Small animal models to understand pathogenesis of osteoarthritis and use of stem cell in cartilage regeneration

Piombo, Virginia

doi:10.1002/cbf.3246

Cited by 4 publications

(3 citation statements)

References 124 publications

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“…The effect of age: by injecting adipose stem cells into the knee joints of elderly patients suffering from knee arthritis, combined with arthroscopic minimally invasive surgery for joint cavity lavage, the clinical feasibility and safety of human adipose stem cells in the treatment of degenerative cartilage diseases have been studied. The result of the treatment is to improve body function and promote articular cartilage repair, thereby alleviating the pain of the patient's knee joint [ 19 ]. However, similar to the combined injection of bone marrow mesenchymal stem cells, this technology also has some similar problems.…”

Section: Osteoarticular Cartilage Regenerationmentioning

confidence: 99%

Stem Cells Promote the Regeneration of Knee Joint Degenerative Bone and Articular Cartilage

Zheng

2022

Journal of Healthcare Engineering

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Cartilage damage has a certain ability to spontaneously repair, but the repaired tissue often shows the phenomenon of cartilage terminal differentiation, which causes irreversible damage to its structure and function and seriously affects the quality of life and work of patients. It is of great significance to study the problems encountered in the process of cartilage damage repair. This article mainly studied stem cells to promote the regeneration of knee joint degenerative bone articular cartilage. First, the animal articular cartilage defect is modeled, 10 ml of animal venous blood is drawn, 0.5 ml of PRP is collected by centrifugation, mixed with cartilage fragments, and transplanted into the defect area into a gel. In the BMSCs group, 1 ml of BMSCs with a cell concentration of 107 cells/ml was injected intra-articularly. The histological chromosomes were observed after 6 weeks and 12 weeks, and the effect of cartilage tissue repair was analyzed and evaluated, and the related data were statistically analyzed. We evaluated the spontaneous repair ability of partial cartilage damage, full-thickness cartilage damage, and osteochondral damage. Furthermore, for partial cartilage damage repair, by using the cartilage damage in vitro model and biomaterials to simulate the in vivo microenvironment, the adhesion and cell morphology on the surface of partial- and full-thickness cartilage damage were evaluated, and the experiments were further used to evaluate the exogenous and internal induced migration effect of source on cultured cells in vitro. In the cell concentration study, the cartilage repair effect increased with the increase in concentration within a certain range, and the tissue repair ability remained stable when the concentration exceeded 107 cells/ml. Using ECM-oriented scaffolds to compound autologous BMSCs, tissue-engineered cartilage was successfully constructed, which had the histological and biochemical characteristics of normal cartilage tissue, and better repaired the damaged articular cartilage of large animals.

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Section: Osteoarticular Cartilage Regenerationmentioning

confidence: 99%

Stem Cells Promote the Regeneration of Knee Joint Degenerative Bone and Articular Cartilage

Zheng

2022

Journal of Healthcare Engineering

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“…Surgical techniques, such as meniscectomy and cruciate ligament resection, mimic the natural progression of OA by creating an unstable condition in the joints [ 175 , 176 ]. Recent studies have shown that large animal models generated more similar clinical OA pathological changes than small animals [ 177 ].…”

Section: Articular Cartilage Regeneration Under Abnormal Conditionsmentioning

confidence: 99%

Instructive cartilage regeneration modalities with advanced therapeutic implantations under abnormal conditions

Wang

et al. 2022

Bioactive Materials

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The development of interdisciplinary biomedical engineering brings significant breakthroughs to the field of cartilage regeneration. However, cartilage defects are considerably more complicated in clinical conditions, especially when injuries occur at specific sites ( e.g. , osteochondral tissue, growth plate, and weight-bearing area) or under inflammatory microenvironments ( e.g. , osteoarthritis and rheumatoid arthritis). Therapeutic implantations, including advanced scaffolds, developed growth factors, and various cells alone or in combination currently used to treat cartilage lesions, address cartilage regeneration under abnormal conditions. This review summarizes the strategies for cartilage regeneration at particular sites and pathological microenvironment regulation and discusses the challenges and opportunities for clinical transformation.

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“…Destabilization of the medial meniscus (DMM) has been widely used to establish animal OA models [ 25 – 29 ]. The meniscus is often regarded to possess features of shock absorption and load distribution.…”

Section: Introductionmentioning

confidence: 99%

Integrin αVβ3 Signaling in the Progression of Osteoarthritis Induced by Excessive Mechanical Stress

Song

Mao²,

Dai

et al. 2022

Inflammation

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Osteoarthritis (OA) is believed to be linked with cartilage degeneration, subchondral bone sclerosis, and synovial inflammation that lead to joint failure, and yet treatment that can effectively reverse the pathological process of the disease still not exists. Recent evidence suggests excessive mechanical stress (eMS) as an essential role in the pathogenesis of OA. Increased levels of integrin αVβ3 have been detected in osteoarthritic cartilage and were previously implicated in OA pathogenesis. However, the role of integrin αVβ3 in the process of eMS-induced OA remains unclear. Here, histologic and proteomic analyses of osteoarthritic cartilage in a rat destabilization of the medial meniscus model demonstrated elevated expression of integrin αVβ3 as well as more serious cartilage degeneration in the medial weight-bearing area. Furthermore, results of in vitro study demonstrated that eMS led to a significant increase of integrin αVβ3 expression and phosphorylation of downstream signaling molecules such as FAK and ERK, as well as upregulated expressions of inflammatory and degradative mediators. In addition, we found that inhibition of integrin αVβ3 could alleviate chondrocyte inflammation triggered by eMS both in vivo and in vitro. Our findings suggest a central role for upregulation of integrin αVβ3 signaling in OA pathogenesis and demonstrate that activation of integrin αVβ3 signaling in cartilage contributes to inflammation and joint destruction in eMS-induced OA. Taken together, our data presented here provide a possibility for targeting integrin αVβ3 signaling pathway as a disease-modifying therapy.

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Small animal models to understand pathogenesis of osteoarthritis and use of stem cell in cartilage regeneration

Cited by 4 publications

References 124 publications

Stem Cells Promote the Regeneration of Knee Joint Degenerative Bone and Articular Cartilage

Stem Cells Promote the Regeneration of Knee Joint Degenerative Bone and Articular Cartilage

Instructive cartilage regeneration modalities with advanced therapeutic implantations under abnormal conditions

Integrin αVβ3 Signaling in the Progression of Osteoarthritis Induced by Excessive Mechanical Stress

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