SlIAA9 Mutation Maintains Photosynthetic Capabilities under Heat-Stress Conditions

Rahmat, Bayu Pradana Nur; Octavianis, Grace; Budiarto, Rahmat; Jadid, Nurul; Widiastuti, Ani; Matra, Deden Derajat; Ezura, Hiroshi; Mubarok, Syariful

doi:10.3390/plants12020378

Cited by 11 publications

(7 citation statements)

References 55 publications

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“…These mutants and WT-MT as control were cultivated in the greenhouse of the experimental field of the Laboratory of Horticulture, Faculty of Agriculture, Universitas Padjadjaran, with four biological replications; each replication consisted of six individual plants for vegetative analysis. The iaa9-3 and iaa9-5 mutants and WT-MT seeds were sown in plug trays, in accordance with the methods described by Rahmat et al (2023). After the seedling produced 4-5 leaves, they were transplanted into a 12 cmdiameter pot that was filled with a mixture of soil and commercial coir coco peat (1:1/v:v) and cultivated in two greenhouse conditions, i.e.…”

Section: Plant Materials and Cultivation Conditionmentioning

confidence: 99%

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Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions

Mubarok

Jadid²,

Widiastuti³

et al. 2023

Front. Plant Sci.

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Fruit set is one of the main problems that arise in tomato plants under heat-stress conditions, which disrupt pollen development, resulting in decreased pollen fertility. Parthenocarpic tomatoes can be used to increase plant productivity during failure of the fertilisation process under heat-stress conditions. The aim of this study were to identify the plant adaptability and fruiting capability of ?iaa9-3 and iaa9-5 tomato mutants under heat-stress conditions. The iaa9-3 and iaa9-5 and wild-type Micro-Tom (WT-MT) plants were cultivated under two temperature conditions: normal and heat-stress conditions during plant growth. The results showed that under the heat-stress condition, iaa9-3 and iaa9-5 showed delayed flowering time, increased number of flowers, and increased fruit set and produced normal-sized fruit. However, WT-MT cannot produce fruits under heat stress. The mutants can grow under heat-stress conditions, as indicated by the lower electrolyte leakage and H2O2 concentration and higher antioxidant activities compared with WT-MT under heat-stress conditions. These results suggest that iaa9-3 and iaa9-5 can be valuable genetic resources for the development of tomatoes in high-temperature environmental conditions.

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Section: Plant Materials and Cultivation Conditionmentioning

confidence: 99%

“…Environmental temperatures above 35 °C in tomato cultivation damage plant growth and development, including seed germination, vegetative and reproductive growth, fruit setting and photosynthetic activity (Ariizumi et al, 2013;Rahmat et al, 2023). The severity of heat stress depends on the duration and time of heat-stress exposure.…”

Section: Introductionmentioning

confidence: 99%

Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions

Mubarok

Jadid²,

Widiastuti³

et al. 2023

Front. Plant Sci.

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“…Besides the parthenocarpic phenotype, sliaa9 mutants showed simple leave architecture, which is a convenient, visible marker for breeding and selection on a large scale. However, the performance of the sliaa9 mutants differs depending on the genetic backgrounds and the cultivating conditions(Abe-Hara et al 2021; Rahmat et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system

Nguyen,

Van Vu,

Shelake

et al. 2024

Mol Breeding

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Tomato (Solanum lycopersicum L.) is one of the most important crops in the world for its fruit production. Advances in cutting-edge techniques have enabled the development of numerous critical traits related to the quality and quantity of tomatoes. Genetic engineering techniques, such as gene transformation and gene editing, have emerged as powerful tools for generating new plant varieties with superior traits. In this study, we induced parthenocarpic traits in a population of elite tomato (ET) lines. At rst, the adaptability of ET lines to genetic transformation was evaluated to identify the best-performing lines by transforming the SlANT1 gene overexpression cassette and then later used to produce the SlIAA9 knockout lines using the CRISPR/Cas9 system. ET5 and ET8 emerged as excellent materials for these techniques and showed higher e ciency. Typical phenotypes of knockout sliaa9 were clearly visible in G0 and G1 plants, in which simple leaves and parthenocarpic fruits were observed. The high e ciency of the CRISPR/Cas9 system in developing new tomato varieties with desired traits in a short period was demonstrated by generating T-DNA-free homozygous sliaa9 knockout plants in the G1 generation. Additionally, a simple arti cial fertilization method was successfully applied to recover seed production from parthenocarpic plants, securing the use of these varieties as breeding materials.

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“…Besides the parthenocarpy phenotype, iaa9 mutants showed simple leave architecture, which is a convenient visible marker for breeding and selection on a large scale. However, the performance of the iia9 mutants differs depending on the genetic backgrounds and the culturing conditions (Abe-Hara et al 2021;Rahmat et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system

Nguyen

Kim

et al. 2023

Preprint

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Tomato (Solanum lycopersicum L.) is one of the most important crops in the world for its fruit production. Many critical characteristics related to the quality and quantity of tomatoes have been developed using cutting-edge techniques. Recently, genetic techniques such as gene transformation and gene editing have emerged as powerful tools to promptly generate new plant varieties with superior traits. In this study, we successfully induced parthenocarpy traits in a population of elite tomato (ET) lines. The ET lines were screened for their adaptability to new genetic techniques to determine the lines that performed best by applying ANT1 gene transformation and IAA9 knock-out using the CRISPR/Cas9 system. ET5 and ET8 emerged as excellent materials for these techniques due to their high efficiency in applying those modern techniques. Phenotypes of knock-out iaa9 were clearly shown in T-0 and T-1 plants, in which simple leaves and parthenocarpic fruits were observed. We generated T-DNA-free homozygous iaa9 knock-out plants in the T-1 generation, indicating the high efficiency of CRISPR/Cas9 in developing new varieties with desired traits in the shortest time possible. Additionally, a simple artificial fertilization method was successfully applied to recover seed production from parthenocarpy plants, securing the varieties for use as breeding materials.

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SlIAA9 Mutation Maintains Photosynthetic Capabilities under Heat-Stress Conditions

Cited by 11 publications

References 55 publications

Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions

Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions

Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system

Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system

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