Slowly Progressive Osteoarthritis After Tibial Valgus Osteotomy in Young Beagle Dogs

Panula, Harri E; Helminen, Heikki J.; Kiviranta, Ilkka

doi:10.1097/00003086-199710000-00030

Cited by 27 publications

(28 citation statements)

References 13 publications

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“…In each group the operated and a control dogs were littermates. A slowly progressive OA was induced in the right knee (stifle) joint of the dogs by a method described earlier 25. Briefly, a 30° valgus angulation to the right tibia was created by operation in 15 dogs at the age of 3 months.…”

Section: Methodsmentioning

confidence: 99%

“…The 5 μm thick sections were stained after dewaxing and rehydration with Weigert’s iron haematoxylin, safranin O, and fast green 25. Mankin’s scores were assessed from the histological sections as described earlier 25…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we have reported the effects of 30° valgus angulation of tibia (valgus osteotomy) to induce slowly progressive OA cartilage lesions in stable knee (stifle) joints of young female beagle dogs 25. In this study we have used this model and quantitative polarised light microscopy to investigate the collagen network organisation, thickness of the birefringent zones, and PG concentration in the macroscopically intact articular cartilage.…”

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confidence: 99%

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Articular cartilage superficial zone collagen birefringence reduced and cartilage thickness increased before surface fibrillation in experimental osteoarthritis

Panula

Hyttinen

Arokoski

et al. 1998

Annals of the Rheumatic Diseases

128

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Objectives-To investigate articular cartilage collagen network, thickness of birefringent cartilage zones, and glycosaminoglycan concentration in macroscopically normal looking knee joint cartilage of young beagles subjected to experimental slowly progressive osteoarthritis (OA). Methods-OA was induced by a tibial 30°v algus osteotomy in 15 female beagles at the age of 3 months. Fifteen sisters were controls. Cartilage specimens were collected seven (Group 1) and 18 months (Group 2) postoperatively. Collagen induced optical path diVerence and cartilage zone thickness measurements were determined from histological sections of articular cartilage with smooth and intact surface by computer assisted quantitative polarised light microscopy. Volume density of cartilage collagen fibrils was determined by image analysis from transmission electron micrographs and content of glycosaminoglycans by quantitative digital densitometry from histological sections. Results-In the superficial zone of the lateral tibial and femoral cartilage, the collagen induced optical path diVerence (birefringence) decreased by 19 to 71% (p < 0.05) seven months postoperatively. This suggests that severe superficial collagen fibril network deterioration took place, as 18 months postoperatively, macroscopic and microscopic OA was present in many cartilage areas. Thickness of the uncalcified cartilage increased while the superficial zone became thinner in the same sites. In operated dogs, glycosaminoglycan content first increased (Group 1) in the lateral tibial condyle and then decreased (Group 2) (p < 0.05). Conclusion-In this OA model, derangement of the superficial zone collagen network was the probable reason for birefringence reduction. This change occurred well before macroscopic OA.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Articular cartilage superficial zone collagen birefringence reduced and cartilage thickness increased before surface fibrillation in experimental osteoarthritis

Panula

Hyttinen

Arokoski

et al. 1998

Annals of the Rheumatic Diseases

128

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“…Common methods are meniscectomy and transection of the cranial cruciate ligament (CCL), which result in a true instability of the joint leading to OA (Roos et al, 1995). Alterations of the joint load by tibial osteotomy (Panula et al, 1997), by immobilization (Leroux et al, 2001) are other used physical stifle OA models. In the CCL transected model, permanent instability in the stifle joint is followed by degenerative changes in cartilage and synovial tissues in dogs (Brandt et al, 1991).…”

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confidence: 99%

Surgical model of osteoarthritis secondary to medial patellar luxation in dogs

Alam¹,

Lee²,

Kim³

et al. 2011

Vet. Med.

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ABSTRACT:This study was performed to make a surgical model of osteoarthritis (OA) in the dog. Experimental medial patellar luxation (MPL) was surgically produced in the left stifle (index) of 24 skeletally mature mixed small breed dogs (age two to six years and weight 2.8 to 9 kg). The animals were randomly allocated in 2 groups; sham group (n = 12), where the right stifle was sham operated and control group (n = 12) with intact right stifle. Physical and radiographic examinations of both stifles were performed at 1.5 months intervals over a one-year experimental period. One dog was euthanatized every three months, and both stifles were explored, gross examination was performed and tissue samples from the articular cartilage, cranial cruciate ligament (CCL) and synovium were collected for histomorphology. The clinical signs of OA were obvious in the experimental dogs by 12 weeks of surgical induction of MPL, which was also evidenced in the histopathology of the joint tissues and electron microscopy of the articular cartilage. The radiographic changes of OA were not obvious until remarkable degenerative changes became abvious six months postoperatively. Surgically induced MPL can be a successful tool for experimental induction of OA in dogs.

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“…The system has been widely used in animal studies on osteoarthrosis (Panula et al 1994(Panula et al , 1998. The capability of the system has been demonstrated, and it has been found useful for systematic assessment of articular cartilage, as the categories included in the scoring system encompass highly relevant histological and histochemical variables.…”

Section: Discussionmentioning

confidence: 99%

Articular cartilage degeneration after acute subchondral bone damage An experimental study in dogs with histopathological grading

Lahm

Uhl

Erggelet

et al. 2004

Acta Orthopaedica Scandinavica

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Background Subchondral fracture patterns and bone bruises have been described and some clinical studies have shown alterations in the initially healthy cartilage after such lesions.Methods and results After having performed cadaver studies, we created an animal model to produce pure subchondral damage without affecting the articular cartilage, under MRI control. We used 12 beagle dogs. For quantification of different degrees of staining, we used a grading of the sections by means of the HHGS (Histological-Histochemical Grading System) or Mankin score.Results In all cases, FLASH 3D sequences revealed intact cartilage in MRI after impact. The best detection of subchondral fractures was achieved in fat-suppressed TIRM sequences. Image analysis based on the HHGS showed changes in 10 of 12 samples, with a high degree of significance 6 months after the initial trauma. Correlation analysis showed loss of the physiological distribution of proteoglycans and glycoproteins in the different zones of articular cartilage. Subcategories "Structure", "Cells" and "Safranin-O Staining" also showed high significance, and the category "Tidemark Integrity" showed a tendency. Interpretation Our findings indicate that acute subchondral fractures are a predictor of degenerative changes within 6 months. Modifications and supplements to rehabilitation might be needed in cases with accompanying subchondral lesions, e.g. in ACL tears.

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Slowly Progressive Osteoarthritis After Tibial Valgus Osteotomy in Young Beagle Dogs

Cited by 27 publications

References 13 publications

Articular cartilage superficial zone collagen birefringence reduced and cartilage thickness increased before surface fibrillation in experimental osteoarthritis

Articular cartilage superficial zone collagen birefringence reduced and cartilage thickness increased before surface fibrillation in experimental osteoarthritis

Surgical model of osteoarthritis secondary to medial patellar luxation in dogs

Articular cartilage degeneration after acute subchondral bone damage An experimental study in dogs with histopathological grading

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