Slow non‐cholinergic excitatory potentials in neurones of the guinea‐pig coeliac ganglia.

Dun, Nae J.; C, R

doi:10.1113/jphysiol.1984.sp015231

Cited by 25 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to conventional cholinergic ESPs, slow synaptic responses have been reported to occur in neurones of the coeliac ganglion (Dun & Ma, 1984) following repetitive activation of incoming axons. Trains of stimuli at 15-30 Hz, 200 ms torecruit all fast ESPs (see also Dun & Ma, 1984) and had a very variable time course, commonly peaking at about 1 s after the train and decaying over periods ranging up to minutes.…”

Section: Slow Responses To Repetitive Nerve Stimulimentioning

confidence: 99%

Characteristics of synaptic input to three classes of sympathetic neurone in the coeliac ganglion of the guinea‐pig.

McLachlan

Meckler

1989

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Intracellular recordings from sympathetic neurones in the isolated coeliac ganglion of guinea-pigs have been used to define the synaptic input to three subtypes of neurone, classified on the basis of their discharge during maintained depolarizing current as phasic neurones, neurones with prolonged after-hyperpolarizations (LAH), and tonic neurones.2. The three classes of neurone were distributed characteristically in different parts of the ganglion.3. Passive membrane properties differed between the three neurone types. Mean input resistance was highest in phasic neurones and was inversely related to the size of the prolonged calcium-activated potassium conductance in LAH neurones. Mean input time constant was highest in tonic neurones, because of significantly higher cell capacitance.4. Phasic and LAH neurones usually received one suprathreshold ('strong') as well as several subthreshold excitatory synaptic potentials (ESPs) from the ipsilateral splanchnic nerve. In general, the amplitude and number of splanchnic inputs were greater, and the occurrence of two strong inputs more common, in phasic than in LAH neurones. The input to tonic neurones was small and usually subthreshold, even with supramaximal splanchnic stimulation. In a few (mostly tonic) neurones lying close to the midline, small ESPs were evoked by contralateral splanchnic stimulation.5. Antidromic action potentials were evoked in more than half of all neurones by high voltage coeliac nerve stimulation. In addition, multiple small subthreshold ESPs were recorded in virtually all tonic neurones (99 %) on coeliac nerve stimulation. In contrast, coeliac stimulation rarely evoked a few very small ESPs in LAH neurones (9%), but no synaptic response in phasic neurones.6. In about half of the tonic neurones tested (but no phasic or LAH neurones), small ESPs were evoked by stimulation of the intermesenteric nerve.7. Slow depolarization elicited by repetitive activation of splanchnic and coeliac nerve trunks, at voltages supramaximal for the fast cholinergic responses, were * Present address: Department of Physiology and Pharmacology, University of Queensland, St Lucia, Queensland 4067, Australia. E. M. McLACHLAN AND R. L. MECKLERrecorded from about half of both phasic and tonic neurones, but only one of twentyfour LAH neurones. These responses commonly faded during subsequent trials, so that it was difficult to characterize them.8. The data indicate that the three broad groups of coeliac neurone, classified on the basis of their voltage-and calcium-dependent potassium conductances, receive different patterns of synaptic input. The differences may be related to the three major functions of vasoconstriction, motility and mucosal secretion in the small intestine.

show abstract

Section: Slow Responses To Repetitive Nerve Stimulimentioning

confidence: 99%

Characteristics of synaptic input to three classes of sympathetic neurone in the coeliac ganglion of the guinea‐pig.

McLachlan

Meckler

1989

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…described in the preceding paper (Dun & Ma, 1984). Three lines of evidence developed from our experiments suggest that serotonin is the mediator of the non-cholinergic e.p.s.p.…”

Section: Discussionmentioning

confidence: 82%

“…was not contaminated by a muscarinic e.p.s.p. (Dun & Ma, 1984). The following agents were used: atropine sulphate, cyproheptadine hydrochloride, fluoxetine hydrochloride (Eli Lilly and Co.), serotonin creatinine sulphate, substance P, L-tryptophan, d-tubocurarine chloride and methysergide maleate (Sandoz Inc.).…”

Section: Methodsmentioning

confidence: 99%

“…Electrophysiological techniques for single-cell recording from neurones of the isolated coeliac superior mesenteric plexus of the guinea-pig were described in the preceding paper (Dun & Ma, 1984). Appropriate pharmacological agents were dissolved in Krebs solution and applied in known concentrations to the ganglia by superfusion.…”

Section: Methodsmentioning

confidence: 99%

“…It was shown in the preceding paper that membrane hyperpolarization increased the amplitude of the non-cholinergic e.p.s.p. in the large majority of cells investigated (Dun & Ma, 1984 Fig. 3.…”

Section: Serotonin Depolarizationmentioning

confidence: 99%

See 2 more Smart Citations

Evidence for a serotonin‐mediated slow excitatory potential in the guinea‐pig coeliac ganglia.

Dun¹,

Kiraly²,

Ma³

1984

The Journal of Physiology

Self Cite

View full text Add to dashboard Cite

SUMMARY1. The nature of the putative transmitters) mediating the non-cholinergic excitatory post-synaptic potential (e.p.s.p.) described in the preceding paper was investigated by means of electrophysiological, pharmacological and immunohistochemical methods.2. Serotonin (1-10 ,SM) when applied by superfusion caused a slow depolarization that closely mimicked the synaptic response in about 60 % of the coeliac neurones that exhibited a non-cholinergic e.p.s.p. The serotonin depolarization evoked in low-Ca2+, highMg2+ solution or in a Krebs solution containing cholinergic antagonists was quantitatively similar to that elicited in normal Krebs solution.3. When compared in the same neurones the membrane resistance change during the course of the serotonin depolarization and of the non-cholinergic e.p.s.p., as well as their respective responses to conditioning polarization, were similar.4. The non-cholinergic e.p.s.p. was reversibly abolished during serotonin-induced depolarization; the blockade persisted when the membrane potential was restored to the resting level by hyperpolarizing current.5.

show abstract

Physiology of prevertebral ganglia in mammals with special reference to inferior mesenteric ganglion

Szurszewski¹,

King²

1989

Comprehensive Physiology

View full text Add to dashboard Cite

Slow non‐cholinergic excitatory potentials in neurones of the guinea‐pig coeliac ganglia.

Cited by 25 publications

References 20 publications

Characteristics of synaptic input to three classes of sympathetic neurone in the coeliac ganglion of the guinea‐pig.

Characteristics of synaptic input to three classes of sympathetic neurone in the coeliac ganglion of the guinea‐pig.

Evidence for a serotonin‐mediated slow excitatory potential in the guinea‐pig coeliac ganglia.

Physiology of prevertebral ganglia in mammals with special reference to inferior mesenteric ganglion

Contact Info

Product

Resources

About