Small Bait Traps as Accurate Predictors of Dipteran Early Colonizers in Forensic Studies

Farinha, Ana O.; Dourado, Catarina G.; Centeio, Neiva; Oliveira, Ana Rita; Dias, Deodália; Rebelo, María Teresa

doi:10.1673/031.014.77

Cited by 23 publications

(5 citation statements)

References 39 publications

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“…1 ). The most abundant Diptera collected in every session were Muscidae and Calliphoridae specimens, as was also shown by other authors in studies carried out in the Iberian Peninsula with pig [ 36 , 45 , 46 ], dog [ 47 ] and cat (unpublished observations), except in January, when Drosophilidae was the second most collected family. In Portugal, Calliphora vicina and C. vomitoria can be found all year round, especially during the winter and spring seasons, while other species inside the Calliphoridae family are captured from late spring until autumn (e.g., Chrysomya albiceps, Lucilia sericata, L. ampullacea and L. caesar ) [ 47 – 49 ].…”

Section: Resultssupporting

confidence: 81%

“…All the detected species have been previously reported as occurring in Portugal, associated with carrion and organic matter decomposition [e.g. 36 , 46 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

“…From June 2018 onwards, during each rabbit trapping session and within the same site, three modified bottle flytraps [ 36 ] were set baited with rotting meat. Rabbit meat for human consumption was acquired in commercial stores, left to rot for 2–4 days at room temperature, and used as bait.…”

Section: Methodsmentioning

confidence: 99%

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The potential role of scavenging flies as mechanical vectors of Lagovirus europaeus/GI.2

Lopes

Almeida

Diz

et al. 2023

Virol J

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The European rabbit (Oryctolagus cuniculus) populations of the Iberian Peninsula have been severely affected by the emergence of the rabbit haemorrhagic disease virus (RHDV) Lagovirus europaeus/GI.2 (RHDV2/b). Bushflies and blowflies (Muscidae and Calliphoridae families, respectively) are important RHDV vectors in Oceania, but their epidemiological role is unknown in the native range of the European rabbit. In this study, scavenging flies were collected between June 2018 and February 2019 in baited traps at one site in southern Portugal, alongside a longitudinal capture-mark-recapture study of a wild European rabbit population, aiming to provide evidence of mechanical transmission of GI.2 by flies. Fly abundance, particularly from Calliphoridae and Muscidae families, peaked in October 2018 and in February 2019. By employing molecular tools, we were able to detect the presence of GI.2 in flies belonging to the families Calliphoridae, Muscidae, Fanniidae and Drosophilidae. The positive samples were detected during an RHD outbreak and absent in samples collected when no evidence of viral circulation in the local rabbit population was found. We were able to sequence a short viral genomic fragment, confirming its identity as RHDV GI.2. The results suggest that scavenging flies may act as mechanical vectors of GI.2 in the native range of the southwestern Iberian subspecies O. cuniculus algirus. Future studies should better assess their potential in the epidemiology of RHD and as a tool for monitoring viral circulation in the field.

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Section: Resultssupporting

confidence: 81%

“…All the detected species have been previously reported as occurring in Portugal, associated with carrion and organic matter decomposition [e.g. 36 , 46 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The potential role of scavenging flies as mechanical vectors of Lagovirus europaeus/GI.2

Lopes

Almeida

Diz

et al. 2023

Virol J

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“…2007 , Gunn and Bird 2011 ). Such small bait traps have been proven to be accurate predictors of dipteran early colonizers in forensic entomology studies ( Farinha et al. 2014 ).…”

Section: Methodsmentioning

confidence: 99%

An Experimental Investigation Into the Colonization of Concealed Cadavers by Necrophagous Blowflies

2015

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We used seven baited boxes with different combinations of access holes and odor diffusion surfaces to study the arrival of necrophagous flies. During laboratory experiments, 30 gravid Lucilia sericata females were kept in a chamber with one of the boxes. The box with the largest odor diffusion surface (99 cm2) combined with the lowest accessibility (one 1 cm2 entrance hole) was entered least (5 ± 3.7 flies per run). In contrast, the most frequently entered box (one 9 cm2 entrance hole with no additional odor diffusion surface) caught a mean of 24.6 ± 3.4 flies per run. These results indicate that 1) L. sericata entered nearly inaccessible places and 2) both odor diffusion and accessibility impacted the number of flies caught. During field experiments, the seven boxes were placed together outdoors. The box with the most entrances (ten 9-cm2 holes) caught the most flies (55.6–99.4% of the total). Only a few flies entered the other boxes. Access to the less accessible boxes (poor odor diffusion and small entrances) was also delayed. The major conclusions of the field experiments are that 1) boxes with low accessibility took longer to be accessed; 2) larger odor diffusion surfaces were more attractive to flies; and 3) flies accessed boxes more readily through larger holes than through an equivalent surface area made up of smaller holes. With these conclusions in mind, attempts to quantify the preappearance interval or to interpret the number of flies observed in indoor forensic entomology cases should be approached with caution.

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“…We are aware that using small quantities of decomposing material in medicolegal entomology experiments may cause considerable biases, in contrast to using an entire carcass. However, these small portions of carrion are suitable surrogates to reproduce the ecological interactions that occur among the early dipteran colonizers of a carcass or corpse (Farinha et al 2014;Oliveira et al 2015). In addition, we believe that our method, which allows for the occurrence of oviposition in the field, represents an improvement over the laboratory approach to the study of intraguild predation in blow flies.…”

Section: Resultsmentioning

confidence: 99%

Intraguild predation influences oviposition behavior of blow flies (Diptera: Calliphoridae)

et al. 2016

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The objective of the present study was to determine whether blow flies (Diptera: Calliphoridae) are able to identify larvae of an intraguild predator species in the substrate and avoid laying eggs there. Blow flies oviposited in traps with different treatments: substrate only and substrate with larvae of Chrysomya albiceps (Wiedemann, 1819), Chrysomya megacephala (Fabricius, 1794), or Chrysomya putoria (Wiedemann, 1830). Ch. megacephala, Ch. putoria, and Lucilia eximia (Wiedemann, 1819) avoided laying eggs in the trap containing Ch. albiceps larvae. Cochliomyia macellaria (Fabricius, 1775) did not oviposit differently in each substrate but had overall low abundance. The prevalence of species on corpses may be influenced by the ability of the species to detect the presence of other species, mainly predators. In this sense, intraguild predation may result in misinterpretations of a crime scene and should be considered when assessing the minimum postmortem interval.

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Small Bait Traps as Accurate Predictors of Dipteran Early Colonizers in Forensic Studies

Cited by 23 publications

References 39 publications

The potential role of scavenging flies as mechanical vectors of Lagovirus europaeus/GI.2

The potential role of scavenging flies as mechanical vectors of Lagovirus europaeus/GI.2

An Experimental Investigation Into the Colonization of Concealed Cadavers by Necrophagous Blowflies

Intraguild predation influences oviposition behavior of blow flies (Diptera: Calliphoridae)

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