Slt2 (Mpk1) MAP kinase is involved in the response of Saccharomyces cerevisiae to 8-methoxypsoralen plus UVA

Dardalhon, M.; Agoutin, Bernadette; Watzinger, Malene; Averbeck, D.

doi:10.1016/j.jphotobiol.2009.02.001

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…The simultaneous activation of all of these pathways may lead to several options for the cell, including repair and cell death, depending on many critical factors and the interaction of different pathways. Our qRT-PCR results showed that PDI treatment greatly enhances antioxidant activity and alters the gene expression levels of other unrelated oxidative stress genes, thereby suggesting that PDI treatment elicits a diverse range of cellular responses, as was demonstrated in previous studies (25)(26)(27)(28)(29)(30).…”

Section: Transcriptional Analysis Using Quantitative Real-time Pcrsupporting

confidence: 78%

“…Photodynamic therapy (PDT) induces a cascade of molecular events in human tumors, including activation of transcription factors, heat shock proteins, antioxidant enzymes, and apoptotic pathways, as well as altered cell attachment and protein transport, which can lead to cell death (25–27). Currently, the underlying molecular events involved in cell death after PDI are poorly defined in pathogenic fungus (28–30). However, it is known that transcriptional activation in P. brasiliensis plays an important role in the molecular response to PDI, and therefore, further study of this fungus will help broaden our knowledge of PDT.…”

Section: Introductionmentioning

confidence: 99%

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Cell Survival and Altered Gene Expression Following Photodynamic Inactivation of Paracoccidioides brasiliensis

Almeida

Zanoelo

Castro

et al. 2012

Photochem & Photobiology

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Paracoccidioidomycosis (PCM) is a systemic mycosis caused by Paracoccidioides brasiliensis. Currently, the treatment approach involves the use of antifungal drugs and requires years of medical therapy, which can induce nephrotoxicity and lead to resistance in yeast strains. Photodynamic inactivation (PDI) is a new therapy capable of killing microorganisms via the combination of a nontoxic dye with visible light to generate toxic reactive oxygen species (ROS). We investigated the phototoxic effect of 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin (TMPyP), a cationic porphyrin, on the survival of P. brasiliensis following exposure to light. Phototoxicity was found to depend on both the fluence and concentration of the photosensitizer (PS). Although the biological effects of PDI are known, the molecular mechanisms underlying the resultant damage to cells are poorly defined. Therefore, we evaluated the molecular response to PDI-induced oxidative stress by gene transcription analysis. We selected genes associated with the high-osmolarity glycerol (HOG)-mitogen-activated protein kinase (MAPK) pathway and antioxidant enzymes. The genes analyzed were all overexpressed after PDI treatment, suggesting that the oxidative stress generated in our experimental conditions induces antioxidant activity. In addition to PDI-induced gene expression, there was high cell mortality, suggesting that the antioxidant response was not sufficient to avoid fungal mortality.

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Section: Transcriptional Analysis Using Quantitative Real-time Pcrsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Cell Survival and Altered Gene Expression Following Photodynamic Inactivation of Paracoccidioides brasiliensis

Almeida

Zanoelo

Castro

et al. 2012

Photochem & Photobiology

View full text Add to dashboard Cite

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“…Current understanding of each of the inputs and outputs of this pathway related to the maintenance of cell wall integrity will be discussed individually. Although the CWI pathway has additionally been implicated in the responses to oxidative stress (Alic et al 2003;Vilella et al 2005), high and low pH stress (Claret et al 2005;Serrano et al 2006), and DNA damage (Queralt and Igual 2005;Dardalhon et al 2009;Truman et al 2009;Bandyopadhyay et al 2010), this review article will be restricted to its role in the maintenance of cell wall integrity.…”

Section: Overview Of Cwi Signalingmentioning

confidence: 99%

Regulation of Cell Wall Biogenesis in Saccharomyces cerevisiae: The Cell Wall Integrity Signaling Pathway

2011

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The yeast cell wall is a strong, but elastic, structure that is essential not only for the maintenance of cell shape and integrity, but also for progression through the cell cycle. During growth and morphogenesis, and in response to environmental challenges, the cell wall is remodeled in a highly regulated and polarized manner, a process that is principally under the control of the cell wall integrity (CWI) signaling pathway. This pathway transmits wall stress signals from the cell surface to the Rho1 GTPase, which mobilizes a physiologic response through a variety of effectors. Activation of CWI signaling regulates the production of various carbohydrate polymers of the cell wall, as well as their polarized delivery to the site of cell wall remodeling. This review article centers on CWI signaling in Saccharomyces cerevisiae through the cell cycle and in response to cell wall stress. The interface of this signaling pathway with other pathways that contribute to the maintenance of cell wall integrity is also discussed.

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“…Some metabolites are genetically induced by some essential oils. Lower concentrations of the cytotoxic effect can induce the production of secondary metabolites in Saccharomyces cerevisiae (29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

Stimulato-Inhibitory Response to Cumin Oil in Aflatoxin B1 Production of Aspergillus Species

Fakoor

Rasooli

Owlia

et al. 2012

Jundishapur J Microbiol

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Background: Aspergillus species produce the highly toxic and carcinogenic metabolite, Aflatoxin B1 (AFB1), on food and agricultural commodities. Some natural products are known to inhibit aflatoxin production. Objectives: With the aim of controlling aflatoxin production, the essential oils of Cuminum cyminum L. from the best known regions of Iran i.e. Alborz Mountain and Kerman region, were obtained by hydrodistillation. Materials and Methods: Antifungal activities of the oils to inhibit growth and aflatoxin productivity of A. flavus PICC-AF39, A. flavus PICC-AF24, and A. parasiticus NRRL-2999 were studied. Minimal inhibitory (MIC) and minimal fungicidal (MFC) concentrations of the oil were determined. Sub-MIC was selected for the measurement of aflatoxins B and G concentration. Samples were analyzed either using a high performance liquid chromatography (HPLC) method with some minor modifications. Aflatoxins (AFs) were determined by reverse-phase HPLC and fluorescence detector with post column derivatization (PCD) involving bromination.

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Slt2 (Mpk1) MAP kinase is involved in the response of Saccharomyces cerevisiae to 8-methoxypsoralen plus UVA

Cited by 9 publications

References 47 publications

Cell Survival and Altered Gene Expression Following Photodynamic Inactivation of Paracoccidioides brasiliensis

Cell Survival and Altered Gene Expression Following Photodynamic Inactivation of Paracoccidioides brasiliensis

Regulation of Cell Wall Biogenesis in Saccharomyces cerevisiae: The Cell Wall Integrity Signaling Pathway

Stimulato-Inhibitory Response to Cumin Oil in Aflatoxin B1 Production of Aspergillus Species

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