SMAD2 regulates testicular development and testosterone synthesis in Hu sheep

Yang, Hua; Wan, Zhen; Jin, Yecheng; Feng, Weiyue

doi:10.1016/j.theriogenology.2021.08.027

Cited by 9 publications

(4 citation statements)

References 58 publications

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“…In order to assess the involvement of DE-circRNAs in the regulation of sperm motility, we chose one circRNA downregulated in HFD SPZ and carried out the construction of a ceRNET in order to shed light on predicted mRNAs potentially regulating sperm motility pathways. We chose circSMAD2 since its linear transcript encodes for a protein involved in testosterone production as well as germ cell proliferation and apoptosis inhibition [ 38 ]. In addition, SMAD2 was reported to be mainly present in the cytoplasm of germ cells, and changes in its expression levels were associated with decreased sperm production in rats [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Novel Insights into circRNA Saga Coming from Spermatozoa and Epididymis of HFD Mice

Manfrevola

Chioccarelli

Mele

et al. 2023

IJMS

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Obesity is a pathophysiological disorder associated with adiposity accumulation, oxidative stress, and chronic inflammation state that is progressively increasing in younger population worldwide, negatively affecting male reproductive skills. An emerging topic in the field of male reproduction is circRNAs, covalently closed RNA molecules produced by backsplicing, actively involved in a successful spermatogenesis and in establishing high-quality sperm parameters. However, a direct correlation between obesity and impaired circRNA cargo in spermatozoa (SPZ) remains unclear. In the current work, using C57BL6/J male mice fed with a high-fat diet (HFD, 60% fat) as experimental model of oxidative stress, we investigated the impact of HFD on sperm morphology and motility as well as on spermatic circRNAs. We performed a complete dataset of spermatic circRNA content by a microarray strategy, and differentially expressed (DE)-circRNAs were identified. Using a circRNA/miRNA/target network (ceRNET) analysis, we identified circRNAs potentially involved in oxidative stress and sperm motility pathways. Interestingly, we demonstrated an enhanced skill of HFD sperm in backsplicing activity together with an inefficient epididymal circRNA biogenesis. Fused protein in sarcoma (FUS) and its ability to recruit quaking (QKI) could be involved in orchestrating such mechanism.

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Section: Resultsmentioning

confidence: 99%

Novel Insights into circRNA Saga Coming from Spermatozoa and Epididymis of HFD Mice

Manfrevola

Chioccarelli

Mele

et al. 2023

IJMS

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“…Previous mouse model studies have shown that deletion of SMAD4 and FOXL2 in GnRH results in FSH deficiency and female infertility [47], and FSH and LH can enhance the activity of the TGF-β/SMAD signaling pathway [48]. Moreover, SMAD2 downregulation could promote cell apoptosis and inhibit cell proliferation, thereby suppressing testosterone levels in sheep Leydig cells [49]. In our study, we determined that SMAD2 is a potential downstream regulator of oar-miR-16b by dual-luciferase reporter analysis.…”

Section: Discussionmentioning

confidence: 99%

The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway

Wan

Yang

Chen

et al. 2022

Cells

Self Cite

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Pituitary gonadotropins play a pivotal role in reproduction. Long noncoding RNAs (lncRNAs) have been identified as important regulators in the hypothalamic–pituitary–ovarian (HPO) axis associated with reproduction. However, the contributions of lncRNAs to pituitary gonadotropin secretion remain largely unknown. Therefore, this work was performed to uncover the functional mechanisms of the novel lncRNA TCONS_00083279 (lncRNA SM2) and its potential targeting pathway oar-miR-16b/TGF-beta/SMAD2, which is associated with gonadotropin secretion in sheep pituitary cells. In the present study, the lncRNA SM2 showed high expression levels in the sheep pituitary gland, and it was located in both the nucleus and the cytoplasm of pituitary cells. lncRNA SM2 knockdown inhibited pituitary cell proliferation and FSH and LH secretion. The function of the lncRNA SM2 was sponged by oar-miR-16b, and this regulated the growth and gonadotropin secretion of pituitary cells by modulating SMAD2, as shown by the dual-luciferase reporter assay. FSH and LH levels were both upregulated by SMAD2 overexpression. Moreover, the levels of the lncRNA SM2, SMAD2 and TGFR1, as well as FSH and LH, in sheep pituitary cells increased significantly under gonadotropin-releasing hormone (GnRH) stimulation (p < 0.05). This work illustrates that the lncRNA SM2 regulates gonadotropin secretion in the Hu sheep anterior pituitary by targeting the oar-miR-16b/TGF-β/SMAD2 signaling pathway, providing a valuable resource for understanding the molecular mechanisms underlying sheep reproduction.

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“…The Hu sheep is a unique breed of sheep in China, known for its early sexual maturity, rapid growth and development, high fecundity, suitability for indoor rearing, strong adaptability to various environments, and excellent meat production performance [ 14 ]. China has a large population and limited land, leading to a growing demand for mutton [ 15 ]. As a result, indoor sheep farming has become the predominant method of breeding, and the Hu sheep breed aligns with this current development trend.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation

Zhang,

Wang,

Jiang

et al. 2024

Animals

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The dilution method and ratio were tested to assess their effects on the Hu ram semen after cryopreservation. Experiment I aimed to explore the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I (Tris-based and egg yolk) under the condition of 1:1 dilution of diluent II (diluent I and glycerol) on the Hu ram semen preserved in liquid nitrogen regarding spermatozoa motility and kinetic parameters. Experiment II aimed to investigate the effect of various dilution ratios (1:1, 1:2, 1:3, 1:4) of diluent I under the condition of 1:2 dilution of diluent II to the Hu ram semen for cryopreservation on spermatozoa motility and kinetic parameters. The purpose of experiment III is to assess the effect of various dilution methods and ratios on the cryopreservation of Hu ram semen by detecting spermatozoa motility, kinetic parameters, plasma membrane integrity, acrosome integrity and reactive oxygen species (ROS) level. Experiment III includes four groups: one-step dilution method and two-step dilution method. The two-step dilution method includes two groups: 1:2, 1:1 and 1:3, 1:2, and the one-step dilution method includes two groups: 1:5 and 1:11. The results indicated that the post-thawed spermatozoa total motility (TM), progressive motility (PM) and average motion degree (MAD) were highest in the 1:2 group and significantly higher (p < 0.05) than those in the 1:1 and 1:4 groups under the condition of 1:1 dilution of diluent II. The post-thawed spermatozoa TM and PM of the 1:3 group were significantly higher (p < 0.05) than those of the other groups under the condition of 1:2 dilution of diluent II. The post-thawed spermatozoa TM, PM, plasma membrane integrity and acrosome integrity of the two-step group (1:3, 1:2) were the highest and significantly higher (p < 0.05) than those in the other groups. Additionally, the post-thawed spermatozoa ROS level of the two-step group (1:3, 1:2) was significantly lower (p < 0.05) than that in the one-step groups (1:5 and 1:11). Therefore, a two-step dilution (1:3, 1:2) was found to be the most suitable method and ratio for diluting the Hu ram semen after cryopreservation.

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SMAD2 regulates testicular development and testosterone synthesis in Hu sheep

Cited by 9 publications

References 58 publications

Novel Insights into circRNA Saga Coming from Spermatozoa and Epididymis of HFD Mice

Novel Insights into circRNA Saga Coming from Spermatozoa and Epididymis of HFD Mice

The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway

Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation

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