB4a is also regulated post-transcriptionally by microRNA Sly-miR4200 using degradome, short-tandem-target-mimic of Sly-miR4200 and transient in-planta Sly-miR4200-effector:HSFB4a-reporter assays. This miRNA is induced several folds upon HS in the tolerant variety thereby reducing HSFB4a levels. We thus propose that the alleviation of HSFB4a repressor governs thermotolerance in the tolerant cultivar by regulating downstream heat stress responsive genes.

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Section: Methodsmentioning

confidence: 99%

Transcriptional regulation ofHSFA7and post-transcriptional modulation ofHSFB4aby miRNA4200 govern general and varietal thermotolerance in tomato

Rao

Balyan

Verma

et al. 2021

Preprint

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“…2). In contrast, since drought gradually increases HY5 expression [31], SlHY5 overexpressing lines decreased H2O2 and MDA accumulation compared to WT, as their enzymatic antioxidant defense systems (eg SUPEROXIDE DISMUTASE, SOD; and CAT) were enhanced [32]. Thus the functional SlCRY1a in the WT likely positively regulates the expression of SlHY5, thereby promoting the activity of antioxidant enzymes thus decreasing membrane lipid peroxidation during soil drying.…”

Section: Blue Light Activates Crys To Positively Regulate Downstream Expression Of the Hy5mentioning

confidence: 97%

“…root growth [42] and stomatal closure [43]. HY5 is a candidate molecule to regulate H2O2 root-shoot communication by interacting with CRY1a activity in tomato [30,32], since local activity (shoot or root) of HY5 transcription factor (protein) mediates downstream mobile signals from shoots to roots in Arabidopsis regulating plant growth [44], and is likely important in CRY1a mediated root-shoot communication in tomato (Fig. 10).…”

Section: Importance Of Tomato Cry1a In Regulating Long-distance-signaling Of Water Deficitmentioning

confidence: 99%

Cryptochrome 1a of tomato mediates long-distance signaling of soil water deficit

et al. 2021

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“…RNA isolation, cDNA synthesis and qRT-PCR were performed as per Paul et al (2016). The expression assays were performed with at least two biological and three technical replicates and normalized to tomato actin gene as endogenous control (Han et al, 2020;Qiu et al, 2016;Yan et al, 2020) using comparative ΔΔCT method.…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

Characterization of novel regulators for heat stress tolerance in tomato from Indian sub‐continent

Balyan

Rao

Jha

et al. 2020

Plant Biotechnology Journal

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Summary The footprint of tomato cultivation, a cool region crop that exhibits heat stress (HS) sensitivity, is increasing in the tropics/sub‐tropics. Knowledge of novel regulatory hot spots from varieties growing in the Indian sub‐continent climatic zones could be vital for developing HS‐resilient crops. Comparative transcriptome‐wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar pair shortlisted from a pool of varieties exhibiting variable thermo‐sensitivity using physiological‐, survival‐ and yield‐related traits revealed redundant to cultivar‐specific HS regulation. The antagonistically expressing genes encode enzymes and proteins that have roles in plant defence and abiotic stresses. Functional characterization of three antagonistic genes by overexpression and silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis) that are up‐regulated in tolerant cultivar, as positive regulators of HS tolerance and Solyc03g020030 (Pin‐II proteinase inhibitor), that are down‐regulated in CLN1621L, as negative regulator of thermotolerance. Transcriptional assessment of promoters of these genes by SNPs in stress‐responsive cis‐elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar‐specific orchestration of transcription factors in regulating transcription. Moreover, overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS tolerance in tomato. This study identifies several novel HS tolerance genes and provides proof of their utility in tomato thermotolerance.

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SlHY5 is a necessary regulator of the cold acclimation response in tomato

Cited by 31 publications

References 58 publications

Transcriptional regulation ofHSFA7and post-transcriptional modulation ofHSFB4aby miRNA4200 govern general and varietal thermotolerance in tomato

Transcriptional regulation ofHSFA7and post-transcriptional modulation ofHSFB4aby miRNA4200 govern general and varietal thermotolerance in tomato

Cryptochrome 1a of tomato mediates long-distance signaling of soil water deficit

Characterization of novel regulators for heat stress tolerance in tomato from Indian sub‐continent

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